PE anti-human CD69 Antibody

Pricing & Availability
Clone
FN50 (See other available formats)
Regulatory Status
RUO
Workshop
IV A91
Other Names
Very Early Activation Antigen (VEA), Activation inducer molecule (AIM)
Isotype
Mouse IgG1, κ
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Product Citations
publications
FN50
PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 PE
  • FN50
    PMA + ionomycin stimulated (6 hours) human lymphocytes stained with FN50 PE
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310905 25 tests £46
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310906 100 tests £98
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Description

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The CD69 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections2, immunofluorescence microscopy3, and spatial biology (IBEX)8,9.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp WB, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Sakkas LI, et al. 1998. Clin. and Diag. Lab. Immunol. 5:430. (IHC)
  3. Kim JR, et al. 2005. BMC Immunol. 6:3. (IF)
  4. Verjans GM, et al. 2007. P. Natl. Acad. Sci. USA 104:3496.
  5. Lu H, et al. 2009. Toxicol Sci. 112:363. (FC) PubMed
  6. Thakral D, et al. 2008. J. Immunol. 180:7431. (FC) PubMed
  7. Yoshino N, et al. 2000. Exp. Anim. (Tokyo) 49:97. (FC)
  8. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  9. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Kongsomboonvech AK, et al. 2020. PLoS Pathog. 16:e1008327. PubMed
  2. Soday L, et al. 2021. Frontiers in Immunology. 12:600056. PubMed
  3. Marquardt I, et al. 2022. Front Microbiol. 12:752549. PubMed
  4. Afolabi LO, et al. 2021. Front Immunol. 12:701671. PubMed
  5. Kagoya Y, et al. 2018. Nat Commun. 9:1915. PubMed
  6. Serra–Peinado C, et al. 2019. Nat Commun. 10:3705. PubMed
  7. Stras SF, et al. 2020. Developmental Cell. 51(3):357-373.e5.. PubMed
  8. Shen Y, et al. 2021. Comput Struct Biotechnol J. 19:5360. PubMed
  9. Magalhães L, et al. 2015. PLoS Negl Trop Dis. 10: 0003816. PubMed
  10. Kieffer T, et al. 2017. J Reprod Immunol. 10.1016/j.jri.2016.11.004. PubMed
  11. Fiaux PC, et al. 2020. PLoS Comput Biol. 16:e1008194. PubMed
  12. Schmitz T, et al. 2021. J Reprod Immunol. 148:103424. PubMed
  13. Thakral D, et al. 2008. J Immunol. 180:7431. PubMed
  14. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  15. Park S, et al. 2015. J Biol Chem. 290: 17349 - 17366. PubMed
  16. Stuart T, et al. 2019. Cell. 177:1888. PubMed
  17. Gardner TJ, et al. 2022. Nat Chem Biol. 18:216. PubMed
  18. Oikawa D, et al. 2020. Front Immunol. 11:601926. PubMed
  19. Lucas C, et al. 2021. Nature. Online ahead of print. PubMed
  20. Frensch M, et al. 2021. Cell Mol Life Sci. 78:8165. PubMed
  21. Sibener LV et al. 2018. Cell. 174(3):672-687 . PubMed
  22. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  23. Zhang Z, et al. 2021. Acta Pharm Sin B. 11:1965. PubMed
  24. Arvindam US, et al. 2021. Leukemia. 35:1586. PubMed
  25. Aschmoneit N, et al. 2022. Oncoimmunology. 11:2028961. PubMed
  26. Jaudszus A, et al. 2013. J Lipid Res. 54:923. PubMed
  27. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  28. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  29. Laroni A, et al. 2016. J Autoimmun. 72:8-18. PubMed
  30. Wallstabe L et al. 2019. JCI Insight. 4(18) pii: 126345. PubMed
  31. Aschmoneit N, et al. 2021. Sci Rep. 11:13880. PubMed
  32. Costantini C, et al. 2010. Int Immunol. 1.490972222. PubMed
  33. Halkias J, et al. 2019. J Clin Invest. 130:3562. PubMed
  34. Liu Y, et al. 2022. MAbs. 14:2073632. PubMed
  35. Feng Y, et al. 2022. Life (Basel). 12:. PubMed
  36. Li W, et al. 2020. Immunity. 53(2):456-470. PubMed
  37. Zhang P, et al. 2021. Nat Med. 22:. PubMed
  38. Tsai HI, et al. 2021. EMBO Mol Med. 13:e12834. PubMed
  39. Ng KW, et al. 2019. eLife. 0.333333333333333. PubMed
  40. Celis‐Gutierrez J et al. 2019. Cell Rep. 27(11):3315-3330 . PubMed
  41. Zhou Z, et al. 2022. J Gastrointest Oncol. 13:732. PubMed
  42. Tao L, et al. 2020. Cancers (Basel). 12:00. PubMed
  43. Su S, et al. 2016. Sci Rep. 6:20070. PubMed
  44. Zimmermann M, et al. 2019. Front Immunol. 10:829. PubMed
  45. Liu CF, et al. 2017. Front Immunol. 1.445833333. PubMed
  46. Shen C, et al. 2021. BMC Med. 19:283. PubMed
  47. Wang R, et al. 2016. Proc Natl Acad Sci U S A. 113: 11501 - 11506. PubMed
  48. Cohen Z, et al. 2020. Biomed Rep. 13:17. PubMed
  49. Luoma AM, et al. 2020. Cell. 182(3):655-671.e22. PubMed
  50. Guenther C, et al. 2019. Front Immunol. 10:1138. PubMed
  51. Shan L, et al. 2017. Immunity. 47:766. PubMed
  52. Hoseini SS, et al. 2021. Journal for ImmunoTherapy of Cancer. 9(5):. PubMed
RRID
AB_314840 (BioLegend Cat. No. 310905)
AB_314841 (BioLegend Cat. No. 310906)

Antigen Details

Structure
C-type lectin, type II glycoprotein, 28/32 kD
Distribution

Activated T cells, B cells, NK cells, granulocytes, thymocytes, platelets, Langerhans cells

Function
Lymphocyte, monocyte, and platelet activation, NK cell killing
Cell Type
B cells, Granulocytes, Langerhans cells, NK cells, Platelets, T cells, Thymocytes, Tregs
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Testi R, et al. 1994. Immunol. Today 15:479.

Gene ID
969 View all products for this Gene ID
UniProt
View information about CD69 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.

Other Formats

View All CD69 Reagents Request Custom Conjugation
Description Clone Applications
Purified anti-human CD69 FN50 FC,CyTOF®,IHC-F
FITC anti-human CD69 FN50 FC
PE anti-human CD69 FN50 FC
PE/Cyanine5 anti-human CD69 FN50 FC
APC anti-human CD69 FN50 FC
APC/Cyanine7 anti-human CD69 FN50 FC
PE/Cyanine7 anti-human CD69 FN50 FC
Alexa Fluor® 488 anti-human CD69 FN50 FC
Alexa Fluor® 647 anti-human CD69 FN50 FC,SB
Pacific Blue™ anti-human CD69 FN50 FC
Alexa Fluor® 700 anti-human CD69 FN50 FC
Biotin anti-human CD69 FN50 FC
PerCP/Cyanine5.5 anti-human CD69 FN50 FC
PerCP anti-human CD69 FN50 FC
Brilliant Violet 421™ anti-human CD69 FN50 FC
Brilliant Violet 785™ anti-human CD69 FN50 FC
Brilliant Violet 650™ anti-human CD69 FN50 FC
Brilliant Violet 510™ anti-human CD69 FN50 FC
Brilliant Violet 605™ anti-human CD69 FN50 FC
Purified anti-human CD69 (Maxpar® Ready) FN50 FC,CyTOF®
PE/Dazzle™ 594 anti-human CD69 FN50 FC
Brilliant Violet 711™ anti-human CD69 FN50 FC
APC/Fire™ 750 anti-human CD69 FN50 FC
TotalSeq™-A0146 anti-human CD69 FN50 PG
TotalSeq™-B0146 anti-human CD69 FN50 PG
TotalSeq™-C0146 anti-human CD69 FN50 PG
Brilliant Violet 750™ anti-human CD69 FN50 FC
KIRAVIA Blue 520™ anti-human CD69 FN50 FC
Spark NIR™ 685 anti-human CD69 Antibody FN50 FC
PE/Fire™ 640 anti-human CD69 FN50 FC
Spark YG™ 581 anti-human CD69 FN50 FC
TotalSeq™-D0146 anti-human CD69 FN50 PG
APC anti-human CD69 FN50 FC
Spark Blue™ 550 anti-human CD69 FN50 FC
PE anti-human CD69 FN50 FC
Spark Red™ 718 anti-human CD69 FN50 FC
GMP PE anti-human CD69 FN50 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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