ELISA MAX™ Deluxe Set Mouse TNF-α

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
Ave. Rating
Submit a Review
Product Citations
publications
mouse tnf-a_122109
  • mouse tnf-a_122109
Cat # Size Price Quantity Check Availability Save
430904 5 plates 244€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430915 10 Plates 469€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
430916 20 Plates 751€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price!

BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse TNF-α ELISA MAX™ Capture Antibody (200X)
  • Mouse TNF-α ELISA MAX™ Detection Antibody (200X)
  • Mouse TNF-α Standard
  • Avidin-HRP (1000X)
  • Substrate Solution A
  • Substrate Solution B
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA

 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application References

(PubMed link indicates BioLegend citation)
  1. Patole PS, et al. 2005. J. Am. Soc. Nephrol. 16:3273.
  2. ChavantF, et al. 2010. J. Pharmacol Exp. Ther. 332:505. PubMed
  3. Lewis CJ, et al. 2010. J. Immunol. 184:3789. PubMed
  4. Qui J, et al. Appl Environ Microbiol. PubMed
  5. Yang HJ, et al. 2011. J Cell Sci. 124:2552. PubMed.
  6. White LE, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  7. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  8. Zachman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  9. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  10. Zahman AL, et al. 2014. Biomaterials. 35:9635. PubMed
  11. Justice JN, et al. 2015. J appl Physiol. 118:163. PubMed
Product Citations
  1. Luo W, et al. 2018. Bioorg Med Chem. 26:1547. PubMed
  2. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  3. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  4. Chou WC, et al. 2021. Nature. 591:300. PubMed
  5. Ishikawa S, et al. 2021. Biol Pharm Bull. 44:550. PubMed
  6. Cerrato G, et al. 2021. Cell Death Differ. 28:1733. PubMed
  7. Kim MW, et al. 2020. Front Bioeng Biotechnol. 8:133. PubMed
  8. Li DY, et al. 2018. Sci Rep. 8:17979. PubMed
  9. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  10. Chen M, et al. 2022. J Nanobiotechnology. 20:273. PubMed
  11. Radwanska M, et al. 2021. Vaccines (Basel). 9:. PubMed
  12. Du Q, et al. 2019. Front Microbiol. 10:2050. PubMed
  13. Yang Y, et al. 2020. J Int Med Res. 48:300060520902605. PubMed
  14. Nagata K, et al. 2020. Int Immunol. 32:143. PubMed
  15. Ma X, et al. 2022. Cell Mol Immunol. . PubMed
  16. Kalafatakis I, et al. 2021. J Neurosci Res. 99:1474. PubMed
  17. Yang W, et al. 2020. Nat Commun. 3.553472222. PubMed
  18. Zachman A, et al. 2014. Biomaterials. 35:9635. PubMed
  19. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  20. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  21. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  22. Clausen B, et al. 2016. Sci Rep. 6:29291. PubMed
  23. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  24. Lv ZY, et al. 2022. J Inflamm Res. 15:2819. PubMed
  25. Li H, et al. 2021. Nat Commun. 12:2773. PubMed
  26. Ye H, et al. 2016. Exp Mol Pathol. 100: 307-311. PubMed
  27. Kohn M, et al. 2021. Front Immunol. 580594:12. PubMed
  28. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  29. Yang H, et al. 2011. J Cell Sci. 124:2552. PubMed
  30. Park HB, et al. 2020. Oncoimmunology. 9:1772663. PubMed
  31. Schaafsma W, et al. 2017. Neurobiology of Disease. 10.1016/j.nbd.2017.07.017. PubMed
  32. Ahangar P, et al. 2020. NPJ Regen Med. 24:5. PubMed
  33. Brunetti J, et al. 2016. J Biol Chem. 291(49):25742-25748. PubMed
  34. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  35. Lehmann M, et al. 2016. J Leukoc Biol. 99: 1057 - 1064. PubMed
  36. Park GT, et al. 2021. Mar Drugs. 19:. PubMed
  37. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  38. Qiu J, et al. 2010. Appl Envir Microbiol. 76:5846. PubMed
  39. Dai X, et al. 2017. FASEB J. 31:2881. PubMed
  40. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  41. Zhang X, et al. 2014. PLoS One. 9:90856. PubMed
  42. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  43. Hsu CY, et al. 2022. Int J Mol Sci. 23:. PubMed
  44. Chen G, et al. 2021. Sci Adv. 7:eabg5686. PubMed
  45. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  46. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  47. Medrano–Jiménez E, et al. 2019. J Neuroinflammation. 16:143. PubMed
  48. Park GT, et al. 2019. Front Immunol. 10:2095. PubMed
  49. Iannucci J, et al. 2020. Cell Mol Neurobiol. . PubMed
  50. Yu Y, et al. 2021. Cell Rep. 37:110126. PubMed
  51. Wang J, et al. 2022. J Clin Invest. :. PubMed
  52. Lee N, et al. 2022. Front Immunol. 13:940258. PubMed
  53. Hsueh SC, et al. 2022. Biomedicines. 10:. PubMed
  54. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  55. Xiahou Z,et al. 2017. Nat Commun. . 10.1038/s41467-017-00930-9. PubMed
  56. Al Mamun A, et al. 2020. Proc Natl Acad Sci U S A. 117:1742. PubMed
  57. Ci T, et al. 2020. Sci Adv. 6:00. PubMed
  58. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  59. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  60. Fan Q, et al. 2021. Bioact Mater. 6:4014. PubMed
  61. Zhou XM, et al. 2018. Front Pharmacol. 8:904. PubMed
  62. Sun B, et al. 2018. Cell Mol Immunol. 1.168055556. PubMed
  63. Li WS, et al. 2021. Int J Mol Sci. 22:. PubMed
  64. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  65. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  66. Wang C, et al. 2020. Redox Biol. 37:101688. PubMed
  67. White L, et al. 2012. Am J Physiol Lung Cell Mol Physiol. 303:449. PubMed
  68. Wang Y, et al. 2021. Nat Commun. 12:4964. PubMed
  69. Justice J, et al. 2015. J Appl Physiol. 118:163. PubMed
  70. Chan K, et al. 2015. J Biol Chem. 290: 16979 - 16988. PubMed
  71. Stanfield BA, et al. 2021. PLoS One. 16:e0245169. PubMed
  72. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  73. Feng L, et al. 2022. Signal Transduct Target Ther. 7:21. PubMed
  74. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  75. Scanu A, et al. 2022. Int J Mol Sci. 23:. PubMed
  76. Deng LR, et al. 2022. Chin Med. 17:132. PubMed
  77. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  78. Nagata K, et al. 2021. Front Immunol. 12:730706. PubMed
  79. Gu H, et al. 2021. Elife. 10:. PubMed
  80. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  81. Lanfermann C, et al. 2021. Vaccines (Basel). 9:. PubMed
  82. de León-Guerrero SD, et al. 2022. Dis Model Mech. . PubMed
  83. Su S, et al. 2022. Signal Transduct Target Ther. 7:137. PubMed
  84. Kohn M, et al. 2021. Front Immunol. 626627:12. PubMed
  85. Goldberg R, et al. 2014. Diabetes. 63:4302. PubMed
  86. Li X, et al. 2022. Sci Rep. 12:17058. PubMed
  87. Agrahari G, et al. 2021. Front Immunol. 12:628117. PubMed
  88. Cobb C 2010. J Immunol. 184:3789. PubMed
  89. Chavant F, et al. 2010. J Pharmacol Exp Ther. 332:505. PubMed
  90. Reed MD, et al. 2020. Nature. 577:249. PubMed
  91. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
  92. Fante T, et al. 2016. PLoS One. 11: 0160184. PubMed
  93. Ratna A, et al. 2021. Hepatol Commun. 5:1165. PubMed
Sensitivity
4 pg/mL
Standard Range
7.8-500 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 4    Revision Date: 12.14.2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere

Login / Register
Remember me
Forgot your password? Reset password?
Create an Account