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Brilliant Violet 785™ anti-human CD33 Antibody

Pricing & Availability
Clone
WM53 (See other available formats)
Regulatory Status
RUO
Workshop
IV M-505
Other Names
Siglec-3, gp67, p67
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
WM53_BV785_CD33_Antibody_021317
Human peripheral blood monocytes were stained with Brilliant Violet 785™ anti-human CD33 (clone WM53) (filled histogram) or Brilliant Violet 785™ mouse IgG1, κ (open histogram).
  • WM53_BV785_CD33_Antibody_021317
    Human peripheral blood monocytes were stained with Brilliant Violet 785™ anti-human CD33 (clone WM53) (filled histogram) or Brilliant Violet 785™ mouse IgG1, κ (open histogram).
Compare all formats See Brilliant Violet 785™ spectral data
Cat # Size Price Quantity Check Availability Save
303427 25 tests £140
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303428 100 tests £298
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Description

CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human myeloid leukaemia cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 785™ under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 785™ excites at 405 nm and emits at 785 nm. The bandpass filter 780/60 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 785™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
  3. Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
  4. Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
Product Citations
  1. Hebbar N, et al. 2022. Nat Commun. 13:587. PubMed
  2. Holokai L, et al. 2020. Cancers (Basel). 12:00. PubMed
RRID
AB_2650887 (BioLegend Cat. No. 303427)
AB_2650888 (BioLegend Cat. No. 303428)

Antigen Details

Structure
Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
Distribution

Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells

Function
Adhesion and lectin activity
Ligand/Receptor
Sugar chains containing sialic acid
Cell Type
B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Siglec Molecules
Antigen References

1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005.

Gene ID
945 View all products for this Gene ID
UniProt
View information about CD33 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 10/30/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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