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FITC anti-human CD49a Antibody

Pricing & Availability
Clone
TS2/7 (See other available formats)
Regulatory Status
RUO
Other Names
α1 integrin, VLA-1 α chain, Integrin α1 chain, ITGA1
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TS2slash7_FITC_082808.jpg
Human cervical cancer cell line, Hela, stained with TS2/7 FITC
  • TS2slash7_FITC_082808.jpg
    Human cervical cancer cell line, Hela, stained with TS2/7 FITC
Compare all formats See FITC spectral data
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328307 25 tests £65
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328308 100 tests £145
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Description

CD49a is a 200 kD type I transmembrane glycoprotein also known as α1 integrin, VLA-1 α chain, or Integrin α1. It associates with CD29 (β1 integrin) to form VLA-1 complex, a collagen IV and alminin-1 receptor. It is expressed on activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells. CD49a is an adhesion molecule and is involved in the regulation of leukocyte migration, T cell proliferation, and cytokine production.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CTL line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with FITC under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications include: immunoprecipitation1, immunohistochemical staining1 of acetone-fixed frozen tissue sections, and spatial biology (IBEX)3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hemler ME, et al. 1984. J.Immunol. 132:3011
  2. Hemler ME, et al. 1985. J. Biol. Chem. 260:15246
  3. Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
  4. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Stegmann K, et al. 2016. Sci Rep. 6: 26157. PubMed
  2. Martrus G, et al. 2017. PLoS One.. 10.1371/journal.pone.0182532. PubMed
  3. Shlamkovich T, et al. 2020. BMC Biology. 16(1):92. PubMed
RRID
AB_1236430 (BioLegend Cat. No. 328307)
AB_2129084 (BioLegend Cat. No. 328308)

Antigen Details

Structure
Integrin alpha chain family, Type I membrane protein, alpha chain of heterodimeric integrin receptor, 200 kD. Associates with CD29 to form VLA-1 complex
Distribution

Activated T cells, monocytes, NK cells, smooth muscle cells, neuronal cells, fibroblasts, and mesenchymal cells

Function
Adhesion, leukocyte migration
Ligand/Receptor
With integrin β1 (CD29) forms receptor for laminin-1 and collagen IV
Cell Type
Fibroblasts, Mesenchymal cells, Mesenchymal Stem Cells, Monocytes, NK cells, T cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Zola H, et al. Eds. 2007. Leukocyte and Stromal Cell Molecules:The CD Markers. Wiley-Liss Press. p122
  2. Boiret N, et al. 2005. Exp. Hematol. 33:219
Gene ID
3672 View all products for this Gene ID
UniProt
View information about CD49a on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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