Anti-Prox1 Antibody (Previously Covance catalog# PRB-238C)

Pricing & Availability
Clone
Poly19252 (See other available formats)
Other Names
Prospero Homeobox Protein 1, Prox-1, Prospero-Related Homeobox 1, Homeobox Prospero-Like Protein Prox1
Previously
Covance Catalog# PRB-238C
Isotype
Rabbit Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly19252_Anti-Prox1_Serum_IF_1_030315.jpg
Prox1 (Poly19252) detection on mouse brain. Photo courtesy of C. Zhou & S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_IF_1_030315.jpg
    Prox1 (Poly19252) detection on mouse brain. Photo courtesy of C. Zhou & S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_IF_2_030315.jpg
    Prox1 (Poly19252) detection on mouse brain. Photo courtesy of S. Pleasure, UCSF.
  • Poly19252_Anti-Prox1_Serum_WB_3_030315.jpg
    Total lysates (15 µg protein) from HepG2 (Positive control) and HL-60 (Low expression negative control) were resolved by electrophoresis (4-20% Tris-glycine gel), transferred to nitrocellulose, and probed with 1:500 purified Prox1 antibody, clone Poly19052. Proteins were visualized using chemiluminescence detection by incubation with HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401, 1:3000 dilution). Direct-Blot™ HRP anti-β-actin was used as a loading control (Cat. No. 643807, 1:8000 dilution).
Cat # Size Price Quantity Avail. Save
925202 25 µl £50
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925201 200 µl £202
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Description

Prox1 is a prospero related homeobox gene. Its expression pattern suggests it has a role in a variety of embryonic tissues. Prox1-deficient mice are devoid of lymphatic vasculature, and in these animals endothelial cells fail to acquire the lymphatic phenotype; instead, they remain as blood vascular endothelium.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
The Prox1 antiserum was generated against the C-terminal 15 amino acids of mouse Prox1.
Formulation
Stored in 50% Glycerol and 0.03% Thimerosal.
Preparation
Serum
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
Store between 2°C and 8°C.
Application

IHC-F - Quality tested
WB - Validated
ICC - Reported in literature

Recommended Usage

Each lot of this antibody is quality control tested by immunohistochemical staining on frozen tissue sections. For immunohistochemistry, a dilution of 1:500 is suggested. For Western blotting, the suggested use of this reagent is 1:500 dilution. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).

*Expected MW: 83 kD

WB Positive Control: HEK293 Lysate

IHC Positive Control: Mouse Brain


This product may contain other non-IgG subtypes.

Application References

(PubMed link indicates BioLegend citation)
  1. Pierreux CE, et al. 2004. J. Biol. Chem. 279:51298.
  2. Bagri A, et al. 2003. Cell. 113:285.
  3. Bagri A, et al. 2002. Development. 129:4249.
Product Citations
  1. Castillo S, et al. 2016. Sci Transl Med. 8: 332ra43. PubMed
Publication Library
RRID
AB_2749865 (BioLegend Cat. No. 925201)

Antigen Details

Cell Type
Neural Stem Cells
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Signal Transduction, Stem Cells
Gene ID
5629 View all products for this Gene ID
UniProt
View information about Prox1 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/26/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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