Biotin anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
PK136_Biotin_1_110210
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_1_110210
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_2_110210
Cat # Size Price Quantity Avail. Save
108703 50 µg £27
Check Availability


Need larger quantities of this item?
Request Bulk Quote
108704 500 µg £82
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, and immunofluorescence microscopy11. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
Product Citations
  1. Garza-Rodea A, et al. 2011. PLoS One. 6:e14493. PubMed
  2. Zhu Z, et al. 2013. Proc Natl Acad Sci U S A. 110:1282. PubMed
  3. Uematsu T, et al. 2015. Sci Rep. 5: 17577. PubMed
  4. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  5. Becklund B, et al. 2016. Sci Rep. 6:30842. PubMed
  6. Galle-Treger L, et al. 2016. Nat Commun. 7:13202. PubMed
  7. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  8. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  9. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  10. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  11. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  12. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  13. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  14. Vasamsetti SB et al. 2018. Immunity. 49(1):93-106 . PubMed
  15. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  16. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  17. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  18. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  19. Yu VWC et al. 2017. Cell. 168(5):944-945 . PubMed
  20. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
Publication Library
RRID
AB_313390 (BioLegend Cat. No. 108703)
AB_313391 (BioLegend Cat. No. 108704)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 12/08/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login / Register
Forgot your password? Reset password?
Create an Account