- Clone
- D6E10 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Apolipoprotein E, apolipoprotein E3, AD2, LDLCQ5, LPG, ApoE
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications

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Western blot of HRP anti-Apo E antibody (clone D6E10). Lane 1: Molecular weight marker; Lane 2: 50 ng of Apo E2 recombinant human protein; Lane 3: 50 ng of Apo E3 recombinant human protein; Lane 4: 50 ng of Apo E4 recombinant human protein; Lane 5: 40 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody at 4°C overnight. Enhanced chemiluminescence (Cat. No. 426302) was used as the detection system.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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803308 | 100 µg | £213 |
Apo-E is a class of apolipoprotein primarily synthesized in the liver, but is also found in other tissues such as brain, kidneys, and spleen. Apo-E is a protein component of serum chylomicron and lipoprotein particles, playing essential roles in cholesterol transportation, homeostasis, and metabolism. The expression of Apo-E is induced by cholesterol-rich diets and hyperlipoproteinemia. In the central nervous system, Apo-E is produced by astrocytes and microglia. The Apo-E-containing lipoprotein particles play important functions in brain injury repair, neurogenesis, and synaptic plasticity. Apo-E has been found to be involved in the regulation of amyloid-β aggregation and clearance. Genetic polymorphisms of Apo-E are associated with neuron degenerative disorders including Alzheimer's disease.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was raised against a peptide sequence corresponding to aa 141-160 of human Apo-E.
- Formulation
- This antibody is provided in 50% glycerol in aqueous buffered solutions with preservatives.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with HRP under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, the antibody solution should be stored undiluted at -20°C, and protected from prolonged exposure to light.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB).
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Application References
(PubMed link indicates BioLegend citation) -
- Munoz DG and Feldman H. 2000. CMAJ. 162(1):65-72.
- Hagberg JM, et al. 2000. Physiol Genomics. 4(2):101-108.
- Saunders AM. 2000. J Neuropathol Exp Neurol. 59(9):751-8.
- Golabek AA, et al. 2000. Biophys J. 79(2):1008-15.
- Zunarelli E, et al. 2000. Breast Cancer Res Treat. 63(3):193-8.
- Zunarelli E, et al. 2000. Clin Neuropathol. 19(1):1-6.
- Tomiyama T, et al. 1999. Cell Mol Life Sci. 6(3-4):268-79.
- Golabek AA., et al. 1996. J Biol Chem. 271(18):10602-6.
- RRID
-
AB_2783380 (BioLegend Cat. No. 803308)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Gene ID
- 348 View all products for this Gene ID
- UniProt
- View information about Apo E on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All Apo E Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Apo E | D6E10 | WB,IHC-P |
Biotin anti-Apo E | D6E10 | WB |
HRP anti-Apo E | D6E10 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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