APC anti-mouse CD48 Antibody

Pricing & Availability
Clone
HM48-1 (See other available formats)
Other Names
BCM1, SLAMF2, Blast-1
Isotype
Armenian Hamster IgG
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Product Citations
publications
HM48-1_APC_090507
C57BL/6 mouse splenocytes stained with HM48-1 APC
  • HM48-1_APC_090507
    C57BL/6 mouse splenocytes stained with HM48-1 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
103411 25 µg £58
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103412 100 µg £181
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Description

CD48 is a 45 kD GPI-anchored glycoprotein also known as BCM1, Blast-1 (human), and OX-45 (rat). It is a member of the Ig superfamily, expressed on T and B cells and monocytes/macrophages. It plays a role in adhesion and T cell recognition. The primary ligands for CD48 are CD2 and CD244.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse T lymphoma MBL-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM48-1 antibody is useful for blocking in vitro and in vivo CD48 mediated interactions. Additional reported applications (for the relevant formats) include: immunoprecipitation1, costimulation of T cell proliferation1,2, blocking of CD48-CD2 interaction1, and inhibition of CTL activity and graft rejection1,2. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. Nos. 103452-103456) with a lower endotoxin limit (Endotoxin < 0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Kato K, et al. 1992. J. Exp. Med. 176:1241. (IP, Costim, Block)
  2. Qin L, et al. 1994. J. Exp. Med. 179:341. (Costim, Block)
Product Citations
  1. Fontinha D, et al. 2015. PLoS One. 10: 0142540. PubMed
  2. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  3. Zhang Y, et al. 2016. Sci Rep. 6:37827. PubMed
  4. Grinenko T, et al. 2018. Nat Commun. 9:1898. PubMed
  5. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  6. Stivala S, et al. 2019. J Clin Invest. 130:1596. PubMed
  7. Bae J, et al. 2019. Nat Commun. 10:3496. PubMed
  8. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  9. Radulović V, et al. 2019. Cell Rep. 27:2826. PubMed
  10. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  11. Comazzetto S et al. 2018. Cell stem cell. 24(3):477-486 . PubMed
  12. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  13. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  14. Maetzig T, et al. 2017. Mol Ther. 25:606. PubMed
  15. Di Genua C, et al. 2019. Haematologica. 104:2215. PubMed
  16. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  17. Hidalgo San Jose L, et al. 2020. Cell Rep. 30:69. PubMed
RRID
AB_571996 (BioLegend Cat. No. 103411)
AB_571997 (BioLegend Cat. No. 103412)

Antigen Details

Structure
Ig superfamily, 45 kD
Distribution

T cells and B cells, monocytes/macrophages

Function
Adhesion, T cell recognition
Ligand/Receptor
CD2, CD244
Cell Type
B cells, Macrophages, Monocytes, T cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Flament C, et al. 1996. Hum. Immunol. 46:82.
3. Van der Merwe PA, et al. 1995. Curr. Biol. 5:74.
4. Latchman Y, et al. 1998. J. Immunol. 161:5809.

Gene ID
12506 View all products for this Gene ID
UniProt
View information about CD48 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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