HRP Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
405405 500 µl £81
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Aqueous buffered solution containing a carrier protein and preservatives.
Preparation
The polyclonal antibody was purified from goat antiserum by rat immunoglobulin affinity chromatography.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
This product is guaranteed for six months. The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

This product has a shelf-life of 12 months or less. Please use our Expiration Lookup Tool to verify the expiration date of your lot of product
Application

ELISA - Quality tested
IF, IHC, WB - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. Do not use this product beyond the expiration date. For ELISA or Western blot analysis, the reagent should be titrated between 1:1,000 - 1:5,000 to determine optimal conditions. For IHC on tissue sections, the reagent should be titrated between 1:500 - 1:2000 to determine optimal conditions. It is recommended that the reagent be titrated for optimal performance for each application. Avoid using diluent solutions containing sodium azide. Sodium azide is an inhibitor of horseradish peroxidase.

Application Notes

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Additional Product Notes

This HRP conjugated goat anti-rat IgG antibody is useful as a second step reagent for indirect immunohistochemistry staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
Product Citations
  1. Zuo X, et al. 2014. PLoS One. 9:84748. PubMed
  2. Sahlberg S, et al. 2014. PLoS One. 9:94621. PubMed
  3. Tuncel J, et al. 2016. PLoS One. 11: 0155936. PubMed
  4. Freidl R, et al. 2016. J Allergy Clin Immunol. S0091-6749(16)31348-3. PubMed
  5. Siddiqui S, et al. 2016. J Biol Chem. 292:1029-1037. PubMed
  6. Link E, et al. 2017. Vaccine. 10.1016/j.vaccine.2017.08.019. PubMed
  7. Kim DH, et al. 2018. Nat Commun. 9:503. PubMed
  8. van Tok MN, et al. 2018. Front Immunol. 9:1550. PubMed
  9. Mamedov T, et al. 2019. PLoS One. 14:e0213438. PubMed
Publication Library
RRID
AB_315016 (BioLegend Cat. No. 405405)

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For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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