- 3C10 (See other available formats)
- Regulatory Status
- Other Names
- T cell antigen receptor Vα7.2
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
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The 3C10 antibody recognizes the Vα7.2 T cell antigen receptor (TCR) α-chain segment which, joined with the Jα33 segment, constitutes an invariant TCR that is a characteristic of the mucosal-associated invariant T cells (MAIT cells). MAIT cells are restricted by a nonpolymorphic class Ib major histocompatibility complex (MHC) molecule, MHC-related molecule 1 (MR1). MAIT cells are present in human blood (1-8% of T cells), mesenteric lymph nodes, liver, and intestinal mucosa. MAIT cells play a role in detecting and fighting off microbial infections.Product Details
- Antibody Type
- Host Species
- Recombinant TCR
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Associated with an anti-CD161 or -IL18Ra staining, the 3C10 antibody allows unequivocal identification of MAIT cells. Importantly, the Va7.2 segment can also be used by conventional T cells. Therefore, the 3C10 also stains a subset of conventional CD4 and CD8 T cells.
(PubMed link indicates BioLegend citation)
- Martin E, et al. 2009. PLoS Biol. 7:525.
- Wakao H, et al. 2013. Cell Stem Cell 12:1. PubMed
- Product Citations
AB_2566057 (BioLegend Cat. No. 351724)
- Invariant T cell antigen receptor (TCR) α-chain (iTCRa), composed of the invariant α-chain variable region 7.2 (iVα7.2) segment in humans
Mucosal-associated invariant T (MAIT) cells and conventional T cells
- Cell Type
- T cells
- Biology Area
- Immunology, Adaptive Immunity
- Molecular Family
- Antigen References
1. Le Bourhis L, et al. 2010. Nat. Immunol. 11:701.
- Gene ID
- 6955 View all products for this Gene ID
- View information about TCR V alpha7.2 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
|PerCP/Cyanine5.5 anti-human TCR Vα7.2||3C10||FC|
|PE/Cyanine7 anti-human TCR Vα7.2||3C10||FC|
|APC/Cyanine7 anti-human TCR Vα7.2||3C10||FC|
|Brilliant Violet 421™ anti-human TCR Vα7.2||3C10||FC|
|Brilliant Violet 510™ anti-human TCR Vα7.2||3C10||FC|
|Brilliant Violet 711™ anti-human TCR Vα7.2||3C10||FC|
|TotalSeq™-C0581 anti-human TCR Vα7.2||3C10||PG|
|TotalSeq™-B0581 anti-human TCR Vα7.2||3C10||PG|
|Purified anti-human TCR Vα7.2||3C10||FC|
|FITC anti-human TCR Vα7.2||3C10||FC|
|PE anti-human TCR Vα7.2||3C10||FC|
|Alexa Fluor® 647 anti-human TCR Vα7.2||3C10||FC|
|Alexa Fluor® 700 anti-human TCR Vα7.2||3C10||FC|
|PE/Dazzle™ 594 anti-human TCR Vα7.2||3C10||FC|
|Brilliant Violet 605™ anti-human TCR Vα7.2||3C10||FC|
|TotalSeq™-A0581 anti-human TCR Vα7.2||3C10||PG|
|APC anti-human TCR Vα7.2||3C10||FC|
|Brilliant Violet 785™ anti-human TCR Vα7.2||3C10||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
PerCP/Cyanine5.5 anti-human TCR Vα7.2
PE/Cyanine7 anti-human TCR Vα7.2
APC/Cyanine7 anti-human TCR Vα7.2
Brilliant Violet 421™ anti-human TCR Vα7.2
Brilliant Violet 510™ anti-human TCR Vα7.2
Brilliant Violet 711™ anti-human TCR Vα7.2
TotalSeq™-C0581 anti-human TCR Vα7.2
TotalSeq™-B0581 anti-human TCR Vα7.2
Purified anti-human TCR Vα7.2
FITC anti-human TCR Vα7.2
PE anti-human TCR Vα7.2
Alexa Fluor® 647 anti-human TCR Vα7.2
Alexa Fluor® 700 anti-human TCR Vα7.2
PE/Dazzle™ 594 anti-human TCR Vα7.2
Brilliant Violet 605™ anti-human TCR Vα7.2
TotalSeq™-A0581 anti-human TCR Vα7.2
APC anti-human TCR Vα7.2
Brilliant Violet 785™ anti-human TCR Vα7.2