APC anti-human CD72 Antibody

Pricing & Availability
Clone
3F3 (See other available formats)
Workshop
VI CD72.1
Other Names
Lyb-2, Ly-19.2, Ly-32.2
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
3F3_APC_CD72_Antibody_100419
Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human CD72 (clone 3F3) APC (left) or mouse IgG2b, κ APC isotype control (right).
  • 3F3_APC_CD72_Antibody_100419
    Human peripheral blood lymphocytes were stained with CD19 FITC and anti-human CD72 (clone 3F3) APC (left) or mouse IgG2b, κ APC isotype control (right).
See APC spectral data
Cat # Size Price Quantity Avail. Save
316209 25 tests £74
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316210 100 tests £218
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Description

CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human lymphocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation.

Application References

(PubMed link indicates BioLegend citation)
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.

Antigen Details

Structure
Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Distribution

Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells

Function
negative regulation of B cell receptor signaling
Ligand/Receptor
CD5, CD100/Sema4D
Cell Type
B cells, Dendritic cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References
  1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  2. Schwarting T, et al. 1992. Am. J. Hematol. 41:151.
  3. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155.
  4. Kumanogoh A, et al. 2000. Immunity 13:621.
  5. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75.
  6. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
Gene ID
971 View all products for this Gene ID
UniProt
View information about CD72 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 10/04/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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