Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1 Antibody

Pricing & Availability
2H5 (See other available formats)
Regulatory Status
Other Names
Monocyte chemoattractant protein-1, Monocyte chemoattractant and activating factor (MCAF), JE, Small inducible cytokine A2 (SCYA2), HC-11, P6, Smooth muscle cell chemotactic factor (SMC-CF), CCL2
Hamster IgG
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Product Citations
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505911 100 µg 88€
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505912 1 mg 120€
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505913 5 mg 432€
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505914 25 mg 1127€
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505915 50 mg 1884€
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505916 100 mg 2707€
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Monocyte chemotactic protein-1 (MCP-1) also known as monocyte chemotactic and activating factor (MCAF) was identified based on its ability to chemoattract monocytes. Subsequently, MCP-1 has also been found to regulate adhesion molecule expression and cytokine production in monocytes. MCP-1 is identical to the product of the JE gene, a PDGF inducible gene. MCP-1 is a member of the beta (C-C) chemokine subfamily, known as CCL2. The 2H5 antibody reacts with mouse, rat, and human MCP-1. The 2H5 antibody can neutralize the bioactivity of natural or recombinant MCP-1.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse, Rat, Human
Reported Reactivity
Antibody Type
Host Species
CHO-expressed, recombinant mouse MCP-1
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.01 EU/µg of the protein (< 0.001 ng/µg of the protein) as determined by the LAL test.
The Ultra-LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
The antibody is bottled at the concentration indicated on the vial, typically between 2 mg/mL and 3 mg/mL. Older lots may have also been bottled at 1 mg/mL. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. This Ultra-LEAF™ solution contains no preservative; handle under aseptic conditions.

ELISA Capture - Quality tested
ELISPOT Capture, Neut, WB, IHC - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 5.0 - 10 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of MCP-1 recombinant protein ranging from 2000 to 15 pg/mL are recommended for each ELISA plate.

Application Notes

ELISA or ELISPOT Capture1: The Ultra-LEAF™ 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 4E2/MCP antibody (Cat. No. 506002) as the detecting antibody for the detection of mouse MCP-1. The purified 2H5 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated 5D3-F7 antibody (Cat. No. 502609) as the detecting antibody for the detection of human MCP-1.
Flow Cytometry2: The fluorochrome-labeled 2H5 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify MCP-1 -producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization1,4,6: The Ultra-LEAF™ purified antibody (Cat. Nos. 505911 & 505912) is recommended for neutralization.
Additional reported applications (for the relevant formats) include: Western blotting3, immunohistochemistry of paraformaldehyde-fixed, saponin-treated frozen tissue sections5, and acetone-fixed frozen tissue sections7.

Application References
  1. Luo, Y., et al. 1994. J. Immunol. 153:3708.
  2. Zhang, Y., et al. 2002. J. Immunol. 168:3088.
  3. Luo, Y., et al. 1999 J. Immunol. 163:3985.
  4. Morrison, B. E., et al. 2003 J. Clin. Invest. 112:1862.
  5. Hancock, W. W., et al. 1997 Transplantation 64:696.
  6. Yu, R., et al. 2006 Obesity 14:1353.
  7. Taal MW, et al. 2001. Am. J. Physiol. Renal. Physiol. 280:F343.
  8. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
Product Citations
  1. Feng H, et al. 2020. Cell Death Dis. 1.094444444. PubMed
AB_2820086 (BioLegend Cat. No. 505911)
AB_2820087 (BioLegend Cat. No. 505912)
AB_2820088 (BioLegend Cat. No. 505913)
AB_2820089 (BioLegend Cat. No. 505914)
AB_2820090 (BioLegend Cat. No. 505915)
AB_2820091 (BioLegend Cat. No. 505916)

Antigen Details

CC chemokine; dimer; 13.8 kD (Mammalian)
Chemoattractant; regulates adhesion molecule expression, cytokine production in monocytes; proliferation/activation of CC-chemokine activated killer cells
Cell Sources
Monocytes, vascular endothelial cells, smooth muscle, glomerular mesangial cells
Cell Targets
Monocytes, basophils, T cells, NK cells, immature dendritic cells
CCR2; CCR5; CCR10; D6; possibly L-CCR
Biology Area
Cell Biology, Neuroinflammation, Neuroscience
Molecular Family
Antigen References

1. Fitzgerald, K., et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Bischoff, S., et al. 1992. J. Exp. Med. 175:1271.
3. Charo, I., et al. 1994. P. Natl. Acad. Sci. USA 91:2752.
4. Jiang, Y., et al. 1992. J. Immunol. 148:2423.

Upregulated by IL-1, TNF-α, PDGF, TGF-β
Gene ID
20296 View all products for this Gene ID 24770 View all products for this Gene ID 6347 View all products for this Gene ID
View information about CCL2 on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Other Formats

View All CCL2 Reagents Request Custom Conjugation
Description Clone Applications
PE anti-mouse/rat/human MCP-1 2H5 ICFC
Purified anti-mouse/rat/human MCP-1 2H5 ELISA Capture,IHC,WB
Biotin anti-mouse/rat/human MCP-1 2H5 ELISA Detection,ELISPOT Detection
APC anti-mouse/rat/human MCP-1 2H5 ICFC
Ultra-LEAF™ Purified anti-mouse/rat/human MCP-1 2H5 ELISA Capture,ELISPOT Capture,IHC,Neut,WB
Go To Top Version: 2    Revision Date: 06-15-2022

For Research Use Only. Not for diagnostic or therapeutic use.


This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.


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