Purified anti-mouse CD28 (Maxpar® Ready) Antibody

Product Details

Verified Reactivity

Mouse

Antibody Type

Monoclonal

Host Species

Syrian Hamster

Immunogen

C57BL/6 mouse T-cell lymphoma EL-4

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA.

Preparation

The antibody was purified by affinity chromatography.

Concentration

1.0 mg/ml

Storage & Handling

The CD28 antibody solution should be stored undiluted between 2°C and 8°C.

Application

FC - Quality tested
CyTOF® - Verified

Recommended Usage

This product is suitable for use with the Maxpar® Metal Labeling Kits. For metal labeling using Maxpar® Ready antibodies, proceed directly to the step to Partially Reduce the Antibody by adding 100 µl of Maxpar® Ready antibody to 100 µl of 4 mM TCEP-R in a 50 kDa filter and continue with the protocol. Always refer to the latest version of Maxpar® User Guide when conjugating Maxpar® Ready antibodies.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation¹, in vitro costimulation of T and NK cells¹, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity^3,4, in vitro induction of thymocyte differentiation^2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).

Additional Product Notes

Maxpar® is a registered trademark of Standard BioTools Inc.

Application References

(PubMed link indicates BioLegend citation)

1. Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
2. Cibotti R, et al. 1997. Immunity 6:245. (Costim)
3. Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
4. Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
5. Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
6. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
7. Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
8. Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
9. Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
10. Perchonock CE, et al. 2007. J. Immunol. 179:1768.
11. Barbi J, et al. 2007. Blood 110:2215.
12. Milpied P, et al. 2011. Blood 118:2993. PubMed
13. Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
14. Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
15. Li CR, et al. 2014. J Immunol. 192:1425. PubMed
16. Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed

Product Citations

1. Wei SC et al. 2017. Cell. 170(6):1120-1133 . PubMed

RRID

AB_2563736 (BioLegend Cat. No. 102119)

Disclaimer

This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.

Antigen Details

Structure

Ig superfamily, 44 kD

Distribution

Thymocytes, CD4⁺, CD8⁺ peripheral T cells, NK cells

Function

Costimulates T and NK cells

Ligand/Receptor

CD80 (B7-1), CD86 (B7-2)

Cell Type

NK cells, T cells, Thymocytes, Tregs

Biology Area

Costimulatory Molecules, Immunology

Molecular Family

CD Molecules

Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.

Gene ID

12487 View all products for this Gene ID

UniProt

View information about CD28 on UniProt.org

Documentation

• Technical data sheet
• Certificate of Analysis
• Safety Data Sheet

Reviews

Related Protocols

• Cell Surface Flow Cytometry Staining Protocol

Related Pages & Pathways

Related FAQs

Can I obtain CyTOF data related to your Maxpar® Ready antibody clones?

We do not test our antibodies by mass cytometry or on a CyTOF machine in-house. The data displayed on our website is provided by Fluidigm^®. Please contact Fluidigm^® directly for additional data and further details.

http://techsupport.fluidigm.com/

Can I use Maxpar® Ready format clones for flow cytometry staining?

We have not tested the Maxpar^® Ready antibodies formulated in solution containing EDTA for flow cytometry staining. While it is likely that this will work in majority of the situations, it is best to use the non-EDTA formulated version of the same clone for flow cytometry testing. The presence of EDTA in some situations might negatively affect staining.

I am having difficulty observing a signal after conjugating a metal tag to your Maxpar® antibody. Please help troubleshoot.

We only supply the antibody and not test that in house. Please contact Fluidigm^® directly for troubleshooting advice: http://techsupport.fluidigm.com/

Is there a difference between buffer formulations related to Maxpar® Ready and purified format antibodies?

The Maxpar^® Ready format antibody clones are formulated in Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and EDTA. The regular purified format clones are formulated in solution that does not contain any EDTA. Both formulations are however without any extra carrier proteins.

Other Formats

Concentration & Expiration Lookup

Certificate of Analysis