APC/Fire™ 750 anti-human CD49a Antibody

Pricing & Availability
Clone
TS2/7 (See other available formats)
Other Names
α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS2-7_APCFire750_CD49a_Antibody_050119
Human cervical cancer cell line, HeLa, was stained with CD49a (clone TS2/7) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
  • TS2-7_APCFire750_CD49a_Antibody_050119
    Human cervical cancer cell line, HeLa, was stained with CD49a (clone TS2/7) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
328317 25 tests £74
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328318 100 tests £178
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Description

CD49a is a 200 kD type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or Integrin α1. It associates with CD29 (β1 integrin) to form VLA-1 complex, a collagen IV and alminin-1 receptor. It is expressed on activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CTL line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications include: immunoprecipitation (1) and immunohistochemical staining (1) of acetone-fixed frozen tissue sections

Application References

(PubMed link indicates BioLegend citation)
  1. Hemler ME, et al. 1984. J.Immunol. 132:3011
  2. Hemler ME, et al. 1985. J. Biol. Chem. 260:15246

Antigen Details

Structure
Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
Distribution

Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells

Function
Adhesion, leukocyte migration
Ligand/Receptor
With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
Cell Type
Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
  2. Boiret N, et al. 2005. Exp. Hematol. 33:219
Gene ID
3672 View all products for this Gene ID
UniProt
View information about CD49a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 05/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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