Biotin anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_Biotin_090707
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
  • BM8_Biotin_090707
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with biotinylated BM8, followed by Sav-PE
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123105 50 µg 48€
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123106 500 µg 143€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Gordan S, et al. 2020. Cell Reports. 29(10):3033-3046.e4.. PubMed
  2. Hutter K, et al. 2022. Front Immunol. 13:967914. PubMed
  3. Sasaki O, et al. 2013. J Immunol. 191:2879. PubMed
  4. Knab L, et al. 2014. Mol Cancer Res. 12:1440. PubMed
  5. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  6. Yuan D et al. 2017. Cancer cell. 31(6):771-789 . PubMed
  7. Boccasavia VL, et al. 2021. Cell Reports. 34(11):108861. PubMed
  8. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  9. Iturri L, et al. 2021. Immunity. . PubMed
  10. Joachim R, Suber F, and Kobzik L 2017. Sci Rep. . 10.1038/s41598-017-16743-1. PubMed
  11. Georgilis A et al. 2018. Cancer cell. 34(1):85-102 . PubMed
  12. Hazama D, et al. 2020. Cell Chemical Biology. 27(9):1181-1191.e7. PubMed
  13. Suah AN, et al. 2021. J Clin Invest. 131:. PubMed
  14. Xu S, et al. 2016. PLoS One. 11: 0163829. PubMed
  15. Wu W, et al. 2016. PLoS One. 11: 0159512. PubMed
  16. Thion MS et al. 2018. Cell. 172(3):500-516 . PubMed
  17. Yauch L, et al. 2010. J Immunol. 185:5405. PubMed
  18. Zoller E, et al. 2011. J Exp Med. 208:1203. PubMed
  19. Goggi JL, et al. 2020. Mol Imaging Biol. 22:1392. PubMed
  20. Onodera T, et al. 2019. J Immunol. 203:3282. PubMed
  21. Naing A et al. 2019. Cell reports. 26(5):1242-1257 . PubMed
  22. Linde N, et al. 2018. Nat Commun. 9:21. PubMed
  23. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  24. Ruhland MK, et al. 2020. Cancer Cell. 37(6):786-799.e5. PubMed
  25. Khiew SH, et al. 2020. J Clin Invest. 130:3453. PubMed
  26. Kim AD, et al. 2021. Sci Rep. 11:24194. PubMed
  27. Onodera T, et al. 2021. Immunity. 54:2385. PubMed
  28. Zeng Z, et al. 2022. Oncogene. :. PubMed
  29. Akk A, et al. 2019. Mol Immunol. 114:629. PubMed
  30. Cianciaruso C, et al. 2020. Cell Reports. 27(10):3062-3080.e11.. PubMed
  31. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  32. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  33. Adachi Y, et al. 2019. Nat Commun. 10:3883. PubMed
  34. Miyauchi K, et al. 2016. Nat Immunol. 17:1447-1458. PubMed
  35. Bellomo A, et al. 2020. Immunity. 53(1):127-142.e7. PubMed
  36. Grayczyk JP et al. 2017. Cell host & microbe. 22(5):678-687 . PubMed
  37. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  38. Matsumoto S, et al. 2010. J Immunol. 184:1543. PubMed
  39. Werner A, et al. 2021. iScience. 24:103076. PubMed
  40. Calvente CJ, et al. 2019. J Clin Invest. 130:4091. PubMed
  41. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  42. Lutz J, et al. 2015. Nat Commun. 6: 8575. PubMed
  43. Crowe J, et al. 2020. PLoS Pathog. 16:e1008391. PubMed
  44. Moriyasu T, et al. 2018. PLoS Negl Trop Dis. 12:e0006197. PubMed
  45. Liao YC, et al. 2021. Front Immunol. 12:743030. PubMed
RRID
AB_893499 (BioLegend Cat. No. 123105)
AB_893501 (BioLegend Cat. No. 123106)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 10.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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