PE/Cyanine7 anti-human Siglec-10 Antibody

Pricing & Availability
Clone
5G6 (See other available formats)
Other Names
Sialic acid binding Ig-like lectin 10
Isotype
Mouse IgG1, κ
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Product Citations
publications
5G6_PE_CYANINE7_Siglec-10_Antibody_070919
Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and Siglec-10 (clone 5G6) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
  • 5G6_PE_CYANINE7_Siglec-10_Antibody_070919
    Human peripheral blood lymphocytes were stained with CD19 Brilliant Violet 421™ and Siglec-10 (clone 5G6) PE/Cyanine7 (left) or mouse IgG1, κ PE/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
347607 25 tests £97
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347608 100 tests £235
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Description

Siglec-10 is a lectin that specifically binds α-2,3- or α-2,6-linked sialic acid. It is a member of the Ig-superfamily and is expressed in monocytes, eosinophils, B cells, and a subset of NK cells. Siglec-10 is a single pass transmembrane protein. The extracellular domain contains one V-set Ig-like domain and three C2-set domains; the cytoplasmic domain contains one immunoreceptor tyrosine-based inhibitor motif (ITIM) which, after tyrosine-phosphorylation, recruits SH2-family phosphatases such as SHP-1 and PTPN6, resulting in the blocking of the signal transduction. CD24 and the vascular adhesion protein-1 (VAP-1) have been described as ligands of Siglec-10.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Siglec-10 fused to human IgG Fc
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Cy7 under optimal conditions. The solution is free of unconjugated PE/Cy7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Munday J, et al. 2001. Biochem. J. 355:489
  2. Yokoi H, et al. 2006. Allergy. 61:769
  3. Kivi E, et al. 2009. Blood 114:5385

Antigen Details

Structure
Lectin, belongs to the Ig-superfamily, has one V-set Ig-like domain and three C2-set domains, a single transmembrane domain and one immunoreceptor tyrosine-based inhibitor motif (ITIM) in the cytoplasmic domain
Distribution

Monocytes, eosinophils, B cells, and a subset of NK cells

Function
Cell adhesion, signal transduction
Interaction
SHP-1, PTPN6
Ligand/Receptor
CD24, vascular adhesion protein-1 (VAP-1)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
B cells, Eosinophils, Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Siglec Molecules
Antigen References

1. Chen GY, et al. 2009. Science 323:1722
2. Aizawa H, et al. 2003. Genomics.82:521
3. Kitzig F, et al. 2002. Biochem Biophys Res Commun. 296:355

Gene ID
89790 View all products for this Gene ID
UniProt
View information about Siglec-10 on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 07/09/2019

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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