PE anti-human/mouse CD3ε (activation epitope) Antibody

Pricing & Availability
Clone
APA1/1 (See other available formats)
Other Names
CD3e molecule, epsilon (CD3-TCR complex), CD3e antigen, CD3 epsilon
Isotype
Mouse IgG1, κ
Ave. Rating
2 reviews
Product Citations
publications
APA1-1_PE_CD3e_Antibody_FC_091014.jpg
Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3ε (clone APA1/1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • APA1-1_PE_CD3e_Antibody_FC_091014.jpg
    Human peripheral blood lymphocytes were fixed and permeabilized with 70% ethanol, followed by intracellular staining with CD3ε (clone APA1/1) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
See PE spectral data
Cat # Size Price Quantity Avail. Save
362703 25 tests £48
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362704 100 tests £144
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Description

The CD3ε chain of the CD3-TCR complex is a type I membrane protein that undergoes conformational change following ligation of the TCR-CD3 complex.  This conformational change leads to exposure of the proline rich sequence in CD3ε and results in recruitment of Nck. It is expressed by all mature T cells and forms a heteromeric complex with CD3 α/β or γ/δ and ζ chains. It is important in T cell development, and deficiency of this gene has been associated with severe immunodeficiency.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified denatured CD3 proteins from human thymus
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent intracellular staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Clone APA1/1 recognizes an activation-dependent intracellular epitope of the CD3ε chain. Additional reported applications (for the relevant formats of this clone) include: immunofluorescent staining of paraformaldehyde fixed sections1, in vivo tracking of T cells 2, and Western blotting3. The LEAF purified format is recommended for in vivo studies. 

Application References

(PubMed link indicates BioLegend citation)
  1. Salmerón A, et al. 1991. J. Immunol. 147:3047. (ICFC, IF)
  2. Risueño RM, et al. 2005. Blood. 106:601. (IF, in vivo tracking, Mouse Reactivity)
  3. Risueño RM, et al. 2008. PLos One. 3:e1747. (WB)
  4. Gil D, et al. 2005. J. Exp. Med. 201:517.
  5. Risueño RM, et al. 2006. Proc. Natl. Acad. Sci. USA. 103:9625.
RRID
AB_2564239 (BioLegend Cat. No. 362703)
AB_2564240 (BioLegend Cat. No. 362704)

Antigen Details

Structure
Type I membrane protein, part of the TCR-CD3 complex. 207 amino acids, predicted molecular weight of 23 kD.
Distribution
Mature T cells.
Function
Important role in T cell development and in linking antigen recognition with intracellular signaling pathways.
Interaction
Interacts with Nck.
Ligand/Receptor
MHC antigen.
Cell Type
T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Signal Transduction
Molecular Family
CD Molecules, TCRs
Antigen References

1. Savage PD, et al. 1988. Cytogenet. Cell. Genet. 49:289.
2. DeJarnette JB, et al. 1998. Proc. Natl. Acad. Sci USA. 95:14909.
3. Wang B, et al. 1999. J. Immunol. 162:88.
4. Szymczak AL, et al. 2005. J. Immunol. 175:270.
5. Borroto A, et al. 2014. J. Immunol. 192:2042.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3e on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/16/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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