- 3F3 (See other available formats)
- VI CD72.1
- Other Names
- Lyb-2, Ly-19.2, Ly-32.2
- Mouse IgG2b, κ
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- Product Citations
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CD72 is a 39-43 kD type II membrane glycoprotein. It is a disulfide-linked homodimer belonging to C-type lectin family. CD72 is a pan-B cell marker expressed on pre-pre-B cells throughout B cell differentiation with the exception of plasma cells. It is also expressed on follicular dendritic cells, splenic red pulp macrophages (but not on peripheral blood monocytes), and liver Kupffer cells. CD72, a negative coreceptor of B cells, contains immunoreceptor tyrosine-based inhibitory motifs in the cytoplasmic domain which has been shown to recruit the tyrosine phosphatase SHP-1. Ligation of CD72 with its ligand regulates CD72 tyrosine dephosphorylation and SHP-1 dissociation to promote B cell activation and proliferation. CD100 and CD5 have been shown to be CD72 ligands. The CD100-CD72 interaction plays a role in maintenance of B cell homeostasis.Product Details
- Antibody Type
- Host Species
- Human lymphocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
- The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions. The solution is free of unconjugated DNA and unconjugated antibody.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
PG - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. For Proteogenomics TotalSeq™-A analysis, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
To maximize performance, centrifuge the antibody dilution (1.0 µg of antibody in 100 µl of staining buffer for every 1 million cells) before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation.
- Additional Product Notes
TotalSeq™ reagents are designed to profile protein levels at a single cell level following an optimized protocol similar to the CITE-seq workflow. A compatible single cell device (e.g. 10x Genomics Chromium System and Reagents) and sequencer (e.g Illumina analyzers) are required. Please contact technical support for more information, or visit biolegend.com/totalseq.
The TotalSeq™-A barcode sequence associated with clone 3F3 is CAGTCGTGGTAGATA.
The flanking sequences are CCTTGGCACCCGAGAATTCCA, and the poly A tail, BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A, where B represents either C, G, or T, and * indicates a phosphorothioated bond, to prevent nuclease degradation.
The full oligomer sequence for this product, with the specific barcode in brackets is CCTTGGCACCCGAGAATTCCA [CAGTCGTGGTAGATA]BAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA*A*A.
(PubMed link indicates BioLegend citation)
- Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
AB_2783189 (BioLegend Cat. No. 316205)
- Type II membrane glycoprotein, C-type lectin family, 39-43 kD, homodimer
Pre-pre-B cells throughout B cells differentiation (but not on plasma cells), follicular dendritic cells, splenic red pulp macrophages, liver Kupffer cells
- negative regulation of B cell receptor signaling
- Ligand Receptor
- CD5, CD100/Sema4D
- Cell Type
- B cells, Dendritic cells
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
2. Schwarting T, et al. 1992. Am. J. Hematol. 41:151.
3. Wu HJ and S. Bondata. 2002. Immunol. Res. 25:155.
4. Kumanogoh A, et al. 2000. Immunity 13:621.
5. Parnes JR and C. Pan. 2000. Immunol. Rev. 176:75.
6. Kumanogoh A, et al. 2005. Intnl. Immunol. 17:1277.
- Gene ID
- 971 View all products for this Gene ID
- View information about CD72 on UniProt.org
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