APC/Cyanine7 anti-mouse F4/80 Antibody

Pricing & Availability
Clone
BM8 (See other available formats)
Regulatory Status
RUO
Other Names
EMR1, Ly71
Isotype
Rat IgG2a, κ
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Product Citations
publications
BM8_APCCyanine7_F4-80_Antibody_020119
Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
  • BM8_APCCyanine7_F4-80_Antibody_020119
    Thioglycolate-elicited Balb/c mouse peritoneal macrophages were stained with CD11b FITC and F4/80 (clone BM8) APC/Cyanine7 (left) or Rat IgG2a, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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123117 25 µg 111€
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123118 100 µg 260€
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Description

F4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen (but not on the macrophages located in T cell areas of the spleen, lymph node and Peyer's patch), Kuffer cells, Langerhans cells, and bone marrow stromal cells. F4/80 has also been shown on a subset of dendritic cells. The biological ligand of F4/80 has not been identified, but it has been reported that F4/80 is required for induction of CD8+ T cells-mediated peripheral tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Murine macrophages
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 1.0 µg per 106 cells in 100 µl. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen sections1,2 and formalin-fixed paraffin-embedded sections6,7, Western blotting, and spatial biology (IBEX)12,13.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Schaller E, et al. 2002. Mol. Cell. Biol. 22:8035. (IHC)
  2. Stevceva L, et al. 2001. BMC Clin Pathol. 1:3. (IHC)
  3. Kobayashi M, et al.2008. J. Leukoc. Biol. 83:1354. PubMed
  4. Poeckel D, et al. 2009. J. Biol Chem. 284:21077. PubMed
  5. Glass AM, et al. 2013. J. Immunol. 190:4830. PubMed
  6. Koehm S, et al. 2007. J. Allergy Clin. Immunol. 120:570. (IHC)
  7. Rankin AL, et al. 2010. J. Immunol. 184:1526. (IHC)
  8. Sasi SP, et al. 2014. J Biol Chem. 289:14178. PubMed
  9. Thakus VS, et al. 2014. Toxicol Lett. 230:322. PubMed
  10. Watson NB, et al. 2015. J Immunol. 194:2796. PubMed
  11. Hirakawa H, et al. 2015. PLoS One. 10:119360. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Bruand M, et al. 2021. Cell Reports. 36(3):109412. PubMed
  2. Grandjean CL, et al. 2021. Sci Adv. 7:. PubMed
  3. Ferrere G, et al. 2021. JCI Insight. 6:. PubMed
  4. Resende M, et al. 2017. J Immunol. 10.4049/jimmunol.1601701. PubMed
  5. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  6. Pilch Z, et al. 2018. Cancer Immunol Res. 6:930. PubMed
  7. Marques RM, et al. 2021. Cell Death Differ. 28:3140. PubMed
  8. Coleby R, et al. 2021. Clin Exp Rheumatol. :39. PubMed
  9. Zhang S, et al. 2021. Cell. 184(8):2151-2166.e16. PubMed
  10. Vicario N, et al. 2021. Cell Death Disease. 12(7):625. PubMed
  11. Culina S, et al. 2014. PLoS One. 9:88365. PubMed
  12. Kozijn AE, et al. 2019. Osteoarthritis Cartilage. 27:118. PubMed
  13. Zhao J, et al. 2019. Nat Commun. 10:899. PubMed
  14. Diao L, et al. 2022. iScience. 25:105511. PubMed
  15. Nawaz A, et al. 2022. Nat Commun. 13:7058. PubMed
  16. Andreas N, et al. 2021. Arthritis Res Ther. 23:222. PubMed
  17. Rybalko V, et al. 2015. PLoS One. 10: 0145550. PubMed
  18. Ohtsuki T, et al. 2016. J Virol. 90: 300 - 307. PubMed
  19. Ushio A, et al. 2017. Int J Mol Sci. 10.3390/ijms18061209. PubMed
  20. Wang X, et al. 2020. Signal Transduct Target Ther. 5:35. PubMed
  21. Bode K, et al. 2020. Cell Reports. 29(13):4435-4446.e9.. PubMed
  22. Nguyen SL, et al. 2021. Sci Rep. 11:4217. PubMed
  23. Li S, et al. 2021. Ann Transl Med. 9:1644. PubMed
  24. Dias AMM, et al. 2021. J Gastroenterol. 56:442. PubMed
  25. Chen C, et al. 2017. Cell Rep. 10.1016/j.celrep.2017.02.040. PubMed
  26. Herbst S, et al. 2013. Dis Model Mech. 6:643. PubMed
  27. Wang Y, et al. 2022. Front Immunol. 13:767630. PubMed
  28. Han YH, et al. 2021. Science. 373:. PubMed
  29. Forni MF, et al. 2021. Front Oncol. 11:667715. PubMed
  30. Ohta S, et al. 2013. J Immunol. 190:5927. PubMed
  31. Mason K, et al. 2013. J Immunol. 191:2457. PubMed
  32. Levine LS, et al. 2021. Immunity. 54(4):829-844.e5. PubMed
  33. Li J, et al. 2020. Cancer Discov. . PubMed
  34. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  35. Brykczynska U, et al. 2020. Cell Reports. 30(5):1627-1643.e7.. PubMed
  36. Li J, et al. 2020. Cancer Immunol Res. 0.529166667. PubMed
  37. Taguchi K, et al. 2015. J Urol. . PubMed
  38. Benechet AP, et al. 2019. Nature. 574:200. PubMed
  39. Domingos-Pereira S, et al. 2019. Cancer Immunol Res. 7:621. PubMed
  40. Adams NM et al. 2018. Immunity. 48(6):1172-1182 . PubMed
  41. Haase C, et al. 2022. Nat Methods. 19:1622. PubMed
  42. Peruzzotti-Jametti L, et al. 2021. PLoS Biol. 19:e3001166. PubMed
  43. Taguchi K, et al. 2014. J Am Soc Nephrol. 25:1680. PubMed
  44. Wiedemann GM, et al. 2020. Cell Rep. 33:108498. PubMed
  45. Li J, et al. 2018. Immunity. 49:178. PubMed
  46. Diaz–Salazar C, et al. 2020. Cell Rep. 32:108186. PubMed
  47. Gao S, et al. 2022. Cell Res. 32:38. PubMed
  48. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
  49. Xue N, et al. 2017. Sci Rep. 7:39011. PubMed
  50. Watanabe E, et al. 2020. Angiogenesis. 23(4):667-684. PubMed
  51. Haloul M, et al. 2019. Sci Rep. 9:14050. PubMed
  52. Guo M, et al. 2018. J Immunol. 200:1039. PubMed
  53. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  54. Yang M, et al. 2021. Cancer Immunol Immunother. 70:2467. PubMed
  55. Sharma A, et al. 2020. Cell. 183(2):377-394.e21. PubMed
  56. De Simone G, et al. 2021. Immunity. :. PubMed
  57. Pilch Z, et al. 2019. PLoS One. 14:e0210796. PubMed
  58. Sasaki T, et al. 2020. Cell Reports. 28(1):202-217.e7.. PubMed
  59. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  60. Bugler-Lamb AR, et al. 2021. Mol Metab. 49:101197. PubMed
  61. Grioni M, et al. 2021. Blood Adv. 5:2817. PubMed
  62. Bourque J, et al. 2021. Heliyon. 7:e08311. PubMed
  63. Shen M, et al. 2022. Nat Cancer. 3:60. PubMed
  64. Blriot C, et al. 2021. Immunity. :. PubMed
  65. Ni J, et al. 2020. Immunity. 52(6):1075-1087.e8. PubMed
  66. Zordan P, et al. 2013. Eur J Pharmacol. 715:296. PubMed
  67. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  68. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  69. Lebel MÈ, et al. 2020. Nat Commun. 3.051388889. PubMed
  70. Kolodkin-Gal D, et al. 2021. Gut. Online ahead of print. PubMed
  71. Taddio MF, et al. 2021. Mol Imaging Biol. 23:196. PubMed
  72. Nuro-Gyina PK, et al. 2021. J Immunol. 207:868. PubMed
  73. Bagadia P, et al. 2019. Nat Immunol. 20:1174. PubMed
  74. Takahashi F, et al. 2022. iScience. 25:104278. PubMed
  75. Moro T, et al. 2016. PLoS One. 11: 0146592. PubMed
  76. Dichtl S, et al. 2022. Life Sci Alliance. 5:. PubMed
  77. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  78. Natale CA, et al. 2018. Elife. 7. PubMed
  79. Zhu K, et al. 2022. EMBO Rep. 23:e54499. PubMed
  80. Xu JL, et al. 2022. Front Pharmacol. 13:911663. PubMed
  81. de Assis LVM, et al. 2022. Commun Biol. 5:461. PubMed
  82. Zhang W, et al. 2021. Front Immunol. 12:768435. PubMed
  83. Ni Y, et al. 2021. Theranostics. 11:9431. PubMed
  84. Casselli T, et al. 2021. PLoS Pathog. 17:e1009256. PubMed
  85. Klose R, et al. 2016. Nat Commun. 7:12528. PubMed
  86. Zhang D, et al. 2020. Signal Transduct Target Ther. 5:24. PubMed
  87. Peruzzotti-Jametti L, et al. 2018. Cell Stem Cell. 22:355. PubMed
  88. Leiria LO et al. 2019. Cell Metab. 30(4):768-783 . PubMed
  89. Yoshida H, et al. 2019. Cell. 176:897. PubMed
  90. Fedele C, et al. 2021. J Exp Med. 218: . PubMed
  91. Zhang Z, et al. 2020. Front Immunol. 11:583276. PubMed
  92. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  93. Deliyannis G, et al. 2021. JCI Insight. 6:. PubMed
  94. Wang M, et al. 2021. iScience. 24(7):102766. PubMed
  95. Kwok I, et al. 2020. Immunity. 53(2):303-318.e5. PubMed
  96. DeFalco T, et al. 2016. Cell Rep. 12: 1107-1119. PubMed
  97. Bertuzzi M, et al. 2014. PLoS Pathog. 10:1004413. PubMed
  98. Celià-Terrassa T, et al. 2017. Nat Cell Biol. 19:711. PubMed
  99. Geary CD et al. 2018. Cell reports. 24(8):1949-1957 . PubMed
  100. Herbst S, et al. 2015. EMBO Mol Med. 30:240. PubMed
  101. Cardoso M, et al. 2015. Infect Immun . 83: 3534-3544. PubMed
  102. Chen Z, et al. 2016. Innate Immunity. 22: 419 - 432. PubMed
  103. Kahn JH, et al. 2020. Endocrinology. 161(10):. PubMed
  104. Mellal K, et al. 2019. Sci Rep. 9:12903. PubMed
  105. Sameshima A, et al. 2015. J Endocrinol. 227: 25-36. PubMed
  106. Morrissey MA, et al. 2018. Elife. 7:e36688. PubMed
  107. Yáñez A et al. 2017. Immunity. 47(5):890-902 . PubMed
  108. Patterson DG, et al. 2021. J Immunol. 207:1798. PubMed
  109. Andreata F, et al. 2021. STAR Protoc. 2:100831. PubMed
  110. Ren X, et al. 2021. Cell Death Dis. 12:484. PubMed
RRID
AB_893489 (BioLegend Cat. No. 123117)
AB_893477 (BioLegend Cat. No. 123118)

Antigen Details

Structure
EGF-TM7 family member, 160 kD glycoprotein
Distribution

Majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and red pulp of spleen, Kuffer cells, Langerhans cells, bone marrow stromal cells, and a subset of dendritic cells

Function
Induction of immunological tolerance
Cell Type
Dendritic cells, Langerhans cells, Macrophages, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Antigen References

1. Austy JM and Gordon S. 1981. Eur. J. Immunol. 11:805.
2. Hume DA, et al. 1983. J. Exp. Med. 158:1522.
3. Ruedl C, et al. 1996. Eur. J. Immunol. 26:1801.
4. McKnight AJ, et al. 1996. J. Biol. Chem. 271:486.
5. Lin HH, et al. 2005. J. Exp. Med. 201:1615.

Gene ID
13733 View all products for this Gene ID
UniProt
View information about F4/80 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02.01.2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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