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PerCP/Cyanine5.5 anti-human CD137L (4-1BB Ligand) Antibody

Pricing & Availability
Clone
5F4 (See other available formats)
Regulatory Status
RUO
Other Names
4-1BBL, CD137L, TNFSF9, 41BBL
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
5F4_PerCPCyanine55_CD137L_Antibody_012519
Human T lymphoblastic leukemia cell line, Hut-78, was stained with CD137L (4-1BB Ligand)(clone 5F4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
  • 5F4_PerCPCyanine55_CD137L_Antibody_012519
    Human T lymphoblastic leukemia cell line, Hut-78, was stained with CD137L (4-1BB Ligand)(clone 5F4) PerCP/Cyanine5.5 (filled histogram) or Mouse IgG1, ? PerCP/Cyanine5.5 isotype control (open histogram).
Compare all formats See PerCP/Cyanine5.5 spectral data
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311517 25 tests 132€
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311518 100 tests 306€
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Description

4-1BB ligand, also known as CD137L, is a 97 kD member of the TNF superfamily mainly expressed on APCs, activated B and T cells. It has been reported to be important in T cell proliferation and cytokine production through interaction with 4-1BB receptor. 4-1BB ligand appears to be able to act as a costimulatory molecule without the engagement of other costimulatory molecules such as CD28.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PerCP/Cyanine5.5 under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

* PerCP/Cyanine5.5 has a maximum absorption of 482 nm and a maximum emission of 690 nm.

Excitation Laser
Blue Laser (488 nm)
Application Notes

For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 311504) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated anti-mouse IgG second step (Cat. No. 405303), followed by SAv-PE (Cat. No. 405204)).

Additional Product Notes
BioLegend is in the process of converting the name PerCP/Cy5.5 to PerCP/Cyanine5.5. The dye molecule remains the same, so you should expect the same quality and performance from our PerCP/Cyanine5.5 products. Contact Technical Service if you have any questions.
Application References
  1. Gullo C, et al. 2010. PLoS One. 5:e10845. (FC) PubMed
RRID
AB_2749936 (BioLegend Cat. No. 311517)
AB_2749937 (BioLegend Cat. No. 311518)

Antigen Details

Structure
TNF superfamily, 97 kD
Distribution

Antigen presenting cells, activated B cells and T cells

Function
T cell proliferation, cytokine production
Ligand/Receptor
4-1BB (CDw137)
Cell Type
Antigen-presenting cells, B cells, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Akiba H, et al. 2000. J. Exp. Med. 191:375. 
2. Pollak KE, et al. 1995. Eur. J. Immunol. 25:488. 
3. DeBenedette MA, et al. 1997. J. Immunol. 158:551. 
4. Goodwin RG, et al. 1993. Eur. J. Immunol. 23:2631.

Gene ID
8744 View all products for this Gene ID
UniProt
View information about CD137L on UniProt.org

Related FAQs

How stable is PerCP/Cyanine5.5 tandem as compared to PerCP alone?

PerCP/Cyanine5.5 is quite photostable and also better than PerCP alone in withstanding fixation.

Go To Top Version: 2    Revision Date: 01-25-2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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  • PE/Dazzle™ 594 anti-human CD137L (4-1BB Ligand)

  • PerCP/Cyanine5.5 anti-human CD137L (4-1BB Ligand)

  • APC/Fire™ 750 anti-human CD137L (4-1BB Ligand)

  • PE/Cyanine7 anti-human CD137L (4-1BB Ligand)

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