- Clone
- WM53 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- IV M-505
- Other Names
- Siglec-3, gp67, p67
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Human peripheral blood monocytes were stained with CD33 (clone WM53) Brilliant Violet 510™ (filled histogram) or mouse IgG1, κ Brilliant Violet 510™ isotype control (open histogram).
Cat # | Size | Price | Quantity Check Availability | Save | ||
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303421 | 25 tests | £133 | ||||
303422 | 100 tests | £291 |
CD33 is a 67 kD type I transmembrane glycoprotein also known as Siglec-3, gp67, and p67. It is a sialoadhesion immunoglobulin superfamily member expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. CD33 is absent on normal platelets, lymphocytes, erythrocytes and hematopoietic stem cells. CD33 functions as a sialic acid-dependent cell adhesion molecule with carbohydrate/lectin binding activity.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Chimpanzee
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human myeloid leukaemia cells.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 510™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents. - Excitation Laser
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Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation, Western blotting3, induction of cytokine production3, and immunofluorescence4. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 303437 & 303438).
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Application References
(PubMed link indicates BioLegend citation) -
- Knapp W, et al. 1989. Leucocyte Typing IV. Oxford University Press. New York.
- Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
- Garnache-Ottou F, et al. 2005. Blood 105:1256. (WB)
- Pèrez-Oliva AB, et al. 2011. Glycobiology. 21:757. (epitope, FC, IF)
- Product Citations
-
- RRID
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AB_2565657 (BioLegend Cat. No. 303421)
AB_2565658 (BioLegend Cat. No. 303422)
Antigen Details
- Structure
- Ig superfamily, sialoadhesins, type I glycoprotein, 67 kD
- Distribution
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Myeloid progenitor, monocytes, granulocytes, dendritic cells, mast cells
- Function
- Adhesion and lectin activity
- Ligand/Receptor
- Sugar chains containing sialic acid
- Cell Type
- B cells, Dendritic cells, Granulocytes, Hematopoietic stem and progenitors, Mast cells, Monocytes, Neutrophils
- Biology Area
- Cell Biology, Immunology, Neuroinflammation, Neuroscience
- Molecular Family
- CD Molecules, Siglec Molecules
- Antigen References
-
1. Favaloro E, et al. 1988. Br. J. Haematol. 69:163.
2. Freeman S, et al. 1995. Blood 85:2005. - Gene ID
- 945 View all products for this Gene ID
- UniProt
- View information about CD33 on UniProt.org
Related FAQs
Other Formats
View All CD33 Reagents Request Custom ConjugationCustomers Also Purchased


Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human CD33
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PE anti-human CD33
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PE/Cyanine5 anti-human CD33
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Purified anti-human CD33
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PerCP/Cyanine5.5 anti-human CD33
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Brilliant Violet 711™ anti-human CD33
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Brilliant Violet 421™ anti-human CD33
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Brilliant Violet 570™ anti-human CD33
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Purified anti-human CD33 (Maxpar® Ready)
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Brilliant Violet 510™ anti-human CD33
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Biotin anti-human CD33
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Brilliant Violet 785™ anti-human CD33
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Brilliant Violet 650™ anti-human CD33
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PE/Cyanine7 anti-human CD33
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PE/Dazzle™ 594 anti-human CD33
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APC anti-human CD33
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Alexa Fluor® 700 anti-human CD33
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Ultra-LEAF™ Purified anti-human CD33
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APC/Fire™ 750 anti-human CD33
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PerCP/Cyanine5.5 anti-human CD33
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APC/Cyanine7 anti-human CD33
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PE anti-human CD33
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PE/Cyanine7 anti-human CD33
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GMP PerCP/Cyanine5.5 anti-human CD33
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Alexa Fluor® 647 anti-human CD33
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GMP APC anti-human CD33
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GMP PE anti-human CD33
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PE/Fire™ 810 anti-human CD33
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PE/Cyanine5 anti-human CD33
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Spark Blue™ 515 anti-human CD33
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PE/Fire™ 700 anti-human CD33
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