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FITC anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136
C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) FITC and NKG2D PE.
  • PK136
    C57BL/6 mouse splenocytes were stained with NK1.1 (clone PK136) FITC and NKG2D PE.
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108705 50 µg £33
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108706 500 µg £91
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Petrova T, et al. 2020. Sci Rep. 10:3479. PubMed
  2. Zaman R, et al. 2021. Immunity. :. PubMed
  3. Gilleron J et al. 2018. Cell reports. 25(12):3329-3341 . PubMed
  4. Leier A, et al. 2022. Mol Ther Nucleic Acids. 28:261. PubMed
  5. Chung YM, et al. 2022. Am J Cancer Res. 12:1241. PubMed
  6. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  7. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  8. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  9. Liu X, et al. 2021. eLife. 0.416666666666667. PubMed
  10. Molina-Sanchez P, et al. 2020. Gastroenterology. 2203:159. PubMed
  11. Xu X, et al. 2021. J Nanobiotechnology. 19:376. PubMed
  12. Lebratti T, et al. 2021. eLife. 10:00. PubMed
  13. Enriquez AB, et al. 2022. iScience. 25:104305. PubMed
  14. Fullertona A, et al. 2013. Toxicol Appl Pharmacol. 266:317. PubMed
  15. Li X, et al. 2014. PLoS One. 9:108192. PubMed
  16. Marchingo JM, et al. 2020. eLife. 9:e53725.. PubMed
  17. Sodji QH, et al. 2022. Cancer Res Commun. 2:725. PubMed
  18. Zhang B, et al. 2021. Nat Biomed Eng. 5:1288. PubMed
  19. Lissner MM, et al. 2020. Elife. 9:00. PubMed
  20. Köchl R, et al. 2020. Elife. 9:00. PubMed
  21. Song Y, et al. 2016. Mol Cancer Ther. 15: 2413 - 2421. PubMed
  22. Kotfis K, et al. 2020. British Journal of Pharmacology. 49(1):66-72. PubMed
  23. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  24. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  25. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  26. Pardy RD, et al. 2021. Nat Commun. 12:4051. PubMed
  27. Lu X, et al. 2016. Nat Commun. 7: 12719. PubMed
  28. Hrdinka M, et al. 2016. PLoS One. 11: 0162863. PubMed
  29. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  30. Niemann J, et al. 2019. Nat Commun. 10:3236. PubMed
  31. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  32. Chung YM, et al. 2021. J Immunother Cancer. 9:. PubMed
  33. Garo LP, et al. 2019. Cell Rep. 28:3353. PubMed
  34. Calcinotto A, et al. 2018. Nat Commun. 9:4832. PubMed
  35. QT N, et al. 2015. Antimicrob Agents Chemother. 59: 6308-6316. PubMed
  36. Wolf KG, et al. 2022. JVS Vasc Sci. 3:336. PubMed
  37. Ballesteros I, et al. 2020. Cell. 183(5):1282-1297.e18. PubMed
  38. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  39. Giampazolias E, et al. 2021. Cell. . PubMed
  40. Robinett RA et al. 2018. Cell systems. 7(1):41-48 . PubMed
  41. Shahzad KA, et al. 2018. Drug Deliv. 25:703. PubMed
  42. Zhang W, et al. 2019. Mar Drugs. 0.845138889. PubMed
  43. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  44. Bronisz–Budzynska I, et al. 2019. Skelet Muscle. 9:22. PubMed
  45. Lam KC, et al. 2021. Cell. 184:5338. PubMed
  46. Ruscher R, et al. 2018. Bio Protoc. 8:e2757. PubMed
  47. Nam J, et al. 2021. Adv Ther (Weinh). 4:. PubMed
  48. Damgaard RB et al. 2016. Cell. 166(5):1215-1230 . PubMed
  49. Valkenburg S, et al. 2016. Sci Rep. 6:22666. PubMed
  50. Yuan C, et al. 2015. Biochem Biophys Res Commun. 464: 249-255. PubMed
  51. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  52. Werner A, et al. 2021. iScience. 24:103076. PubMed
  53. Schaffert S, et al. 2015. J Immunol. 195: 1470-1479. PubMed
  54. Jia H, et al. 2018. Int J Oncol. 53:949. PubMed
  55. Bárcena C et al. 2019. EBioMedicine. 43:513-524 . PubMed
  56. Zegarra‐Ruiz DF et al. 2018. Cell host & microbe. 25(1):113-127 . PubMed
  57. Wang X, et al. 2022. Elife. 11:. PubMed
  58. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  59. Iwanami N, et al. 2020. iScience. 23:101260. PubMed
  60. Chen J, et al. 2014. Cell Res. 24:1050. PubMed
  61. Goldberg EL, et al. 2021. Cell Metabolism. :. PubMed
  62. Krotova K, et al. 2019. Mol Ther Oncolytics. 15:166. PubMed
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  64. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
RRID
AB_313392 (BioLegend Cat. No. 108705)
AB_313393 (BioLegend Cat. No. 108706)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 12/08/2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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