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Biotin anti-mouse NK-1.1 Antibody

Pricing & Availability
Clone
PK136 (See other available formats)
Regulatory Status
RUO
Other Names
NKR-P1C, NKR-P1B, Ly-55, CD161, CD161b, CD161c
Isotype
Mouse IgG2a, κ
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Product Citations
publications
PK136_Biotin_1_110210
C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_1_110210
    C57BL/6 mouse splenocytes were stained with CD49b (DX5) APC and biotinylated NK1.1 (clone PK136) (top) or mouse IgG2a isotype control, followed by Sav-PE (bottom).
  • PK136_Biotin_2_110210
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108703 50 µg 38€
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108704 500 µg 106€
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Description

NK-1.1 surface antigen, also known as CD161b/CD161c and Ly-55, is encoded by the NKR-P1B/NKR-P1C gene. It is expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Expression of NKR-P1C antigen has been correlated with lysis of tumor cells in vitro and rejection of bone marrow allografts in vivo. NK-1.1 has also been shown to play a role in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 and DX5 are commonly used as mouse NK cell markers.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
NK-1+ cells from mouse spleen and bone marrow
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1,2, complement-dependent cytotoxicity3, in vivo depletion4,5,9,10, mediation of in vitro redirected lysis6, blocking of NK cell function7, induction of proliferation8, immunohistochemical staining of frozen sections11, immunofluorescence microscopy11, and spatial biology (IBEX)16,17. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 108712).

Application References

(PubMed link indicates BioLegend citation)
  1. Carlyle JR, et al. 1999. J. Immunol. 162:5917. (IP)
  2. Sentman CL, et al. 1989. Hybridoma 8:605. (IP)
  3. Koo GC, et al. 1984. Hybridoma 3:301. (Cyt)
  4. Sentman CL, et al. 1989. J. Immunol. 142:1847. (Deplete)
  5. Koo GC, et al. 1986. J. Immunol. 137:3742. (Deplete)
  6. Karlhofer FM, et al. 1991. J. Immunol. 146:3662.
  7. Kung SK, et al. 1999. J. Immunol. 162:5876. (Block)
  8. Reichlin A, et al. 1998. Immunol. Cell Biol. 76:143.
  9. Drobyski W, et al. 1996. Blood 87:5355. (Deplete)
  10. Andoniou CE, et al. 2005. Nat. Immunol. 6:1011. (Deplete)
  11. Kanwar JR, et al. 2001. J. Natl. Cancer Inst. 93:1541. (IHC, IF)
  12. Kroemer A, et al. 2008. J. Immunol. 180:7818. PubMed
  13. Kim JY, et al. 2009. Exp Mol Med. 30:288. PubMed
  14. Bankoti J, et al. 2010. Toxicol. Sci. 115:422. (FC) PubMed
  15. Lee H, et al. 2014. Invest Ophthalmol Vis Sci. 55:2885. PubMed
  16. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  17. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Hu J, et al. 2019. Am J Transl Res. 3.043055556. PubMed
  2. Davis FM, et al. 2020. J Immunol. 2503:204. PubMed
  3. Chappaz S, et al. 2021. Cell Reports. 36(3):109430. PubMed
  4. Garza-Rodea A, et al. 2011. PLoS One. 6:e14493. PubMed
  5. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  6. Florentin J, et al. 2022. Front Immunol. 13:882484. PubMed
  7. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  8. Lin YH, et al. 2021. Front Immunol. 12:626418. PubMed
  9. Matson CA, et al. 2020. Proc Natl Acad Sci U S A. 117:14342. PubMed
  10. Krueger PD, et al. 2021. Immunity. 54(4):687-701.e4. PubMed
  11. Giambra V et al. 2018. Cell stem cell. 23(5):714-726 . PubMed
  12. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  13. Pokrovskii M, et al. 2020. EMBO J. 39:e104159. PubMed
  14. Xu X, et al. 2020. J Cell Biol. 219:00:00. PubMed
  15. Li Q et al. 2018. Immunity. 48(2):258-270 . PubMed
  16. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
  17. Wang T, et al. 2020. Blood Sci. 2:79. PubMed
  18. Dey A et al. 2019. The EMBO journal. 38(7) pii: e100293. PubMed
  19. Kimball A, et al. 2018. Arterioscler Thromb Vasc Biol. 38:1102. PubMed
  20. She L, et al. 2021. JCI Insight. 6:e143509. PubMed
  21. Paiva RS, et al. 2021. Eur J Immunol. 51:1968. PubMed
  22. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  23. Emgård J, et al. 2018. Immunity. 143:419. PubMed
  24. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  25. He J, et al. 2020. Cell Reports. 29(9):2718-2730.e6.. PubMed
  26. Wang L, et al. 2021. Cancer Immunol Res. 9:348. PubMed
  27. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  28. Kimball AS et al. 2019. Immunity. 51(2):258-271 . PubMed
  29. Xu L et al. 2017. Immunity. 47(3):538-551 . PubMed
  30. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  31. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  32. Shen Z, et al. 2021. iScience. 24:103014. PubMed
  33. Hutter K, et al. 2020. FEBS J. . PubMed
  34. Hulsmans M et al. 2017. Cell. 169(3):510-522 . PubMed
  35. Yao M, et al. 2020. Cell. 180(3):502-520. PubMed
  36. Dong S, et al. 2021. Nature. 591:117. PubMed
  37. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  38. Frodermann V, et al. 2019. Nat Med. 25:1761. PubMed
  39. Aschman T, et al. 2021. Int J Mol Sci. 22:. PubMed
  40. Zeng Z, et al. 2022. Oncogene. :. PubMed
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  42. Andersen L, et al. 2020. Cell Reports. 29(13):4447-4459.e6.. PubMed
  43. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  44. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  45. Uematsu T, et al. 2016. Sci Rep. 6:37815. PubMed
  46. Zhai X, et al. 2021. Sci Adv. 7:eabk0490. PubMed
  47. Gamrekelashvili J, et al. 2021. Bio Protoc. 11:e4007. PubMed
  48. Frohner IE, et al. 2020. Cell Rep. 30:3171. PubMed
  49. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  50. Vasamsetti SB, et al. 2018. Immunity. 49:93. PubMed
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  52. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  53. Nasarre P, et al. 2021. Cancers (Basel). 13: . PubMed
  54. Zhu Z, et al. 2013. Proc Natl Acad Sci U S A. 110:1282. PubMed
  55. Bajaña S, et al. 2022. iScience. 25:103732. PubMed
  56. Miyauchi K, et al. 2021. Nat Commun. 12:3789. PubMed
  57. Anderson DA, et al. 2022. J Exp Med. 219:. PubMed
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RRID
AB_313390 (BioLegend Cat. No. 108703)
AB_313391 (BioLegend Cat. No. 108704)

Antigen Details

Structure
NKR-P1 gene family
Distribution

NK and NK-T cells in the NK1.1 mouse strains (C57BL, FVB/N, NZB)

Function
NK cell activation, IFN-γ production, cytotoxic granule release
Cell Type
NK cells, NKT cells
Biology Area
Immunology, Innate Immunity
Antigen References

1. Lanier LL. 1997. Immunity 6:371.
2. Yokoyama WM, et al. 1993. Ann. Rev. Immunol. 11:613.
3. Koo GC, et al. 1986. J. Immunol. 137:3742.
4. Giorda R, et al. 1991. J. Immunol. 147:1701.

Gene ID
17059 View all products for this Gene ID
UniProt
View information about NK-1.1 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 12.08.2016

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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