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Alexa Fluor® 647 Mouse IgG1, κ Isotype Ctrl (FC) Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
400130 100 tests £120
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The IgG1,κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, use the isotype control at the same concentration as your primary antibody. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Application References

(PubMed link indicates BioLegend citation)
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  2. Baxter AE, et al. 2017. Nat Protoc. 12:2029. PubMed
  3. de Tymowski C et al. 2019. Cell reports. 27(3):762-775 . PubMed
  4. Koyama M et al. 2019. Immunity. 51(5):885-898 . PubMed
  5. Hsu AY, et al. 2019. Mol Immunol. 112:206. PubMed
  6. Huang N, et al. 2020. Genome Biol. 1.03125. PubMed
  7. Li C, et al. 2014. Cell Signal. 26:1369. PubMed
  8. Zhou W, et al. 2020. J Cell Sci. 133:00:00. PubMed
  9. Meylan P, et al. 2021. Scientific Reports. 11(1):7847. PubMed
RRID
AB_2800436 (BioLegend Cat. No. 400130)

Related FAQs

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Go To Top Version: 4    Revision Date: 02/22/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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