Alexa Fluor® 647 anti-mouse/human CD11b Antibody

Pricing & Availability
Clone
M1/70 (See other available formats)
Regulatory Status
RUO
Other Names
αM integrin, Mac-1, Mo1, CR3, Ly-40, C3biR, ITGAM
Isotype
Rat IgG2b, κ
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Product Citations
publications
1_M1slash70_Alx647_052710
C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
  • 1_M1slash70_Alx647_052710
    C57BL/6 mouse bone marrow cells were stained with CD11b (clone M1/70) Alexa Fluor® 647 or rat IgG2b, κ Alexa Fluor® 647 isotype control (gated on total cells).
  • 2_M1-70_A647_CD11b_Antibody_2_092121.png
    Paraformaldehyde-fixed (4%), 500 µm-thick mouse spleen section was processed according to the Ce3D™ Tissue Clearing Kit protocol (Cat. No. 427701). The section was costained with anti-mouse CD169 (Siglec-1) Antibody (clone 3D6.112) Alexa Fluor® 594 at 5 µg/mL (yellow), and anti-mouse/human CD11b Antibody (clone M1/70) Alexa Fluor® 647 at 5 µg/mL (magenta). The section was then optically cleared and mounted in a sample chamber. The image was captured with a 10X objective using Zeiss 780 confocal microscope and processed by Imaris image analysis software.
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See Alexa Fluor® 647 spectral data
Cat # Size Price Quantity Avail. Save
101220 25 µg 68,00€
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101218 100 µg 156,00€
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Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Product Details
Technical Data Sheet (pdf)

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit (Lapine)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
C57BL/10 splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
3D IHC - Verified

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. For 3D immunohistochemistry on formalin-fixed tissues, a concentration of 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

* Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

View full statement regarding label licenses
Excitation Laser
Red Laser (633 nm)
Application Notes

Clone M1/70 has been verified for immunocytochemistry (ICC) and frozen immunohistochemistry (IHC-F).

Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1,4, in vitro blocking3,9,12, depletion2,8, immunofluorescence microscopy6,7,10, and immunohistochemistry of acetone-fixed frozen sections5,11-13. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) (Cat. No. 101248).

Application References
  1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. (IP)
  2. Ault K and Springer T. 1981. J. Immunol. 126:359. (Deplete)
  3. Springer TA, et al. 1982. Immunol. Rev. 68:171. (Block)
  4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. (IP)
  5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112. (IHC)
  6. Noel GJ, et al. 1990. J. Clin. Invest. 85:208. (IF)
  7. Allen LA and Aderem A. 1996. J. Exp. Med. 184:627 (IF)
  8. D'Amico A and Wu L. 2003. J. Exp. Med. 198:293. (Deplete)
  9. Brickson SJ, et al. 2003. Appl Physiol. 95:969. (Block)
  10. Clatworthy MR and Smith KG. 2004. J. Exp. Med. 199:717. (IF)
  11. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  12. Zhang Y, et al. 2002. J. Immunol. 168:3088. (IHC)
  13. Iwasaki A and Kelsall BL. 2001. J. Immunol. 166:4884 (IHC, FC)
  14. Tailleux L. 2003. J. Exp. Med. 197:121. (Block, FC)
  15. Olver S, et al. 2006. Cancer Research 66:571. (FC)
  16. Tan SL, et al. 2006. J. Immunol. 176:2872. (FC) PubMed
  17. Ponomarev ED, et al. 2006. J. Immunol. 176:1402. (FC)
  18. Dzhagalov I, et al. 2007. Blood 109:1620. (FC)
  19. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  20. Rasmussen JW, et al. 2006. Infect. Immun.74:6590. PubMed
  21. Napimoga MH, et al. 2008. J. Immunol. 180:609. PubMed
  22. Elqaraz-Carmon V, et al. 2008. J. Lipid. Res. 49:1894. PubMed
  23. Kim DD, et al. 2008. Blood 112:1109. PubMed
  24. Guo Y, et al. 2008. Blood 112:480. PubMed
  25. Norian LA, et al. 2009. Cancer Res. 69:3086. (FC) PubMed
  26. Baumgartner CK, et al. 2010. J. Immunol. 184:573. PubMed
  27. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  28. Whiteland J, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  29. Weber GF, et al. 2014. J Exp Med. 211:1243. PubMed
  30. Ashok A, et al. 2015. Toxicol Sci. 143:64. PubMed
  31. Price PJ, et al. 2015. J Immunol. 194:1164. PubMed
  32. Doni A, et al. 2015. J Exp Med. 212:905. PubMed
  33. Ferreira R, et al. 2016. J Infect Dis. 213: 669 - 673. PubMed
  34. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
  1. Sweet R, et al. 2017. J Immunol. 10.4049/jimmunol.1600861. PubMed
  2. Cunha LD et al. 2018. Cell. 175(2):429-441 . PubMed
  3. Li Z et al. 2018. Immunity. 49(4):640-653 . PubMed
  4. Pinho S et al. 2018. Developmental cell. 44(5):634-641 . PubMed
  5. Philip E Boulais et al. 2018. Immunity. 49(4):627-639 . PubMed
  6. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  7. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
  8. Balzano M et al. 2019. Cell reports. 26(12):3257-3271 . PubMed
  9. Pronin A, et al. 2019. Front Mol Neurosci. 12:36. PubMed
  10. Farsakoglu Y et al. 2019. Cell reports. 26(9):2307-2315 . PubMed
  11. Miller EB, et al. 2019. Proc Natl Acad Sci U S A. 116:16603. PubMed
  12. Karlen SJ, et al. 2018. J Neuroinflammation. 15:344. PubMed
  13. Abels ER et al. 2019. Cell Rep. 28(12):3105-3119 . PubMed
  14. Chen ST et al. 2019. Cell host & microbe. 25(4):602-616 . PubMed
  15. Pierce H, et al. 2017. Cell Stem Cell. 1.283333333. PubMed
  16. Nowak W, et al. 2020. EBioMedicine. 50:290-305.. PubMed
  17. Davidson S, et al. 2020. Cell Reports. 31(7):107628. PubMed
  18. Silva HM, et al. 2019. J Exp Med. 216:786. PubMed
  19. Oh B, et al. 2017. Plast Reconstr Surg Glob Open. 5:e1595. PubMed
  20. Maas SLN, et al. 2020. J Neuroinflammation. 17:120. PubMed
  21. Pham THM, et al. 2020. Cell Host & Microbe. 27(1):54-67.e5.. PubMed
  22. Rasmussen J, et al. 2006. Infect Immun. 74:6590. PubMed
  23. Jackson A, et al. 2014. J Leukoc Biol. 92:609. PubMed
  24. Sanders K, et al. 2015. Cancer Immunol Res. 3: 891-901. PubMed
  25. Richardson ET, et al. 2015. PLoS One. 10: 1371. PubMed
  26. Richardson E, et al. 2015. Infect Immun . 83: 2242-2254. PubMed
  27. Wongchana W, et al. 2015. J Immunol. 195: 5337 - 5346. PubMed
  28. Catarinella M, et al. 2016. EMBO Mol Med. 8: 155 - 170. PubMed
  29. Chen W, et al. 2016. Nat Commun. 7: 11302. PubMed
  30. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  31. Chen G, et al. 2017. Biosens Bioelectron. 10.1016/j.bios.2016.10.015. PubMed
  32. Halder LD, et al. 2020. Nat Commun. 2.077083333. PubMed
  33. Alberts A, et al. 2020. Front Immunol. 11:596103. PubMed
  34. Singh A, et al. 2020. Mol Oncol. 1.901388889. PubMed
  35. Kim SI, et al. 2020. Molecular Cancer Therapeutics. 20(1):173-182. PubMed
  36. Bade RM, et al. 2021. Molecular Oncology. . PubMed
RRID
AB_493546 (BioLegend Cat. No. 101220)
AB_389327 (BioLegend Cat. No. 101218)

Antigen Details

Structure
Integrin family, associates with integrin β2 (CD18), 170 kD
Distribution

Granulocytes, monocytes/macrophages, dendritic cells, NK cells, subsets of T and B cells

Function
Adhesion, chemotaxis
Ligand/Receptor
ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, fibrinogen
Cell Type
B cells, Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils, NK cells, T cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Springer TA. 1994. Cell 76:301.
3. Coxon A, et al. 1996. Immunity 5:653.

Gene ID
16409 View all products for this Gene ID 3684 View all products for this Gene ID
UniProt
View information about CD11b on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 09-21-2021

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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