PE anti-SHIP-1 Antibody

Pricing & Availability
Clone
P1C1-A5 (See other available formats)
Regulatory Status
RUO
Other Names
Inositol Polyphosphate-5-Phosphatase, SIP-145, phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
P1C1-A5_PE_SHIP1_Antibody_FC_120213
C57BL/6 mouse bone marrow cells were stimulated with or without LPS for 48 hours, then fixed and permeabilized with BioLegend Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-1 (clone P1C1-A5) PE (filled histogram for LPS stimulated, open histogram with purple solid line for unstimulated) or mouse IgG1, κ PE isotype control (dashed line for unstimulated, dotted line for stimulated). Data shown was gated on the myeloid cell population.
  • P1C1-A5_PE_SHIP1_Antibody_FC_120213
    C57BL/6 mouse bone marrow cells were stimulated with or without LPS for 48 hours, then fixed and permeabilized with BioLegend Fixation Buffer (Cat. No. 420801) and Permeabilization Wash Buffer (Cat. No. 421002). Cells were then stained with SHIP-1 (clone P1C1-A5) PE (filled histogram for LPS stimulated, open histogram with purple solid line for unstimulated) or mouse IgG1, κ PE isotype control (dashed line for unstimulated, dotted line for stimulated). Data shown was gated on the myeloid cell population.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
656603 25 µg £99.00
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656604 100 µg £222.00
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Description

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes key signaling lipid PI(3,4,5)P(3) to PI(3,4)P(2). SHIP-1 is located in the cytoplasm and is recruited to the cell surface upon receptor ligation. It plays critical roles in hematopoietic cell proliferation, activation, differentiation, and survival by regulating PI3K and Akt pathways. Aberrant regulation of PI3K signaling can induce autoimmune diseases and cancer formation.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human SHIP fusion protein in CFA/IFA
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Specific to human SHIP-1 and cross reactive with mouse SHIP-1.

RRID
AB_2562867 (BioLegend Cat. No. 656603)
AB_2562868 (BioLegend Cat. No. 656604)

Antigen Details

Structure
1189 or 1188 amino acids, predicted molecular weight of 150 kD with the characteristic N-terminal SH2 domain, inositol phosphatase domain, and C-terminal protein interaction domains
Distribution
Cytoplasm and membrane; translocates to the plasma membrane when activated
Function
Negative regulator of hematopoietic cell proliferation and survival
Interaction
Phosphatidylinositol-3,4,5-triphosphate
Ligand/Receptor
SHC1, DOK1, DOK3, PTPN11/SHP-2, SLAMF1/CD150, PTPN11 FCGR2A FCGR2B, FCGR3, SRC and TEC et al.
Cell Type
B cells, Dendritic cells, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Immunology, Innate Immunity, Signal Transduction
Molecular Family
Protein Kinases/Phosphatase
Antigen References

1. Condé C, et al. 2011. Biochem. Pharmacol. 82:1320.
2. Parry RV, et al. 2010. Biochim Biophys Acta. 1804:592.
3. Ghansah T, et al. 2012. Oncoimmunology. 1:984.
4. Banh C, et al. 2012. Blood 120:4583.
5. Blunt MD, et al. 2012. Front. Immunol. 3:226.

Gene ID
3635 View all products for this Gene ID
UniProt
View information about SHIP-1 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 01/20/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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