Purified anti-mouse TER-119/Erythroid Cells Antibody

Pricing & Availability
Clone
TER-119 (See other available formats)
Regulatory Status
RUO
Other Names
Ly-76
Isotype
Rat IgG2b, κ
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Product Citations
publications
TER-119
C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • TER-119
    C57BL/6 bone marrow cells stained with TER-119 FITC and CD45 PE
  • Ter-119_pure_Ter119Erythroid_Antibody_2_102518
    Fresh, frozen mouse spleen was stained with purified TER-119/Erythroid Cells clone TER-119 conjugated and detected with a FITC CODEX™ oligonucleotide duplex (red). Samples were counterstained with B220 A647 (green). Data generated at Akoya Biosciences, Inc. using the CODEX™ technology.
Cat # Size Price Quantity Avail. Save
116201 50 µg 28€
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116202 500 µg 92€
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Description

The TER-119 antigen is a 52 kD glycophorin A-associated protein, also known as Ly-76. TER-119 is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not on erythroid colony-forming cells (BFU-E, blast-forming unit erythroid, or CFU-E, colony-forming unit erythroid).

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Day-14 fetal liver cells from a C57BL/6 mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
IHC-F - Verified
IHC-P, IP, WB - Reported in the literature, not verified in house

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

The TER-119 antibody is useful for distinguishing erythrocytes and cells in the erythroid lineage. Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, complement-mediated cytotoxicity3, and immunohistochemical staining of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections. Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 116253-116258).

Application References
  1. Kina T, et al. 2000. Br. J. Haematol. 109:280. (IP, WB)
  2. Vannucchi AM, et al. 2000. Blood 95:2559.
  3. Maraskovsky E, et al. 1996. J. Exp. Med. 184:1953. (CMCD)
  4. Grisendi S, et al. 2005. Nature 437:147. (FC)
  5. Bourdeau A, et al. 2007. Blood 109:4220.
  6. Chappaz S, et al. 2007. Blood 110:3862. (FC)
  7. Heuser M, et al. 2007. Blood 110:1639. (FC)
  8. Gough SM, et al. 2014. Cancer Discov. 4:564. PubMed
Product Citations
  1. Lin J, et al. 2017. Nat Commun. . 10.1038/s41467-017-01477-5. PubMed
  2. Winter C, et al. 2018. Cell Metab. 28:175. PubMed
  3. Patel MM, et al. 2018. J Am Heart Assoc. 7:e010690. PubMed
  4. Tahara N, et al. 2018. Dev Biol. 434:74. PubMed
  5. Goltsev Y et al. 2018. Cell. 174(4):968-981 . PubMed
  6. Dave K et al. 2017. eLife. 6 pii: e23382. PubMed
  7. Wang HC, et al. 2017. J Immunol. 198:3149. PubMed
  8. Shafer MER, et al. 2017. Stem Cell Reports. 1.040277778. PubMed
  9. Parigi SM, et al. 2018. Sci Rep. 0.440277778. PubMed
  10. Zhao L, et al. 2018. Nat Med. 24:1536. PubMed
  11. Yoshihara S, et al. 2019. Front Immunol. 0.545833333. PubMed
  12. Chen S, et al. 2018. Immunohorizons. 0.345138889. PubMed
  13. Malik N, et al. 2019. Sci Rep. 9:16917. PubMed
  14. Blume J, et al. 2019. Eur J Immunol. 49:121. PubMed
  15. Satoh–Takayama N, et al. 2020. Immunity. 52(4):635-649. PubMed
  16. Chen L, et al. 2019. Nat Commun. 3.553472222. PubMed
  17. Niaudet C, et al. 2019. Angiogenesis. 1.257638889. PubMed
  18. Chappaz S, et al. 2007. Blood. 110:3862. PubMed
  19. Gui J, et al. 2007. Int Immunol. 1.625694444. PubMed
  20. Heuser M, et al. 2007. Blood. 110:1639. PubMed
  21. Cheng P, et al. 2008. J Exp Med. 205:2235. PubMed
  22. Gough S, et al. 2014. Cancer Discovery. 4:564. PubMed
  23. Duong CN, et al. 2020. Arterioscler Thromb Vasc Biol. 40:378. PubMed
  24. Chamberlin T, et al. 2020. Cancer Research. 80(20):4465-4475. PubMed
  25. Heib T, et al. 2021. Cell Reports. 35(6):109102. PubMed
RRID
AB_313702 (BioLegend Cat. No. 116201)
AB_313703 (BioLegend Cat. No. 116202)

Antigen Details

Structure
Associated with glycophorin A, 52 kD
Distribution

Early proerythroblast to mature erythrocyte, but not BFU-E and CFU-E

Cell Type
Erythrocytes
Biology Area
Immunology
Antigen References

1. Kina T, et al. 2000. Br. J. Haematol. 109:280.
2. Ikuta K, et al. 1990. Cell 62:863.
3. Osawa M, et al. 1996. Weir's Handbook of Experimental Immunology. Vol. 2 pp. 66.1-66.5.

Gene ID
104231 View all products for this Gene ID
UniProt
View information about TER-119 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 04-23-2020

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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