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Purified anti-mouse CD24 Antibody

Pricing & Availability
Clone
M1/69 (See other available formats)
Regulatory Status
RUO
Other Names
Heat Stable Antigen (HSA), Ly-52, Nectadrin
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
M1slash69_Purified_080207
C57BL/6 mouse splenocytes stained with purified M1/69, followed by anti-rat IgG FITC
  • M1slash69_Purified_080207
    C57BL/6 mouse splenocytes stained with purified M1/69, followed by anti-rat IgG FITC
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101801 50 µg 44€
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101802 500 µg 184€
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Description

CD24 is a 35-45 kD protein also known as Heat Stable Antigen (HSA), Ly-52, or Nectadrin. It is a GPI-linked sialoglycoprotein expressed on lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, and dendritic cells. CD24 expression varies during T and B cell differentiation and is a useful marker for delineating various lymphocyte developmental stages. CD24 serves as an adhesion or costimulatory molecule involved in T and B lymphocyte activation and differentiation by homophilic binding or binding to CD62P.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
WB, IHC-F, IHC-P - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, in vitro induction of thymocyte maturation2, complement-mediated cytotoxicity3, and immunohistochemistry of acetone-fixed frozen sections4, formalin-fixed paraffin-embedded sections5 and zinc-fixed paraffin-embedded sections10. The Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 101845 and 101846).

Application References

(PubMed link indicates BioLegend citation)
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (WB)
  2. Crowley M, et al. 1989. Cell. Immunol. 118:108. (FA)
  3. Veillette A, et al. 1989. J. Exp. Med. 170:1671. (FA)
  4. Pandelakis A Flavell RA 1999 JEM 189:855 (FC, IHC)
  5. Liu JQ, et al. 2007 J. Immunol. 178:6227. (FC, IF)
  6. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed
  7. Rucci F, et al. 2010. Proc Natl Acad Sci USA. 107:3024. (FC) PubMed
  8. Teague TK, et al. 2010. Int Immunol. 22:387. (FC) PubMed
  9. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (FC) PubMed
  10. Chen CY, et al. 2008. Endocrinology. 10:1210. (FC, IHC) PubMed
  11. Qui Q, et al. 2010. J. Immunol. 184:1681. (FC) PubMed
Product Citations
  1. Hutton C, et al. 2021. Cancer Cell. 39:1227. PubMed
  2. Young G,et al. 2017. J Virol.. 10.1128/JVI.01507-17. PubMed
  3. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  4. Bajaña S, et al. 2021. Front Immunol. 11:577718. PubMed
  5. Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
  6. Krovi SH, et al. 2019. Proc Natl Acad Sci U S A. 116:22252. PubMed
  7. Dienz O, et al. 2020. J Immunol. 204:1521. PubMed
  8. Yang M, et al. 2020. Oncoimmunology. 9:1708064. PubMed
  9. Mann ER, et al. 2020. Sci Immunol. :5. PubMed
  10. Huang WJ, et al. 2021. Theranostics. 11:5539. PubMed
  11. Yan J, et al. 2020. Cancer Immunol Res. 8:356. PubMed
  12. Fiege JK, et al. 2019. PLoS Pathog. 15:e1008077. PubMed
  13. Ghanei Z, et al. 2020. Journal of Cellular Physiology. 235:7840. PubMed
  14. Xie D, et al. 2020. Eur J Immunol. 50:1729. PubMed
  15. Liu H, et al. 2020. J Immunol. 205:1207. PubMed
  16. Hoves S, et al. 2018. J Exp Med. 215:859. PubMed
  17. Tao H, et al. 2021. Front Immunol. 12:623280. PubMed
  18. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  19. Lopes N, et al. 2021. Nat Immunol. 22:179. PubMed
  20. Blriot C, et al. 2021. Immunity. :. PubMed
  21. Gubin MM, et al. 2018. Cell. 175:1014. PubMed
  22. Shissler SC, et al. 2020. Sci Rep. 10:8218. PubMed
  23. Evrard M et al. 2018. Immunity. 48(2):364-379 . PubMed
  24. Arkatkar T, et al. 2018. Kidney Int. 94:728. PubMed
  25. Schneppenheim J, et al. 2017. J Immunol. 199:172. PubMed
  26. Wu W, et al. 2017. Sci Rep. 7:44481. PubMed
RRID
AB_312834 (BioLegend Cat. No. 101801)
AB_312835 (BioLegend Cat. No. 101802)

Antigen Details

Structure
GPI-linked sialoglycoprotein, 35-45 kD
Distribution

B cells, granulyocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells

Function
Regulates B cell proliferation and differentiation
Ligand/Receptor
P-selectin, CD24
Cell Type
B cells, Dendritic cells, Epithelial cells, Erythrocytes, Granulocytes, Monocytes, Thymocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Aigner S, et al. 1997. Blood 89:3385.
3. Hough MR, et al. 1996. J. Immunol. 156:479.
4. Liu Y, et al. 1992. J. Exp. Med. 175:437.

Gene ID
12484 View all products for this Gene ID
UniProt
View information about CD24 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11.30.2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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