PE Mouse IgG1, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
MOPC-21 (See other available formats)
Regulatory Status
RUO
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
400111 25 µg £21.00
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400112 100 µg £48.00
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Description

The MOPC-21 immunoglobulin has unknown specificity. The isotype of this antibody is mouse IgG1, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical Data Sheet (pdf)

Product Details

Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The immunoglobulin was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The IgG1, κ isotype control solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this mouse IgG1, κ isotype control antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest. Use our Concentration Lookup tool to find the exact concentrations of your lots of product.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), Functional Assay (FA)

Application References

(PubMed link indicates BioLegend citation)
  1. Carlsten M, et al. 2007. Cancer Res. 67:1317. PubMed
  2. Smed-Sörensen A, et al. 2008. Blood 111:5037. PubMed (FA)
  3. Bunesmann MM, et al. 2011. Am. J. Respir. Cell. Mol. Biol. Epub. PubMed
  4. Matsuyama T, et al. 2005. Infect. Immun. 73:1044. (IF)
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
  6. Lian IA, et al. 2011. Placenta. 32:823. PubMed
  7. Bufe B, et al. 20015. J Biol Chem. 290:7369. PubMed
Product Citations
  1. Wang F, et al. 2018. Oncogene. 37:286. PubMed
  2. Zhang HC, et al. 2018. Int J Mol Med. 41:13. PubMed
  3. Nam GH, et al. 2018. Nat Commun. 9:2165. PubMed
  4. Gadkari M, et al. 2018. Sci Data. 5:180275. PubMed
  5. Anderson NR, et al. 2019. Cell Adh Migr. 13:163. PubMed
  6. Sim J, et al. 2019. MAbs. 11:1036. PubMed
  7. Banks DA, et al. 2019. J Immunol. 202:2348. PubMed
  8. Shokri MR, et al. 2019. Sci Rep. 9:10007. PubMed
  9. Fielding CA et al. 2017. eLife. 6 pii: e22206. PubMed
  10. Chen S, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 316:L280. PubMed
  11. Bassaganyas L, et al. 2019. J Cell Biol. 218:3861. PubMed
  12. Wu Y, et al. 2019. Sci Rep. 9:20301. PubMed
  13. Lee JK, et al. 2020. Sci Rep. 2.567361111. PubMed
  14. Foight GW, et al. 2019. Nat Biotechnol. 37:1209. PubMed
  15. Mendoza-Cabrera MI, et al. 2019. Innate Immun. :1753425919864658. PubMed
  16. Shiba T, et al. 2019. J Allergy Clin Immunol. 144:1438. PubMed
  17. Liang Y, et al. 2019. J Allergy Clin Immunol. 144:1025. PubMed
  18. Grandclaudon M, et al. 2019. Cell. 179:432. PubMed
  19. Dent JR, et al. 2019. J Appl Physiol (1985). 127:1117. PubMed
  20. Robinet P, et al. 2014. J Immunol. 192:5332. PubMed
  21. Ameres S, et al. 2014. J Immunol. 192:5894. PubMed
  22. Zoldan K, et al. 2014. Dev Comp Immunol. 47:254. PubMed
  23. Braun F, et al. 2015. PLoS One. 10:117994. PubMed
  24. Huang X, et al. 2015. PLoS One. 10: 0133152. PubMed
  25. Lokmic Z, et al. 2015. J Vis Exp. 99: 52691. PubMed
  26. Schatzlmaier P, et al. 2015. Sci Signal. 8: rs11. PubMed
  27. Chew G, et al. 2016. PLoS Pathog. 12: 1005349. PubMed
  28. Wright A, et al. 2016. J Immunol. 196: 4877 - 4884. PubMed
  29. Sugita S, et al. 2016. Stem Cell Reports. 7:619-634. PubMed
  30. Samuel RM, et al. 2020. Cell Stem Cell. 27:876. PubMed
  31. Tuttle KD, et al. 2020. Cell Rep. 33:108407. PubMed
  32. Peng H, et al. 2020. Ann Transl Med. 0.927777778. PubMed
  33. Foltz L, et al. 2020. Mol Biol Cell. 31:2269. PubMed
  34. Junghans V, et al. 2020. J Cell Sci. 133:00:00. PubMed
  35. Meng KP, et al. 2020. J Exp Med. 217:00:00. PubMed
  36. Stephens M, et al. 2021. Sci Rep. 1.385416667. PubMed
  37. Iwabuchi R, et al. 2021. Frontiers in Immunology. 12:643040. PubMed
  38. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  39. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
RRID
AB_2847829 (BioLegend Cat. No. 400112)

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 02/22/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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