Biotin anti-Apo E Antibody

Pricing & Availability
Clone
D6E10 (See other available formats)
Other Names
Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
Isotype
Mouse IgG1
Ave. Rating
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Product Citations
publications
D6E10_Biotin_Apo-E_Antibody_100818
Western blot of Biotin anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant human protein; Lane 3: 40 ng of Apo E3 recombinant human protein; Lane 4: 40 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
  • D6E10_Biotin_Apo-E_Antibody_100818
    Western blot of Biotin anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 40 ng of Apo E2 recombinant human protein; Lane 3: 40 ng of Apo E3 recombinant human protein; Lane 4: 40 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60 minutes at room temperature. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # Size Price Quantity Avail. Save
803305 25 µg £74
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803306 100 µg £171
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Description

Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

Application References

(PubMed link indicates BioLegend citation)
  1. Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
  2. Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
  3. Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
  4. Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
  5. Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
  6. Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
  7. Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
  8. Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
RRID
AB_2783377 (BioLegend Cat. No. 803305)
AB_2783378 (BioLegend Cat. No. 803306)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
Gene ID
348 View all products for this Gene ID
UniProt
View information about Apo E on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 10/09/2018

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