Recombinant Mouse TNF-α (carrier-free)

Pricing & Availability
Regulatory Status
Other Names
Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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Product Citations
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
  • TNFa_Mouse_Recombinant_Protein_BA_072519_updated.png
    Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D.
Cat # Size Price Quantity Check Availability Save
575202 10 µg 118€
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575204 25 µg 193€
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575206 100 µg 516€
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575208 500 µg 1384€
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TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Product Details

Mouse TNF-α, amino acids Leu80-Leu235 (Accession # NM_013693), was expressed in E. coli.
Molecular Mass
The 156 amino acid recombinant protein has a predicted molecular mass of 17,257 Da. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Leu.
Purity is >98%, as determined by Coomassie stained SDS-PAGE.
0.22 µm filtered protein solution is in 10mM NaH2PO4, 150mM NaCl, pH 7.2.
Endotoxin Level
Endotoxin level is <0.1 EU/µg (<0.01ng/µg) protein as determined by the LAL method.
10 and 25 µg sizes are bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 - 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 - 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Cytotoxic effect on L929 mouse fibroblast cells induced by mouse TNF-α in the presence of actinomycin D. The ED50 for this effect is 0.5 – 3 pg/mL.


Recommended Usage

Use when high specific biological activity is required.

Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are verified in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at

Application References

(PubMed link indicates BioLegend citation)
  1. Alcaide P, et al. 2012. J. Immunol. 188:1421. PubMed
  2. Azcutia V, et al. 2012. J. Immunol. 189:2553. PubMed
  3. Theiss AL, et al. 2009. Mol. Biol. Cell. 20:4412. PubMed
Product Citations
  1. Kuonen F, et al. 2021. Cell Rep. 37:109774. PubMed
  2. Kakiuchi N, et al. 2020. Nature. 260:577. PubMed
  3. Mc Fie M, et al. 2020. J Cell Sci. . PubMed
  4. Burke TP, et al. 2020. Nat Microbiol. 0.686111111. PubMed
  5. Inazumi T, et al. 2020. Cell Rep. 108265:33. PubMed
  6. Zeng J, et al. 2019. Cell Rep. 27:549. PubMed
  7. Hore ZL, et al. 2021. Wellcome Open Res. 6:68. PubMed
  8. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  9. Foley KP, et al. 2021. J Biol Chem. 296:100440. PubMed
  10. Tapryal N, et al. 2021. J Biol Chem. :100723. PubMed
  11. Iizasa E, et al. 2021. Nat Commun. 12:2299. PubMed
  12. Hanley KL, et al. 2022. Mol Cancer Res. 20:337. PubMed
  13. Wahida A, et al. 2021. Sci Immunol. 6:eabf7235. PubMed
  14. Mabe NW, et al. 2020. Cell Rep. 33:108341. PubMed
  15. Liu Z, et al. 2021. Immunity. 54(2):247-258.e7. PubMed
  16. Jung H, et al. 2019. Autophagy. 15:1990. PubMed
  17. Paraschivescu C, et al. 2022. Front Behav Neurosci. 16:845458. PubMed
  18. Li C, et al. 2021. Cell Metabolism. 33(8):1610-1623.e5. PubMed
  19. Thiele S, et al. 2020. Cell Rep. 31:107549. PubMed
  20. Mc Fie M, et al. 2020. J Cell Sci. 133:00:00. PubMed
  21. Bradley W, et al. 2016. Infect Immun . 84: 998-1015. PubMed
  22. Cartwright ANR, et al. 2021. Cancer Immunol Res. 9:470. PubMed
  23. Lien TS, et al. 2021. Front Immunol. 12:618577. PubMed
  24. Riebeling T, et al. 2020. Cell Death Differ. . PubMed
  25. Grosserichter-Wagener C, et al. 2020. MAbs. 12:1814661. PubMed
  26. Walens A, et al. 2019. Elife. 8:e43653. PubMed
  27. VanDussen KL, et al. 2019. Stem Cell Res. 37:101430. PubMed
  28. Hao L, et al. 2021. Br J Pharmacol. 178:4726. PubMed
  29. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  30. Kim SJ, et al. 2020. EMBO Rep. 21:e49332. PubMed
  31. Aggarwal N, et al. 2021. Cell Rep. 37:110170. PubMed
  32. Liberti DC, et al. 2021. Cell Rep. 35:109092. PubMed

Antigen Details

TNF superfamily; dimer/trimer
Paracrine/endocrine mediator of inflammatory and immune functions; selectively cytotoxic for transformed cells; endothelial cell alterations; chemoattractant
Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Targets
Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia
TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists
Gene ID
21926 View all products for this Gene ID
View information about TNF-alpha on

Related FAQs

Why choose BioLegend recombinant proteins?

     • Each lot of product is quality-tested for bioactivity as indicated on the data sheet.
     • Greater than 95% Purity or higher, tested on every lot of product.
     • 100% Satisfaction Guarantee for quality performance, stability, and consistency.
     • Ready-to-use liquid format saves time and reduces challenges associated with reconstitution.
     • Bulk and customization available. Contact us.
     • Learn more about our Recombinant Proteins.

How does the activity of your recombinant proteins compare to competitors?

We quality control each and every lot of recombinant protein. Not only do we check its bioactivity, but we also compare it against other commercially available recombinant proteins. We make sure each recombinant protein’s activity is at least as good as or better than the competition’s. In order to provide you with the best possible product, we ensure that our testing process is rigorous and thorough. If you’re curious and eager to make the switch to BioLegend recombinants, contact your sales representative today!

What is the specific activity or ED50 of my recombinant protein?

The specific activity range of the protein is indicated on the product datasheets. Because the exact activity values on a per unit basis can largely fluctuate depending on a number of factors, including the nature of the assay, cell density, age of cells/passage number, culture media used, and end user technique, the specific activity is best defined as a range and we guarantee the specific activity of all our lots will be within the range indicated on the datasheet. Please note this only applies to recombinants labeled for use in bioassays. ELISA standard recombinant proteins are not recommended for bioassay usage as they are not tested for these applications.

Have your recombinants been tested for stability?

Our testing shows that the recombinant proteins are able to withstand room temperature for a week without losing activity. In addition the recombinant proteins were also found to withstand four cycles of freeze and thaw without losing activity.

Does specific activity of a recombinant protein vary between lots?

Specific activity will vary for each lot and for the type of experiment that is done to validate it, but all passed lots will have activity within the established ED50 range for the product and we guarantee that our products will have lot-to-lot consistency. Please conduct an experiment-specific validation to find the optimal ED50 for your system.

How do you convert activity as an ED50 in ng/ml to a specific activity in Units/mg?

Use formula Specific activity (Units/mg) = 10^6/ ED50 (ng/mL)

Go To Top Version: 4    Revision Date: 07.25.2019

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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