Alexa Fluor® 647 anti-Podoplanin (Lymphatic Endothelial Marker) Antibody

Pricing & Availability
Clone
D2-40 (See other available formats)
Other Names
Podoplanin, T1-alpha, hT1alpha-1, hT1alpha-2, PA2.26 antigen, glycoprotein 36, lung type-I cell membrane-associated glycoprotein (T1A-2)
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
D2-40_A647_Podoplanin_Antibody_050619.png
Human paraffin-embedded breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human podoplanin (clone D2-40) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
  • D2-40_A647_Podoplanin_Antibody_050619.png
    Human paraffin-embedded breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with Sodium Citrate H.I.E.R. 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human podoplanin (clone D2-40) Alexa Fluor® 647 (red) at 4°C overnight. Nuclei were counterstained with DAPI (green). The image was captured with a 10X objective.
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
916609 25 µg £97
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916610 100 µg £178
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Description

Podoplanin is expressed by a variety of normal cells, which include breast and prostate myopepithelial cells, follicular dendritic cells, basal keratinocytes of the skin and cervix (focal), type I pneumocytes, ependymal cells, and fetal cerebral germinal matrix cells. Podoplanin is a marker of lymphatic differentiation because it is expressed by normal human lymphatic endothelium, but not by vascular endothelium. It is diagnostically a useful marker for the evaluation of a variety of neoplasms, including Kaposi sarcoma mesothelioma, testicular germ cell tumors, and cutanneous sebaceous neoplasms.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
M2A protein derived from human germ cell tumors.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA Detection5 and immunocytochemistry4.

Application References

(PubMed link indicates BioLegend citation)
  1. Hamanaka T, et al. 2011. Invest. Ophthalmol. Vis. Sci. 52:8849. (IHC-P)
  2. Choi WW, et al. 2005. Mod. Pathol. 18:143. (IHC-P)
  3. Chu AY, et al. 2005. Mod. Pathol. 18:105. (IHC-P) PubMed
  4. Yoon C, et al. 2008. Blood 112:1129. (ICC) PubMed
  5. Schacht V, et al. 2005. American Journal of Pathology 166:913. (ELISA Detection, WB) PubMed
  6. Marks A, et al. 1999. British Journal of Cancer 80:569.

Antigen Details

Structure
35 kD.
Distribution

Membrane and plasma membrane.

Function
May be involved in cell migration and/or actin cytoskeleton organization. When expressed in keratinocytes, induces changes in cell morphology with transfected cells showing an elongated shape, numerous membrane protusions, major reorganization of the actin cytoskeleton, increased motility and decreased cell adhesion. Required for normal lung cell proliferation and alveolus formation at birth. Induces platelet aggregation. Does not have any effect on folic acid or amino acid transport and does not function as a water channel or as a regulator of aquaporin-type water channels.
Interaction
Cytoskeletal signaling.
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

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Go To Top Version: 1    Revision Date: 05/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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