Pacific Blue™ anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
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Product Citations
publications
M1slash70_Blue_091207
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram) (gated on total cells).
  • M1slash70_Blue_091207
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Pacific Blue™ (filled histogram) or rat IgG2b, κ Pacific Blue™ isotype control (open histogram) (gated on total cells).
See Pacific Blue™ spectral data
Cat # Size Price Quantity Avail. Save
101223 25 µg £58
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101224 100 µg £133
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The CD11b antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Buschor S, et al. 2017. PLoS Pathogens. 13(6):e1006476. PubMed
  2. Young JS, et al. 2018. Front Immunol. 9:1385. PubMed
  3. Fatkhullina AR et al. 2018. Immunity. 49(5):943-957 . PubMed
  4. Aurélien Trompette et al. 2018. Immunity. 48(5):992-1005 . PubMed
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  7. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  8. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  9. Hill RZ, et al. 2018. Elife. 7:e33285. PubMed
  10. Hong S et al. 2018. Immunity. 49(4):695-708 . PubMed
  11. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  12. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  13. Cao W, et al. 2017. Immunity. 47:1182. PubMed
  14. Zhu H, et al. 2018. Elife. 7:. PubMed
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  16. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  17. Ren S, et al. 2019. Oncol Rep. 41:1509. PubMed
  18. Bijalwan M, et al. 2019. Sci Rep. 9:3444. PubMed
  19. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  20. Matsumura T et al. 2019. Cell Rep. 27(2):561-571 . PubMed
  21. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  22. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  23. Uhl J, et al. 2019. J Cell Commun Signal. 13:209. PubMed
  24. Shen S, et al. 2018. Pain Med. 19:686. PubMed
  25. Schneider C, et al. 2018. Cell. 174:271. PubMed
  26. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  27. Hou X, et al. 2020. Cell Reports. 28(1):172-189.e7.. PubMed
  28. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  29. Dahlgren MW et al. 2019. Immunity. 50(3):707-722 . PubMed
  30. Arora H, et al. 2019. Immunity. 50:418. PubMed
  31. Yuan X, et al. 2017. Elife. 6:e29540. PubMed
  32. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  33. Leonard JD et al. 2017. Immunity. 47(1):107-117 . PubMed
  34. Kälin S et al. 2017. Cell metabolism. 26(3):475-492 . PubMed
  35. Jaitin DA et al. 2016. Cell. 167(7):1883-1896 . PubMed
  36. Tomlinson JE, et al. 2018. J Neuroinflammation. 0.753472222. PubMed
  37. Rothweiler S, et al. 2019. Purinergic Signal. 0.885416667. PubMed
  38. Yahagi A, et al. 2019. RMD Open. 5:e000853. PubMed
  39. Tomida S, et al. 2019. Sci Rep. 9:10751. PubMed
  40. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  41. Brenner E, et al. 2020. Nat Commun. 11:1335. PubMed
  42. Fang EF, et al. 2019. Nat Neurosci. 22:401. PubMed
  43. Si Y, et al. 2018. J Control Release. 282:120. PubMed
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RRID
AB_755985 (BioLegend Cat. No. 101223)
AB_755986 (BioLegend Cat. No. 101224)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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