APC anti-human CD170 (Siglec-5) Antibody

Pricing & Availability
Clone
1A5 (See other available formats)
Workshop
VII 70443
Other Names
OBBP2, CD33L2, OB-BP2
Isotype
Mouse IgG1, κ
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Product Citations
publications
1A5_APC_Siglec-5_Antibody_FC_091014.jpg
Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
  • 1A5_APC_Siglec-5_Antibody_FC_091014.jpg
    Human peripheral blood granulocytes were stained with Siglec-5 (clone 1A5) APC (filled histogram) or mouse IgG1, κ APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
352005 25 tests £84
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352006 100 tests £198
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Description

CD170, also known as Siglec-5, is a single pass transmembrane protein member of the immunoglobulin superfamily. On the cell surface, CD170 forms a 140 kD dimer. The cytoplasmic domain of Siglec-5 contains two ITIM motifs that recruit the tyrosine-phosphatases SHP-1 and SHP-2 after tyrosine-phosphorylation, which in turn results in the inhibition of cell signaling. Siglec-5 is expressed by granulocytes, monocytes/macrophages, subsets of lymphocytes, and a subset of activated dendritic cells. Siglec-5 binds α2,3- and α2,6-linked sialic acid as well as glycophorin A, and is involved in cell adhesion.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee, Bonobo, Gorilla2
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Extracellular region of Siglec-5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

The 1A5 antibody cross-reacts with Siglec-144.  Additional reported applications (for the relevant formats) include: ELISA2.

Application References

(PubMed link indicates BioLegend citation)
  1. Cornish AL, et al. 1998. Blood 92:2123.
  2. Nguyen DH, et al. 2006. P. Natl. Acad. Sci. USA 103:7765. (FC, ELISA)
  3. Connolly NP, et al. 2002. Br. J. Haematol. 119:221.
  4. Angata T, et al. 2006. FASEB J. 20:1964.
  5. Avril T, et al. 2005. J. Biol. Chem. 280:19843.
RRID
AB_2564261 (BioLegend Cat. No. 352005)
AB_2564262 (BioLegend Cat. No. 352006)

Antigen Details

Structure
Member of the immunoglobulin superfamily, single pass transmembrane protein, forms a 140 kD dimer; cytoplasmic domain contains two ITIM motifs
Distribution
Granulocytes, monocytes/macrophages, subsets of lymphocytes, and activated dendritic cells
Function
Inhibitory receptor, cell adhesion
Interaction
SHP-1, SHP-2
Ligand/Receptor
α2,3- and α2,6-linked sialic acid, glycophorin A
Cell Type
Granulocytes, Monocytes, Macrophages, Lymphocytes, Dendritic cells, B cells
Biology Area
Cell Adhesion, Cell Biology, Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Soto PC, et al. 2010. J. Immunol. 184:4185.
2. Carlin AF, et al. 2009. J. Exp. Med. 206:1691.
3. Zhuravleva MA. 2008. J. Mol. Biol. 375:437.

Gene ID
8778 View all products for this Gene ID
UniProt
View information about CD170 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 09/15/2014

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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