Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
E13-161.7 (See other available formats)
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
E13-161dot7_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ E13-161.7
  • E13-161dot7_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ E13-161.7
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
122519 25 µg £57
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122520 100 µg £131
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Description

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™ .
Concentration
0.5 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

 

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  8. Hu W, et al. 2015 Blood. 125:2206. PubMed
Product Citations
  1. Grassinger J, et al. 2009. Blood. 114:49:00. PubMed
  2. Fossati V, et al. 2010. J Immunol. 184:1251. PubMed
  3. Ellis S, et al. 2011. Blood. 118:1516. PubMed
  4. Hu W, et al. 2015. Blood. 125:2206. PubMed
  5. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  6. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  7. Oben K, et al. 2017. PLoS One. 10.1371/journal.pone.0169767. PubMed
  8. Booth CAG 2018. Cancer cell. 33:274. PubMed
  9. Park SM 2018. Cell stem cell. 24:153. PubMed
  10. Jee D, et al. 2018. Mol Cell. 69:265. PubMed
  11. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  12. Sala D, et al. 2019. Nat Commun. 10:1796. PubMed
Publication Library
RRID
AB_2250602 (BioLegend Cat. No. 122519)
AB_2143237 (BioLegend Cat. No. 122520)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution
Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells
Function
Regulates B and T cell responses
Cell Type
Hematopoietic stem and progenitors, T cells, B cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 07/11/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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