Biotin anti-human IL-6 Antibody

Pricing & Availability
MQ2-39C3 (See other available formats)
Regulatory Status
Other Names
Interleukin-6, B cell stimulating factor-2 (BSF-2), Cytotoxic T cell differentiation factor (CDF), Hepatocyte stimulating factor (HSF), Hybridoma/plasmacytoma growth factor (HPGF)
Rat IgG2a, κ
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Product Citations
  • HumanIL-6_061604
  • MQ2-39C3_Biotin_IL6_Antibody_IF_100412
    Human PBMCs, stimulated with 1 µg/mL of LPS for 8 h and treated with Brefeldin A during the last 4 h, were prepared by cytospin, fixed and permeabilized on a slide and then treated with endogenous biotin blocking kit (Vector labs). Slides were stained with anti-human IL-6 biotin (clone MQ2-39C3) and DyLight™ 649 streptavidin (green) and counterstained with DAPI (blue). Images were acquired with an Olympus FV10i confocal microscope. Images courtesy of Teresa Rodriguez, Darius Schneider, and Matthias von Herrath, LaJolla Institute for Allergy and Immunology.
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501201 50 µg 81€
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501202 500 µg 219€
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IL-6 is a potent lymphoid cell growth factor that stimulates the growth and survival of certain B cells and T cells. IL-6 plays a role in host defense, acute phase reactions, immune response, and hematopoiesis. IL-6 is expressed by T cells, B cells, monocytes, fibroblasts, hepatocytes, endothelial cells and keratinocytes. The MQ2-39C3 antibody can neutralize the bioactivity of natural or recombinant IL-6.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Antibody Type
Host Species
COS-7- expressed, recombinant human IL-6
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection, ELISPOT Detection - Quality tested
ICC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5 - 2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of IL-6 recombinant protein ranging from 1000 to 8 pg/mL are recommended for each ELISA plate. For use as an ELISPOT detection antibody, a concentration range of 1.0 - 4.0 µg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1-4: The biotinylated MQ2-39C3 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified MQ2-13A5 antibody (Cat. No. 5001102/501110) as the capture antibody.
Additional reported applications (for the relevant formats) include: neutralization1, Western blotting. The LEAF™ purified MQ2-13A5 antibody (Endotoxin Note: For testing human IL-6 in serum or plasma, BioLegend's ELISA Max™ Sets (Cat. No. 430501 to 430506) are specially developed and recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Abrams, J., et al. 1992. Immunol. Rev. 127:5.
  2. Abrams, J. 1995. Curr. Prot. Immunol.. John Wiley and Sons, New York. Unit 6.20.
  3. Gaines Das, R., et al. 1993. J. Immunol. Meth. 160:147.
  4. Lesmeister, M. J., et al. 2005. Reproductive Biology and Endocrinology 3:74.
Product Citations
  1. Fosdick MG, et al. 2022. Sci Rep. 12:13506. PubMed
  2. Carestia A, et al. 2019. Cell Rep. 28:896. PubMed
  3. Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
AB_2127615 (BioLegend Cat. No. 501201)
AB_2249232 (BioLegend Cat. No. 501202)

Antigen Details

Neuropoietic cytokine family; 26 kD (Mammalian)
Hematopoiesis; antigen-specific immune responses, inflammatory reactions; acute phase reaction
Cell Sources
T cells, B cells, macrophages, bone marrow stromal cells, fibroblasts, keratinocytes, mesangium, astrocytes, endothelial cells
Cell Targets
T cells, B cells, hepatocytes, cholinergic neurons
Heterodimer IL-6Rα (CD126)/IL-6Rβ (CD130; gp130)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Hirano, T. 1998. Intl. Rev. Immunol. 16:249.
3. Patterson, P. 1992. Curr. Opin. Neurobiol. 2:94.
4. Van Oers, M., et al. 1993. Ann. Hematology 66:219.

Upregulated by IL-1, PDGF, IFN-β, TNF-α, NGF, IL-17; downregulated by glucocorticoids IL-4, TGF-β
Gene ID
3569 View all products for this Gene ID
View information about IL-6 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-6 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-human IL-6 MQ2-39C3 ELISA Detection,ELISPOT Detection,ICC
Purified anti-human IL-6 MQ2-39C3 ELISA,WB
Go To Top Version: 2    Revision Date: 01.20.2021

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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