- 37.51 (See other available formats)
- Regulatory Status
- Other Names
- Tp44, T44
- Syrian Hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
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CD28 is a 44 kD glycoprotein, also known as Tp44 or T44. It is a member of the Ig superfamily, expressed on thymocytes, most peripheral T cells, and NK cells. In association with CD80 (B7-1) and CD86 (B7-2), CD28 acts as the second signal for T and NK cell activation and proliferation. The 37.51 antibody has been reported to augment in vitro T cell proliferation and cytokine production, and promote CTL development.Product Details
- Verified Reactivity
- Antibody Type
- Host Species
- Syrian Hamster
- C57BL/6 mouse T-cell lymphoma EL-4
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- The antibody was purified by affinity chromatography and conjugated with KIRAVIA Blue 520™ under optimal conditions.
- 0.2 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
* KIRAVIA Blue 520™ has an excitation maximum of 495 nm, and a maximum emission of 520 nm.
KIRAVIA Blue™ 520 is a trademark of Sony. This product is subject to proprietary rights of Sony and is made and sold under license from Sony Corporation. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Sony and the Sony logo are registered trademarks of Sony Corporation.
- Excitation Laser
Blue Laser (488 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro costimulation of T and NK cells1, in vitro blocking of allogeneic mixed leukocyte response and inhibition of MHC-unrestricted CTL cytotoxicity3,4, in vitro induction of thymocyte differentiation2,5-9,11, and immunohistochemical staining of acetone-fixed frozen sections. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 102116).
(PubMed link indicates BioLegend citation)
- Gross JA, et al. 1992. J. Immunol. 149:380. (IP, Costim)
- Cibotti R, et al. 1997. Immunity 6:245. (Costim)
- Masten BJ, et al. 1997. Am. J. Respir. Cell Mol. Biol. 16:335. (Block)
- Nishio M, et al. 1996. J. Immunol. 157:4347. (Block)
- Zhang N and He Y-W, 2005. J. Exp. Med. 202:395. (Costim)
- Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Costim)
- Perchonock CE, et al. 2006. Mol Cell Biol. 26(16):6005. (Costim)
- Wang W, et al. 2007. J. Immunol. 178:4885. (Costim)
- Pua HH, et al. 2007. J. Exp. Med. 204:25. (Costim)
- Perchonock CE, et al. 2007. J. Immunol. 179:1768.
- Barbi J, et al. 2007. Blood 110:2215.
- Milpied P, et al. 2011. Blood 118:2993. PubMed
- Cunningham NR, et al. 2011. Int Immunol. 23:693. PubMed
- Crispin JC, et al. 2012. J. Immunol. 188:3567. PubMed
- Li CR, et al. 2014. J Immunol. 192:1425. PubMed
- Blankenhaus B, et al. 2014. PLoS Pathog. 10:1003913. PubMed
AB_2890669 (BioLegend Cat. No. 102135)
AB_2890669 (BioLegend Cat. No. 102136)
- Ig superfamily, 44 kD
Thymocytes, CD4+, CD8+ peripheral T cells, NK cells
- Costimulates T and NK cells
- CD80 (B7-1), CD86 (B7-2)
- Cell Type
- NK cells, T cells, Thymocytes, Tregs
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Lenschow DJ, et al. 1996. Annu. Rev. Immunol. 14:233.
3. Gross JA, et al. 1992. J. Immunol. 149:380.
- Gene ID
- 12487 View all products for this Gene ID
- View information about CD28 on UniProt.org
Related Pages & Pathways
Other FormatsView All CD28 Reagents Request Custom Conjugation
|APC anti-mouse CD28||37.51||FC|
|Biotin anti-mouse CD28||37.51||FC|
|PE anti-mouse CD28||37.51||FC|
|PE/Cyanine5 anti-mouse CD28||37.51||FC|
|Purified anti-mouse CD28||37.51||FC,IP,IHC-F,Costim,Block|
|PerCP/Cyanine5.5 anti-mouse CD28||37.51||FC|
|Ultra-LEAF™ Purified anti-mouse CD28||37.51||FC,IP,IHC-F,Costim,Block|
|Purified anti-mouse CD28 (Maxpar® Ready)||37.51||FC,CyTOF®|
|PE/Cyanine7 anti-mouse CD28||37.51||FC|
|PE/Dazzle™ 594 anti-mouse CD28||37.51||FC|
|Brilliant Violet 421™ anti-mouse CD28||37.51||FC|
|TotalSeq™-C0204 anti-mouse CD28||37.51||PG|
|KIRAVIA Blue 520™ anti-mouse CD28||37.51||FC|
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
APC anti-mouse CD28
Biotin anti-mouse CD28
PE anti-mouse CD28
PE/Cyanine5 anti-mouse CD28
Purified anti-mouse CD28
PerCP/Cyanine5.5 anti-mouse CD28
Ultra-LEAF™ Purified anti-mouse CD28
Purified anti-mouse CD28 (Maxpar® Ready)
PE/Cyanine7 anti-mouse CD28
PE/Dazzle™ 594 anti-mouse CD28
Brilliant Violet 421™ anti-mouse CD28
TotalSeq™-C0204 anti-mouse CD28
KIRAVIA Blue 520™ anti-mouse CD28