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Regulatory Status
Other Names
TNF-α Pre-coated ELISA Kit
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Product Citations
human tnf-a_010510
  • human tnf-a_010510
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430207 1 Pre-coated Plate 328€
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Human TNF-α (Tumor Necrosis Factor α), also termed Cachectin, Cytotoxic Factor (CF), CTX, Hemorrhagic Factor, Macrophage-Derived Cytotoxic Factor, Macrophage Cytotoxic Factor (MCF), and MCT, is a potent multifunctional cytokine which can exert regulatory, inflammatory and cytotoxic effects on a wide range of normal lymphoid and non-lymphoid cells and tumor cells. TNF-α is secreted by macro­phages, monocytes, neutrophils, T-cells (primarily CD4+ T cells), NK-cells, and many transformed cell lines. It exists as a soluble 17.5 kD protein and a 26 kD membrane-associated protein. The soluble form protein derives from the membrane form by proteolytic processing. The membrane form protein, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorrhagic necrosis of tumors in vivo, increased fibro­blast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. It has been shown that TNF-α plays a critical role in many physiological and patho­logical conditions, including neurodegenerative diseases and cancer.

BioLegend’s LEGEND MAX™ Human TNF-α ELISA Kit is a sandwich Enzyme-Linked Immunosorbent Assay (ELISA), in which, a Human TNF-α specific monoclonal antibody is pre-coated on a 96-well strip-well plate.

BioLegend’s LEGEND MAX™ Human TNF-α ELISA Kit is specifically designed for the accurate quantitation of Human TNF-α from cell culture supernatant, serum, plasma or other bodily fluids. It is ready-to-use, accurate, and sensitive.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human TNF-α Pre-coated 96-well Strip Microplate
  • Human TNF-α Dectection Antibody
  • Human TNF-α Standard
  • Avidin-HRP B
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution D
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity


Product Citations
  1. Chen J, et al. 2020. Mol Med Rep. 1.144444444. PubMed
  2. Gholamnezhadjafari R, et al. 2017. Innate Immun. 23:440. PubMed
  3. Ramirez-Perez S, et al. 2020. Molecules. 25:00:00. PubMed
  4. Dudau M, et al. 2021. Front Pharmacol. 12:737571. PubMed
  5. Holtkamp C, et al. 2019. PLoS One. 14:e0217269. PubMed
3.5 pg/mL
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Gene ID
7124 View all products for this Gene ID
View information about TNF-alpha on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 3    Revision Date: 09.04.2015

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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