Purified anti-mouse/rat/human MCP-1 Antibody

Pricing & Availability
Clone
2H5 (See other available formats)
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Isotype
Armenian Hamster IgG
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Avail. Save
505901 50 µg 64€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
505902 500 µg 160€
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Rat, Human, Cross-Reactivity: Rhesus
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
CHO-expressed, recombinant mouse MCP-1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ELISA Capture - Quality tested
WB, IHC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5-10 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture1: The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,4,6: The LEAF™ purified antibody (Endotoxin in vivo and in vitro (Cat. No. 505906) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.

Application References
  1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
  2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
  3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
  4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
  5. Hancock, W. W., et al. 1997 Transplantation 64:696.
  6. Yu, R., et al. 2006 Obesity 14:1353.
  7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
  8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Product Citations
  1. Patsouris D, et al. 2009. J Biol Chem. 284:31223. PubMed
  2. Rutar M, et al. 2011. Invest Ophthalmol Vis Sci. 52:2379. PubMed
  3. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Publication Library
RRID
AB_315407 (BioLegend Cat. No. 505901)
AB_315408 (BioLegend Cat. No. 505902)

Antigen Details

Structure
CC chemokine; dimer; 13.8 kD (Mammalian)
Bioactivity
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
Receptors
CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.

Regulation
Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
UniProt
View information about CCL2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 09-02-2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

BioLegend Inc., 8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login / Register
Forgot your password? Reset password?
Create an Account