FITC anti-human CD3 Antibody

Pricing & Availability
Clone
HIT3a (See other available formats)
Regulatory Status
RUO
Workshop
V CD03.05
Other Names
T3, CD3ε
Isotype
Mouse IgG2a, κ
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Product Citations
publications
HIT3a_FITC_073004
Human peripheral blood lymphocytes stained with HIT3a FITC
  • HIT3a_FITC_073004
    Human peripheral blood lymphocytes stained with HIT3a FITC
Compare all formats See FITC spectral data
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300305 25 tests £16
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300306 100 tests £38
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Description

CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported (for the relevant formats) applications include: immunohistochemical staining of acetone-fixed frozen sections, immunoprecipitation, and activation of T lymphocytes4-7. The HIT3a antibody is able to stimulate T cell activation. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 300314). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 300332) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
  3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
  4. Sedelies KA, et al. 2004. J. Biol. Chem. 279:26581. (Activ)
  5. Rivollier A, et al. 2004. Blood 104:4029. (Activ)
  6. Scharschmidt E, et al. 2004. Mol. Cell Biol. 24:3860. (Activ)
  7. Smeltz RB. 2007. J. Immunol. 178:4786. (Activ)
Product Citations
  1. Zhou Y, et al. 2020. Cancer Cell. 38(6):818-828.e5. PubMed
  2. Budde H, et al. 2017. Ann Hematol. 10.1007/s00277-017-2999-5. PubMed
  3. Rosa TLSA, et al. 2022. Front Med (Lausanne). 9:899998. PubMed
  4. Ding L, et al. 2021. Cancers (Basel). 13:. PubMed
  5. Delvecchio FR, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:1543. PubMed
  6. Parameswaran R, et al. 2016. Nat Commun. 7: 11154. PubMed
  7. Poran A, et al. 2020. Cell Reports Medicine. 1(8):100141. PubMed
  8. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  9. Jiang Q, et al. 2021. Cell Reports. 34(4):108670. PubMed
  10. Mondala PK, et al. 2021. Cell Stem Cell. 28(4):623-636.e9. PubMed
  11. Wang M, et al. 2022. Immun Inflamm Dis. 10:e626. PubMed
  12. Imbrechts M, et al. 2022. iScience. 25:104705. PubMed
  13. Sutton HJ, et al. 2021. Cell Reports. 34(6):108684. PubMed
  14. Bu S, et al. 2019. Int J Oncol. 55:179. PubMed
  15. Turner JS, et al. 2021. Nature. 596:109. PubMed
  16. Huang B, et al. 2020. Cell. 179(5):1160-1176.e24.. PubMed
  17. Stuart T, et al. 2019. Cell. 177:1888. PubMed
  18. Dean JW, et al. 2020. J Autoimmun. 108:102417. PubMed
  19. Hu W, et al. 2021. Vaccines (Basel). 9:. PubMed
  20. Sanchez-Vargas LA, et al. 2020. Viruses. 12:. PubMed
  21. Washburn ML, et al. 2019. J Immunol. 203:1897. PubMed
  22. Gingrich AA, et al. 2021. Front Immunol. 12:670309. PubMed
  23. Huang Y, et al. 2020. FASEB J. 34:1768. PubMed
  24. Wu H, et al. 2022. Biomolecules. 12:. PubMed
  25. Ott PA, et al. 2020. Cell. 183(2):347-362.e24. PubMed
  26. Pellegrini JM, et al. 2021. Sci Rep. 11:13559. PubMed
  27. Du Y, et al. 2022. Nat Commun. 13:231. PubMed
  28. Wang X, et al. 2021. Adv Sci (Weinh). 8:e2101447. PubMed
  29. Zhang J, et al. 2022. Nature. 609:369. PubMed
  30. Urlaub D, et al. 2019. Arthritis Res Ther. 1.067361111. PubMed
  31. Idorn M, et al. 2018. Oncoimmunology. 7:e1412029. PubMed
  32. Omer OS, et al. 2020. Methods Mol Biol. 2121:199. PubMed
  33. Abolhassani H, et al. 2017. J Exp Med . 214(1):91-106. PubMed
  34. Janela B, et al. 2019. Immunity. 50:1069. PubMed
  35. Guo S, et al. 2021. Stem Cells Int. 2021:9989790. PubMed
  36. Bourges C, et al. 2020. EMBO Mol Med. 12:e12112. PubMed
  37. Grant R, et al. 2021. Methods Protoc. 4:. PubMed
  38. Liu Y, et al. 2020. Oncol Lett. 2369:20. PubMed
  39. Linedale R, et al. 2017. PLoS One. 12:e0175755. PubMed
  40. Murdock BJ, et al. 2021. JCI Insight. 6:. PubMed
  41. Elias S, et al. 2020. Haematologica. . PubMed
  42. Motwani MP, et al. 2018. JCI Insight. 3:e94463. PubMed
  43. Jiang Q et al. 2019. Cancer cell. 35(1):81-94 . PubMed
  44. Liu B, et al. 2019. Mol Med Rep. 20:2823. PubMed
  45. Liu Y, et al. 2018. Cancer Cell. 33:480. PubMed
  46. Wu G, et al. 2021. Nat Cancer. 2:1170. PubMed
  47. Walle T, et al. 2022. Sci Adv. 8:eabh4050. PubMed
  48. Karlsson J, et al. 2020. Nat Commun. 1.773611111. PubMed
  49. Foote JR, et al. 2019. Front Immunol. 10:188. PubMed
  50. Warthan MD, et al. 2018. Biol Reprod. 98:309. PubMed
  51. Wadley AJ, et al. 2020. Brain Behav Immun Health. 3:100049. PubMed
  52. Dinh HQ, et al. 2020. Immunity. 53(2):319-334.e6. PubMed
  53. Gladkikh A, et al. 2017. Cancer Med. . 10.1002/cam4.1257. PubMed
  54. McGowan J, et al. 2019. Front Immunol. 10:1553. PubMed
  55. Yang JY, et al. 2020. J Cell Mol Med. . PubMed
  56. Forsberg EMV, et al. 2019. Cancer Res. 79:899. PubMed
RRID
AB_314041 (BioLegend Cat. No. 300305)
AB_314042 (BioLegend Cat. No. 300306)

Antigen Details

Structure
Ig superfamily, with the subunits of CD3γ, CD3δ, CD3ζ (CD247) and TCR (α/β or γ/δ) forms CD3/TCR complex, 20 kD
Distribution

Mature T and NK-T cells, thymocyte differentiation

Function
Antigen recognition, signal transduction, T cell activation
Ligand/Receptor
Peptide antigen bound to MHC
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507.

Gene ID
916 View all products for this Gene ID
UniProt
View information about CD3 on UniProt.org
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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