- M1/69 (See other available formats)
- Regulatory Status
- Other Names
- Heat Stable Antigen (HSA), Ly-52, Nectadrin
- Rat IgG2b, κ
- Ave. Rating
- Submit a Review
- Product Citations
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CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.Product Details
- Antibody Type
- Host Species
- C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
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- Excitation Laser
Red Laser (633 nm)
- Application Notes
Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).
(PubMed link indicates BioLegend citation)
- Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
- Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
- Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
- Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
- Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
- Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
- Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
- Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
- Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
- Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
- Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
- Product Citations
AB_493485 (BioLegend Cat. No. 101817)
AB_493484 (BioLegend Cat. No. 101818)
- GPI-linked sialoglycoprotein, 35-45 kD
B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells
- Regulates B cell proliferation and differentiation
- P-selectin, CD24
- Cell Type
- B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
- Biology Area
- Molecular Family
- CD Molecules
- Antigen References
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.
- Gene ID
- 12484 View all products for this Gene ID
- View information about CD24 on UniProt.org
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