Alexa Fluor® 594 anti-human Podoplanin Antibody

Pricing & Availability
Clone
LpMab-21 (See other available formats)
Other Names
T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
LpMab-21_A594_Podoplanin_Antibody_1_071919
Human paraffin-embedded breast cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% TritonX-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human Podoplanin (clone LpMab-21) Alexa Fluor® 594 (red) and anti-Vimentin (clone O91D3) Alexa Fluor® 647 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
  • LpMab-21_A594_Podoplanin_Antibody_1_071919
    Human paraffin-embedded breast cancer tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% TritonX-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human Podoplanin (clone LpMab-21) Alexa Fluor® 594 (red) and anti-Vimentin (clone O91D3) Alexa Fluor® 647 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
  • LpMab-21_A594_Podoplanin_Antibody_2_071919
    Human paraffin-embedded normal breast tissue slices were prepared with a standard protocol of deparaffinization and rehydration. Antigen retrieval was done with citrate buffer pH 6.0 1X at 95°C for 40 minutes. Tissue was washed with PBS/0.05% Tween 20 twice for five minutes, permealized with 0.5% TritonX-100 for 10 minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 10 µg/mL of anti-human Podoplanin (clone LpMab-21) Alexa Fluor® 594 (red) and anti-Vimentin (clone O91D3) Alexa Fluor® 647 (green) at 4°C overnight. Nuclei were counterstained with DAPI (blue). The image was scanned with a 10X objective and stitched with Metamorph® software.
See Alexa Fluor® 594 spectral data
Cat # Size Price Quantity Avail. Save
395005 25 µg £91
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395006 100 µg £171
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Description

Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human podoplanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 594 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 594.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 594 has an excitation maximum of 590 nm, and a maximum emission of 617 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Application References

(PubMed link indicates BioLegend citation)
  1. Kaneko MK, et al. 2017. Cancer Med. 6(2):382-396 (IHC-P, FC)

Antigen Details

Structure
40-43 kD type-I transmembrane sialomucin-type glycoprotein
Distribution

Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells

Function
Lymphatic vessel formation, tumor metastasis, platelet aggregation
Ligand/Receptor
CLEC2
Biology Area
Cancer Biomarkers, Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers
Antigen References
  1. Raica M, et al. 2008. Anticancer Res. 28:2997.
  2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
  3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
  4. Kato Y, et al. 2003. J. Biol. Chem. 278:51599.
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 07/19/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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