Biotin anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM8_Biotin_090707
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
  • BM8_Biotin_090707
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
123105 50 µg 40€
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123106 500 µg 120€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
Product Citations
  1. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  2. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  3. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  4. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  5. Georgilis A et al. 2018. Cancer cell. 34(1):85-102 . PubMed
  6. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  7. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  8. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  9. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  10. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  11. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  12. Yuan D et al. 2017. Cancer cell. 31(6):771-789 . PubMed
  13. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  14. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  15. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  16. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  17. Yauch L, et al. 2010. J Immunol. 185:5405. PubMed
  18. Matsumoto S, et al. 2010. J Immunol. 184:1543. PubMed
  19. Zoller E, et al. 2011. J Exp Med. 208:1203. PubMed
  20. Sasaki O, et al. 2013. J Immunol. 191:2879. PubMed
  21. Knab L, et al. 2014. Mol Cancer Res. 12:1440. PubMed
  22. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  23. Wu W, et al. 2016. PLoS One. 11: 0159512. PubMed
  24. Xu S, et al. 2016. PLoS One. 11: 0163829. PubMed
  25. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
RRID
AB_893499 (BioLegend Cat. No. 123105)
AB_893501 (BioLegend Cat. No. 123106)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 10.25.2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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