APC anti-human CD170 (Siglec-5) Antibody

Pricing & Availability
1A5 (See other available formats)
Regulatory Status
VII 70443
Other Names
Mouse IgG1, κ
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Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 1A5_APC_Siglec-5_Antibody_FC_091014.jpg
    Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
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352005 25 tests £102
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352006 100 tests £240
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CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Reported Reactivity
Chimpanzee, Bonobo, Gorilla
Antibody Type
Host Species
Extracellular region of Siglec-5
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 1A5 antibody cross-reacts with Siglec-144.  Additional reported applications (for the relevant formats) include: ELISA2.

Clone 1A5 cross-reacts to Gorilla2

Application References

(PubMed link indicates BioLegend citation)
  1. Cornish AL, et al. 1998. Blood 92:2123.
  2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
  3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
  4. Angata T, et al. 2006. FASEB J. 20:1964.
  5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
AB_2564261 (BioLegend Cat. No. 352005)
AB_2564262 (BioLegend Cat. No. 352006)

Antigen Details

Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs

Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells

Inhibitory receptor, cell adhesion
SHP-1, SHP-2
α2,3- and α2,6-linked sialic acid, glycophorin A
Cell Type
B cells, Dendritic cells, Granulocytes, Lymphocytes, Macrophages, Monocytes
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.

Gene ID
8778 View all products for this Gene ID
View information about CD170 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/16/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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