APC anti-mouse CD279 (PD-1) Antibody

Pricing & Availability
Clone
RMP1-30 (See other available formats)
Regulatory Status
RUO
Other Names
Programmed Death-1, CD279, PDCD1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
RMP1-30_APC_062310
Con A-stimulated C57BL/6 splenocytes (Day 3) stained with RMP1-30 APC
  • RMP1-30_APC_062310
    Con A-stimulated C57BL/6 splenocytes (Day 3) stained with RMP1-30 APC
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Cat # Size Price Quantity Check Availability Save
109111 25 µg £68
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109112 100 µg £208
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Description

CD279 is a 50-55 kD immunoglobulin superfamily member also known as programmed death-1 (PD-1). PD-1 is expressed on a subset of CD4-CD8- thymocytes and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PD-1 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RMP1-30 antibody does not block the binding of PD-1 to B7-H1 and B7-DC1.

Application References

(PubMed link indicates BioLegend citation)
  1. Matsumoto K, et al. 2004. J. Immunol. 172:2530.
  2. Raimondi G, et al. 2006. J. Immunol. 176:2808. (FC) PubMed
  3. King IL, et al. 2009. J. Exp Med 206:1001. (FC) PubMed
Product Citations
  1. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  2. Valencia–Hernandez AM, et al. 2020. Cell Host & Microbe. 27(6):950-962. PubMed
  3. Tatsuguchi T, et al. 2022. Int Immunol. 34:277. PubMed
  4. Manukian G, et al. 2021. Int J Radiat Oncol Biol Phys. 110:1341. PubMed
  5. Carlsson J, et al. 2015. PLoS One. 10: 0143741. PubMed
  6. Zhang Y, et al. 2020. Oncol Lett. 1.053472222. PubMed
  7. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  8. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  9. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  10. Shen Y, et al. 2022. CNS Neurosci Ther. 28:46. PubMed
  11. Lv Z, et al. 2020. Oncoimmunology. 9:1747688. PubMed
  12. Sekiya T, et al. 2015. J Exp Med. 212: 1623-1640. PubMed
  13. Chauveau L, et al. 2021. EMBO Rep. 22:e52447. PubMed
  14. Xing Y, et al. 2022. Cancer Lett. 529:100. PubMed
  15. Cedeno-Laurent F, et al. 2010. J Immunol. 185:4659. PubMed
  16. Zhao Y et al. 2018. Cell reports. 24(2):379-390 . PubMed
  17. Kato Y, et al. 2015. J Immunol. 195: 1006-1014. PubMed
  18. Lewis KE, et al. 2018. Oncoimmunology. 7:e1377873. PubMed
  19. Zhong M, et al. 2022. Cell Death Dis. 13:671. PubMed
  20. Wang X, et al. 2021. Theranostics. 11:209. PubMed
  21. Laczkó D, et al. 2020. Immunity. 53:724. PubMed
RRID
AB_10613470 (BioLegend Cat. No. 109111)
AB_10612938 (BioLegend Cat. No. 109112)

Antigen Details

Structure
Ig superfamily, 50-55 kD
Distribution

Subset of double negative thymocytes, activated T and B cells

Function
Lymphocyte clonal selection, peripheral tolerance
Ligand/Receptor
PD-L1 (B7-H1), PD-L2
Cell Type
B cells, T cells, Thymocytes, Tregs
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Agata Y, et al. 1996. Int. Immunol. 8:765.
3. Nishimura H, et al. 2001. Science 291:319.
4. Ishida Y, et al. 1992. EMBO J. 11:3887.

Gene ID
18566 View all products for this Gene ID
UniProt
View information about CD279 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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