Biotin anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_Biotin_1_110210
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_1_110210
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_2_110210
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108703 50 µg £33
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108704 500 µg £91
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  2. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Garza-Rodea A, et al. 2011. PLoS One. 6:e14493. PubMed
  5. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  6. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  7. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  8. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  9. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
  10. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  11. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  12. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  13. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
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  25. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  26. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  27. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  28. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  29. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  30. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  31. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  32. Shen Z, et al. 2021. iScience. 24:103014. PubMed
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  37. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
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  47. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
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RRID
AB_313390 (BioLegend Cat. No. 108703)
AB_313391 (BioLegend Cat. No. 108704)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 12/08/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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