Alexa Fluor® 647 anti-human Podoplanin Antibody

Pricing & Availability
Clone
LpMab-21 (See other available formats)
Other Names
T1a, T1a2, gp36, gp38, gp40, Aggrus, PDPN, D2-40
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
LpMab-21_A647_Podoplanin_Antibody_122118.png
Human LN319 cell line was stained with anti-human podoplanin (clone LpMab-21) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
  • LpMab-21_A647_Podoplanin_Antibody_122118.png
    Human LN319 cell line was stained with anti-human podoplanin (clone LpMab-21) Alexa Fluor® 647 (filled histogram) or mouse IgG2a, κ isotype control (clone MOPC-173) Alexa Fluor® 647 (open histogram).
See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
395003 25 tests £91
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395004 100 tests £205
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Description

Podoplanin is a 40-43 kD type-I transmembrane sialomucin-type glycoprotein, also known as T1a, gp36, gp38, gp40, and Aggrus. Originally detected on the surface of podocytes, futher characterization showed podoplanin has a broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and a variety of tumor cells. It has been reported that podoplanin is the ligand of CLEC2 and is involved in lymphatic vessel formation, platelet aggregation, and tumor metastasis. Podoplanin may serve as a useful marker for tumor diagnosis and prognosis.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant human podoplanin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions. The solution is free of unconjugated Alexa Fluor® 647.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633 nm / 635 nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Red Laser (633 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kaneko MK, et al. 2017. Cancer Med. 6(2):382-396 (IHC-P, FC)
RRID
AB_2783309 (BioLegend Cat. No. 395003)
AB_2783310 (BioLegend Cat. No. 395004)

Antigen Details

Structure
40-43 kD type-I transmembrane sialomucin-type glycoprotein
Distribution

Broad tissue distribution, including mesothelial cells, epithelial cells, follicular dendritic cells, and many tumor cells

Function
Lymphatic vessel formation, tumor metastasis, platelet aggregation
Ligand Receptor
CLEC2
Biology Area
Cell Biology, Neuroinflammation, Neuroscience, Neuroscience Cell Markers, Cancer Biomarkers
Antigen References
  1. Raica M, et al. 2008. Anticancer Res. 28:2997.
  2. Xie Q, et al. 2008. Int. J. Clin. Exp. Pathol. 1:276.
  3. Ogasawara S, et al. 2008. Hybridoma. 27:259.
  4. Kato Y, et al. 2003. J. Biol. Chem. 278:51599.
Gene ID
10630 View all products for this Gene ID
UniProt
View information about Podoplanin on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 12/21/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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