Brilliant Violet 421™ Goat anti-rat IgG (minimal x-reactivity) Antibody

Pricing & Availability
Clone
Poly4054 (See other available formats)
Isotype
Goat Polyclonal IgG
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Product Citations
publications
Poly4054_BV421_081111
293E cells were stained with purified anti-Histone H3-Phospho (Ser28) antibody (clone HTA28) and labeled with BV421™ anti-rat IgG (clone Poly4054)(blue). Cytosol is indicated by Alexa Fluor® 488 anti-tubulin-alpha (clone 10D8)(green) and nuclei are stained by propidium iodide. HTA28 only stains mitotic cells (violet).
  • Poly4054_BV421_081111
    293E cells were stained with purified anti-Histone H3-Phospho (Ser28) antibody (clone HTA28) and labeled with BV421™ anti-rat IgG (clone Poly4054)(blue). Cytosol is indicated by Alexa Fluor® 488 anti-tubulin-alpha (clone 10D8)(green) and nuclei are stained by propidium iodide. HTA28 only stains mitotic cells (violet).
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
405414 125 µl £124
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Description

Goat anti-rat IgG antibody reacts primarily with the heavy chains of rat IgG, but also partially binds to the light chains common to most rat immunoglobulins. No cross-reactivity has been detected against non-immunoglobulin serum proteins. This antibody has been solid-phase absorbed to ensure minimal cross-reaction with rabbit, human, bovine, horse, and mouse immunoglobulins, but it may cross-react with other immunoglobulins from other species.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Rat
Antibody Type
Polyclonal
Host Species
Goat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IF - Quality tested
FC

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescence staining. For IF staining, the suggested dilution of this reagent is 1:20 - 1:50. It is recommended that the reagent be titrated for optimal performance for each application. 

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

This conjugated goat anti-rat IgG antibody is useful for direct immunofluorescent staining or as a second step reagent for indirect immunofluorescent staining when used in conjunction with primary antibodies.

Application References

(PubMed link indicates BioLegend citation)
  1. Zornetzer GA, et al. 2010. J. Virol. 84:11297. PubMed
  2. Frank NY, et al. 2011. Cancer Res. 71:1474. (IHC) PubMed
Product Citations
  1. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
Publication Library
RRID
AB_10900808 (BioLegend Cat. No. 405414)

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 2    Revision Date: 02/08/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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