PE anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Regulatory Status
RUO
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
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Product Citations
publications
bc96
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
  • bc96
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
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302605 25 tests 32€
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302606 100 tests 76€
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Chimpanzee, Cynomolgus, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Ajith A, et al. 2021. Front Immunol. 12:687715. PubMed
  2. Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed
  3. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  4. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  5. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  6. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  7. Netsrithong R, et al. 2020. Stem Cell Research. 39:101487.. PubMed
  8. Freeman ZT, et al. 2020. J Clin Invest. 130:1405. PubMed
  9. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
  10. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  11. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  12. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  13. Wei H, et al. 2021. Malar J. 20:89. PubMed
  14. Xun X, et al. 2021. Am J Transl Res. 13:4360. PubMed
  15. Vinogradova EV, et al. 2021. STAR Protoc. 2:100458. PubMed
  16. Saadati S, et al. 2020. Avicenna J Med Biotechnol. 0.591666667. PubMed
  17. Klemm F, et al. 2020. Cell. 181(7):1643-1660.e17. PubMed
  18. Delacher M, et al. 2021. Immunity. 54(4):702-720.e17. PubMed
  19. Pothlichet J, et al. 2022. Front Immunol. 13:824746. PubMed
  20. Bellini N, et al. 2022. iScience. 25:105234. PubMed
  21. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  22. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  23. Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed
  24. Ramien C, et al. 2020. Cell Reports. 29(4):810-815. PubMed
  25. Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed
  26. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  27. Kobayashi Y, et al. 2020. Int J Oncol. 999:56. PubMed
  28. James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed
  29. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  30. Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed
  31. Meléndez AV, et al. 2022. Cell Mol Life Sci. 79:513. PubMed
  32. Lee JM, et al. 2022. Lupus Sci Med. 9:. PubMed
  33. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  34. Shevyrev D, et al. 2021. Exp Ther Med. 209:21. PubMed
  35. Robertson N, et al. 2020. J Biol Chem. . PubMed
  36. Wu D, et al. 2020. Biomark Res. 8:3. PubMed
  37. Chen Y, et al. 2021. Transl Lung Cancer Res. 10:2193. PubMed
  38. Erdem A, et al. 2022. Nat Commun. 13:1105. PubMed
  39. You M, et al. 2021. Nat Cell Biol. 23:620. PubMed
  40. Carnevale J, et al. 2022. Nature. 609:174. PubMed
  41. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  42. Scarneo SA, et al. 2022. Sci Rep. 12:18091. PubMed
  43. Shen X, et al. 2021. Front Immunol. 12:710750. PubMed
  44. Mengrelis K, et al. 2021. Pediatr Transplant. 25:e13930. PubMed
  45. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  46. Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed
  47. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  48. Batikan O, et al. 2022. J Coll Physicians Surg Pak. 32:1004. PubMed
  49. Houtsma R, et al. 2021. STAR Protoc. 2:100864. PubMed
  50. Cohen CA, et al. 2021. Nat Commun. 12:4678. PubMed
  51. Keefe RC, et al. 2021. Sci Rep. 11:14933. PubMed
  52. Tian M, et al. 2021. Elife. 10:. PubMed
  53. Chruewkamlow N, et al. 2021. Stem Cell Res Ther. 12:520. PubMed
  54. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  55. Imura Y, et al. 2020. JCI Insight. :5. PubMed
  56. Vinogradova EV, et al. 2020. Cell. 182(4):1009-1026.e29. PubMed
  57. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  58. Hirschberger S, et al. 2021. EMBO Mol Med. 13:e14323. PubMed
  59. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  60. Zhang B, et al. 2020. Cell Chemical Biology. 28(1):60-69.e7. PubMed
  61. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
  62. Csomós K, et al. 2022. Nat Immunol. 23:1256. PubMed
  63. Maas RR, et al. 2021. Nat Protoc. 16:4692. PubMed
  64. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  65. Ajith A, et al. 2019. FASEB J. 33:5220. PubMed
  66. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  67. Loo Yau H, et al. 2021. STAR Protocols. 2(2):100549. PubMed
  68. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  69. Robertson N, et al. 2020. J Biol Chem. 295:18436. PubMed
  70. Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed
  71. Fierle JK, et al. 2021. Cell Reports Medicine. 2(8):100362. PubMed
  72. Hui Z, et al. 2022. Cell Death Dis. 13:607. PubMed
  73. Högelin KA, et al. 2021. iScience. 24:103078. PubMed
  74. Choe JH, et al. 2021. Sci Transl Med. 13:. PubMed
  75. Wang AS, et al. 2020. Molecular Cell. 79(2):221-233.e5.. PubMed
  76. Kim N, et al. 2020. Nat Commun. 2.045138889. PubMed
  77. Welch JL, et al. 2020. FEBS Lett. 594:695. PubMed
RRID
AB_314275 (BioLegend Cat. No. 302605)
AB_314276 (BioLegend Cat. No. 302606)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
B cells, Macrophages, Monocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 3    Revision Date: 12.15.2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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