PE anti-human CD25 Antibody

Pricing & Availability
Clone
BC96 (See other available formats)
Workshop
V T-072
Other Names
Low affinity IL-2R, IL-2R α chain, Tac, p55
Isotype
Mouse IgG1, κ
Ave. Rating
5 reviews
Product Citations
publications
bc96
PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
  • bc96
    PHA-stimulated (3 day) human peripheral blood lymphocytes were stained with CD25 (clone BC96) PE (solid line) or mouse IgG1, κ PE isotype control (dotted line).
See PE spectral data
Cat # Size Price Quantity Avail. Save
302605 25 tests 28€
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302606 100 tests 68€
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Description

CD25 is a 55 kD type I transmembrane glycoprotein also known as the low affinity IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Baboon, Chimpanzee, Pigtailed Macaque, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The CD25 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications include: immunocytochemistry3.

Application References

(PubMed link indicates BioLegend citation)
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Kmieciak M, et al. 2009. J. Transl. Med. 7:89. (FC) PubMed
  3. Ernst CW, et al. 2007. Clin. Exp. Immunol. 148:271. (ICC) PubMed
Product Citations
  1. Zhang W, et al. 2012. J Biol Chem. 287:34157. PubMed
  2. Chen Y, et al. 2012. PLoS One. 7:e47190. PubMed
  3. Ferraro A, et al. 2014. Proc Natl Acad Sci U S A. 111:1111. PubMed
  4. Reichwald K, et al. 2014. PLoS One. 9:105627. PubMed
  5. Han L, et al. 2014. J Biol Chem. 289:25546. PubMed
  6. Fujigaki J, et al. 2015. PLoS One. 10: 0132521. PubMed
  7. Luo X, et al. 2015. J Biol Chem. 290: 28675 - 28682. PubMed
  8. James E, et al. 2016. PLoS Pathog. 12: 1005375. PubMed
  9. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  10. Lima J, et al. 2016. Reprod Sci. 10.1177/1933719116653680. PubMed
  11. Marco Barros R, et al. 2016. Cell. 167: 203-218. PubMed
  12. Zhou Y, et al. 2016. J Leukoc Biol. 100: 1201 - 1211. PubMed
  13. Cerny D, et al. 2016. J Allergy Clin Immunol. 138:1709-1712. PubMed
  14. Riner D, et al. 2016. PLoS Negl Trop Dis. 10:e0005180. PubMed
  15. Wang B, et al. 2017. Stem Cell Res Ther.. 10.1186/s13287-017-0744-6. PubMed
  16. Goletz C, et al. 2018. Front Immunol. 9:1614. PubMed
  17. Wang L, et al. 2018. Oncol Lett. 15:8635. PubMed
  18. Meng S, et al. 2018. Mol Med Rep. 18:4247. PubMed
  19. Savage TM, et al. 2018. JCI Insight. 3:. PubMed
  20. Lopatina T, et al. 2018. Sci Rep. 8:17458. PubMed
  21. Li X, et al. 2019. Mol Med Rep. 19:4195. PubMed
  22. Tang M, et al. 2019. Biomed Res Int. 2019:1050285. PubMed
  23. de Boer B et al. 2018. Cancer cell. 34(4):674-689 . PubMed
  24. Montel‐Hagen A et al. 2019. Cell stem cell. 24(3):376-389 . PubMed
  25. Miragaia RJ, et al. 2019. Immunity. 50:493. PubMed
  26. Iwahori K, et al. 2019. Sci Rep. 2.205555556. PubMed
  27. Yang M, et al. 2019. Cell Physiol Biochem. 52:1178. PubMed
  28. Soodgupta D, et al. 2019. Cell Rep. 29:829. PubMed
  29. Miao L, et al. 2020. Theranostics. 0.7625. PubMed
RRID
AB_314275 (BioLegend Cat. No. 302605)
AB_314276 (BioLegend Cat. No. 302606)

Antigen Details

Structure
Type I transmembrane glycoprotein, 55 kD
Distribution

Activated T cells and B cells, monocytes/macrophages, Treg

Function
Associates with IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
Ligand/Receptor
IL-2
Cell Type
T cells, B cells, Monocytes, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681.

Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06.05.2013

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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