PerCP anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
BM8_PerCP_122607
Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 PerCP
  • BM8_PerCP_122607
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages stained with BM8 PerCP
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123125 25 µg 104€
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123126 100 µg 272€
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Description

F4/80, also known as EMR1 or Ly71, is a 160 kD glycoprotein of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80 has been widely used as a murine macrophage marker. It is expressed on a majority of tissue macrophages, including macrophages in the lung, gut, peritoneal cavity, thymus, and red pulp of the spleen, Kupffer cells, Langerhans cells, microglia, and certain dendritic cells. It is not expressed on macrophages located in the T cell areas of the spleen, lymph node, or Peyer's patch. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for the induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PerCP under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

* PerCP has a maximum absorption of 482 nm and a maximum emission of 675 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. DeSouza-Vieira T, et al. 2020. Cell Rep. 33:108317. PubMed
  2. Kiepura A, et al. 2021. Int J Mol Sci. 22:. PubMed
  3. Lee S, et al. 2019. eLife. 0.333333333333333. PubMed
  4. Lyons J, et al. 2016. PLoS One. 11: 0149069. PubMed
  5. Ho T, et al. 2011. J Biol Chem. 286:35943. PubMed
  6. Antunes KH, et al. 2022. Front Immunol. 13:867022. PubMed
  7. Forni MF, et al. 2021. Front Oncol. 11:667715. PubMed
  8. Zhang Z, et al. 2021. Oncoimmunology. 10:1912472. PubMed
  9. Fan X, et al. 2015. Mol Cancer Ther. 14:681. PubMed
  10. Zhang S, et al. 2012. Infect Immun . 80:3952. PubMed
  11. Wang ECE, et al. 2019. Cell Stem Cell. 24:654. PubMed
  12. Burkhardt A, et al. 2014. J Immunol. 193:1468. PubMed
  13. Aguilera M, et al. 2021. Int J Mol Sci. 22:. PubMed
  14. Olivo Pimentel V, et al. 2021. J Immunother Cancer. 9:. PubMed
  15. Pizzurro GA, et al. 2021. Cancers (Basel). 13:. PubMed
  16. Soukup K, et al. 2017. Sci Rep. 10.1038/s41598-017-12208-7. PubMed
  17. Laurent J, et al. 2011. Cancer Res. 71:3781. PubMed
  18. Lai NY, et al. 2020. Cell. 180:33:00. PubMed
  19. Sethuraman SN, et al. 2020. Theranostics. 10:3397. PubMed
RRID
AB_893495 (BioLegend Cat. No. 123125)
AB_893483 (BioLegend Cat. No. 123126)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kupffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

How stable is PerCP/Cy5.5 tandem as compared to PerCP alone?

PerCP/Cy5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 10.25.2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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