Purified anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
Ave. Rating
2 reviews
Product Citations
publications
1-M1slash70_Purified_042706
C57BL/6 mouse bone marrow cells were stained with purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
  • 1-M1slash70_Purified_042706
    C57BL/6 mouse bone marrow cells were stained with purified CD11b (clone M1/70) (filled histogram) or rat IgG2b, κ isotype control (open histogram), followed by anti-rat IgG FITC (gated on total cells).
  • 2-M170_Pure_CD11b_Antibody_IHC_102418
    Fresh, frozen mouse spleen was stained with purified CD11b clone M1/70 conjugated and detected with a Cy5 CODEX™ oligonucleotide duplex (red). Samples were counterstained with TCR FITC (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
101201 50 µg 20€
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101202 500 µg 76€
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F, CyTOF® - Validated
IP, ICC - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone M1/70 has been validated for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/μg, Azide-Free, 0.2 μm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Olver S, et al. 2005. Cancer Res. 66:571. PubMed
  2. Elgazar-Carmon V, et al. 2008. J Lipid Res. 49:1849. PubMed
  3. Guo Y, et al. 2008. Blood. 112:480. PubMed
  4. Kim D, et al. 2008. Blood. 112:1109. PubMed
  5. Napimoga M, et al. 2008. J Immunol. 180:609. PubMed
  6. Baumgartner C, et al. 2010. J Immunol. 184:573. PubMed
  7. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  8. Sun Y, et al. 2015. Proc Natl Acad Sci U S A. 112: 10401-10406. PubMed
  9. Peng J, et al. 2016. Nat Commun. 7:12029. PubMed
  10. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  11. Francis M, et al. 2017. Toxicol Sci. 155(1):182-195. PubMed
  12. Galbas T, et al. 2017. J Immunol. 198(2):852-861. PubMed
  13. Rissiek B, et al. 2017. Sci Rep. . 10.1038/s41598-017-16613-w. PubMed
  14. Madsen D, et al. 2017. Cell Rep.. 10.1016/j.celrep.2017.12.011. PubMed
  15. Bachran C, et al. 2017. Sci Rep.. 10.1038/s41598-017-17948-0. PubMed
  16. Nagatake T, et al. 2018. Int Immunol. 30:471. PubMed
  17. Peterson EJ, et al. 2019. Mol Syst Biol. 15:e8584. PubMed
  18. Torika N, et al. 2018. CNS Neurosci Ther. 24:231. PubMed
  19. Axelrod HD, et al. 2019. Mol Cancer Res. 17:356. PubMed
  20. Peng J, et al. 2019. Mol Brain. 12:71. PubMed
  21. An Y et al. 2018. Cell reports. 25(3):702-714 . PubMed
  22. Zhou N et al. 2018. Protein & cell. 10(2):87-103 . PubMed
  23. Wagner J et al. 2019. Cell. 177(5):1330-1345 . PubMed
  24. Zhou LJ et al. 2019. Cell Rep. 27(13):3844-3859 . PubMed
  25. Lechner AJ et al. 2017. Cell stem cell. 21(1):120-134 . PubMed
  26. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  27. Zareie P, et al. 2017. J Neuroinflammation. 0.630555556. PubMed
  28. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  29. Chevrier S, et al. 2018. Cell Syst. 0.675. PubMed
  30. Ortiz A, et al. 2019. Cancer Cell. 35:33:00. PubMed
  31. Arimura K, et al. 2017. Mucosal Immunol. 1.08125. PubMed
  32. He X, et al. 2017. Cancer Biol Ther. 0.815277778. PubMed
  33. Stacey MA, et al. 2017. J Clin Invest. 127:1463. PubMed
  34. Ajona D, et al. 2017. Cancer Discov. 0.773611111. PubMed
  35. Akiel M, et al. 2017. Cancer Res. 77:4014. PubMed
  36. Fino KK, et al. 2017. Sci Rep. 5.334722222. PubMed
  37. Kawabe T, et al. 2017. Sci Immunol. 2:eaam9315. PubMed
  38. Goel S, et al. 2017. Nature. 548:471. PubMed
  39. Yamasaki T, et al. 2018. J Virol. 92:e01458. PubMed
  40. Shirakawa K, et al. 2017. PLoS One. 12:e0186303. PubMed
  41. Nolin JD, et al. 2017. JCI Insight. 2:e94929. PubMed
  42. Regan–Komito D, et al. 2017. Front Immunol. 1.459027778. PubMed
  43. Chartrand K, et al. 2018. Front Immunol. 1.642361111. PubMed
  44. Cao Q, et al. 2018. Am J Physiol Renal Physiol. 314:F561. PubMed
  45. Burrello C, et al. 2018. Front Med (Lausanne). 5:21. PubMed
  46. Manni M, et al. 2018. Nat Immunol. 1.074305556. PubMed
  47. Wicki S, et al. 2018. Int J Mol Sci. 1.266666667. PubMed
  48. Pushalkar S, et al. 2018. Cancer Discov. 0.613194444. PubMed
  49. Lee JY, et al. 2018. Front Immunol. 0.678472222. PubMed
  50. Tong CF, et al. 2018. Am J Physiol Cell Physiol. 315:C409. PubMed
  51. Kikuchi K, et al. 2018. J Immunol. 201:635. PubMed
  52. Diao J, et al. 2018. J Immunol. 201:1306. PubMed
  53. Chung MI, et al. 2018. Am J Respir Cell Mol Biol. 59:706. PubMed
  54. Ito Y, et al. 2018. J Exp Med. 215:2617. PubMed
  55. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  56. McLeod RL, et al. 2018. Oncotarget. 9:34459. PubMed
  57. Haney MS, et al. 2018. Nat Genet. 50:1716. PubMed
  58. Wang Y, et al. 2019. Biomed Res Int. 2019:5756189. PubMed
  59. Tang B, et al. 2019. Oncolytic Virother. 8:03. PubMed
  60. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  61. Yang C, et al. 2019. MBio. 10:e00595-19. PubMed
  62. Hsieh JY, et al. 2019. APL Bioeng. 3:016103. PubMed
  63. Arnold IC, et al. 2019. PLoS Pathog. 15:e1007866. PubMed
  64. Andrews AR, et al. 2019. Cancer Cell Int. 0.955555556. PubMed
  65. Gartlan KH, et al. 2019. Blood Adv. 2.110416667. PubMed
  66. Larson–Casey JL, et al. 2019. J Clin Invest. 129:4962. PubMed
RRID
AB_312784 (BioLegend Cat. No. 101201)
AB_312785 (BioLegend Cat. No. 101202)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 12-13-2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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