PE/Fire™ 810 anti-human CD335 (NKp46) Antibody

Pricing & Availability
Clone
9E2 (See other available formats)
Regulatory Status
RUO
Other Names
NKp46, NCR1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
9E2_PE-FIRE-810_CD56_Antibody-120222
Human peripheral blood lymphocytes stained with anti-human CD56 FITC and anti-human CD335 (clone 9E2) PE/Fire™ 810 (left) or stained with anti-human CD56 FITC only (right).
  • 9E2_PE-FIRE-810_CD56_Antibody-120222
    Human peripheral blood lymphocytes stained with anti-human CD56 FITC and anti-human CD335 (clone 9E2) PE/Fire™ 810 (left) or stained with anti-human CD56 FITC only (right).
Compare all formats See PE/Fire™ 810 spectral data
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331953 25 tests £174
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Description

CD335, also known as NKp46, is a member of the natural cytotoxicity receptor (NCR) family which triggers cytotoxicity in NK cells. CD335 is direct­ly involved in target cell recognition and lysis, and is exclusively expressed on CD3-CD56+ NK cells, suggesting it is a universal marker for NK cells. NKp46, along with NKp30 and NKp44, is referred to as a natural cytoxicity receptor (NCR) and plays a very important role in killing virus-infected tumor cells and MHC-class I-unprotected cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NKp46-Fc fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE/Fire™ 810 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

* PE/Fire™ 810 has a maximum excitation of 488/561 nm and a maximum emission of 810 nm.


Excessive exposure to light, and commonly used fixation, permeabilization buffers can affect PE/Fire™ 810 fluorescence signal intensity and spread. Please keep conjugates protected from light exposure. For more information and representative data, visit our Fire Dyes page.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone 9E2 has been shown to block NK activation through NKp46.6

Application References

(PubMed link indicates BioLegend citation)
  1. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
  2. Kalberer CP, et al. 2003. Blood 102:127.
  3. Chen Y, et al. 2007. J. Immunol. 179:2766.
  4. Jarahian M, et al. 2009. J. Virol. 83:8108. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Achdout H. et al. 2010. J. Virol. 84:3993.
RRID
AB_2936531 (BioLegend Cat. No. 331953)

Antigen Details

Structure
Type I membrane glycoprotein (46 kD)
Distribution

Expressed on resting and activated NK cells

Cell Type
NK cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Mandelboim O and Porgador A. 2001. Int. J. Biochem. Cell Biol. 33:1147.
2. Nakajima H, et al. 2000. Eur. J. Immunol. 30:3309.
3. Sivori S. 1999. Eur. J. Immunol. 29:1656.

Gene ID
9437 View all products for this Gene ID
UniProt
View information about CD335 on UniProt.org

Other Formats

View All CD335 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD335 (NKp46) 9E2 FC
Biotin anti-human CD335 (NKp46) 9E2 FC
PE anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 647 anti-human CD335 (NKp46) 9E2 FC
Pacific Blue™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 421™ anti-human CD335 (NKp46) 9E2 FC
PerCP/Cyanine5.5 anti-human CD335 (NKp46) 9E2 FC
APC anti-human CD335 (NKp46) 9E2 FC
PE/Cyanine7 anti-human CD335 (NKp46) 9E2 FC
FITC anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 510™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 605™ anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 650™ anti-human CD335 (NKp46) 9E2 FC
PE/Dazzle™ 594 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 700 anti-human CD335 (NKp46) 9E2 FC
Brilliant Violet 711™ anti-human CD335 (NKp46) 9E2 FC
APC/Fire™ 750 anti-human CD335 (NKp46) 9E2 FC
Alexa Fluor® 488 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-B0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-C0101 anti-human CD335 (NKp46) 9E2 PG
TotalSeq™-A0101 anti-human CD335 (NKp46) 9E2 PG
Brilliant Violet 785™ anti-human CD335 (NKp46) 9E2 FC
Ultra-LEAF™ Purified anti-human CD335 (NKp46) 9E2 FC,FA
APC/Cyanine7 anti-human CD335 (NKp46) Antibody 9E2 FC
PE/Cyanine5 anti-human CD335 (NKp46) 9E2 FC
PE/Fire™ 810 anti-human CD335 (NKp46) 9E2 FC
TotalSeq™-D0101 anti-human CD335 (NKp46) 9E2 PG
PE/Cyanine7 anti-human CD335 9E2 FC
Spark Red™ 718 anti-human CD335 (NKp46) (Flexi-Fluor™) 9E2 FC
Go To Top Version: 1    Revision Date: 12/02/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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