PE/Cyanine7 anti-mouse IFN-γ Antibody

Pricing & Availability
Clone
XMG1.2 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF)
Isotype
Rat IgG1, κ
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Product Citations
publications
XMG1-2_PECyanine7_IFN-y_Antibody_1_041124
PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with B220 (RA3-6B2) APC and XMG1.2 PE/Cyanine7
  • XMG1-2_PECyanine7_IFN-y_Antibody_1_041124
    PMA/Ionomycin-stimulated (6hrs) C57BL/6 mouse splenocytes stained with B220 (RA3-6B2) APC and XMG1.2 PE/Cyanine7
Compare all formats See PE/Cyanine7 spectral data
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505825 25 µg 106€
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505826 100 µg 243€
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Description

IFN-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells.

Product Details
Technical Data Sheet (pdf)

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA1-4,11,14 or ELISPOT5 Detection: The biotinylated XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified R4-6A2 antibody (Cat. No. 505702/505706) as the capture antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard.
ELISA or ELISPOT Capture: The purified XMG1.2 antibody is useful as a capture antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with biotinylated R4-6A2 antibody (Cat. No. 505704) as the detection antibody and recombinant mouse IFN-γ (Cat. No. 575309) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture (Cat. No. 505812).
Flow Cytometry7,8,12,13,16: The fluorochrome-labeled XMG1.2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-γ-producing cells within mixed cell populations.
Neutralization1-3,9,10: The XMG1.2 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of mouse IFN-γ bioactivity in vivo and in vitro (Cat. No. 505812). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 505834) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
Additional reported applications (for the relevant formats) include: Western blotting, immunohistochemical staining of frozen tissue sections6,22,23, and immunocytochemistry.
Note: For testing mouse IFN-γ in serum, plasma or supernatant, BioLegend's ELISA Max™ Sets (Cat. No. 430801 to 430806) are specially developed and recommended.

Additional Product Notes

BioLegend is in the process of converting the name PE/Cy7 to PE/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our PE/Cyanine7 products. Please contact Technical Service if you have any questions.

Application References
  1. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA, Neut)
  2. Sander B, et al. 1993. J. Immunol. Meth. 166:201. (ELISA, Neut)
  3. Abrams J, et al. 1995. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.20. (ELISA, Neut)
  4. Yang X, et al. 1993. J. Immunoassay 14:129. (ELISA)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons, New York. Unit 6.19. (ELISPOT)
  6. Sander B, et al. 1991. Immunol. Rev. 119:65. (IHC)
  7. Ferrick D, et al. 1995. Nature 373:255. (FC)
  8. Ko SY, et al. 2005. J. Immunol. 175:3309. (FC) PubMed
  9. Peterson KE, et al. 2000. J. Virol. 74:5363. (Neut)
  10. DeKrey GK, et al. 1998. Infect. Immun. 66:827. (Neut)
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. (ELISA)
  12. Lawson BR, et al. 2007. J. Immunol. 178:5366. (FC)
  13. Lee JW, et al. 2006. Nature Immunol. 8:181. (FC) PubMed
  14. Xu G, et al. 2007. J. Immunol. 179:5358. (ELISA) PubMed
  15. Montfort M, et al.2004. J. Immunol. 173:4084. PubMed
  16. Haring JS, et al. 2008. J. Immunol. 180:2855. (FC) PubMed
  17. Jordan JM, et al. 2008. Infect Immun. 76:3717. PubMed
  18. Tonkin DR, et al. 2008. J. Immunol. 181:4516. PubMed
  19. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  20. Cui Y, et al. 2009. Invest. Ophth. Vis. Sci. 50:5811. (FC) PubMed
  21. Mykkanen OT, et al. 2014. PLoS One. 9:114790. PubMed
  22. Yokogawa M, et al. 2013. Mol. Carcinog. 52:760. (IHC)
  23. Mottram PL, et al. 1998. J Immunol. 161:602. (IHC)
Product Citations
  1. Laura C Burzynski et al. 2019. Immunity. 50(4):1033-1042 . PubMed
  2. Wang X, et al. 2019. Cell Res. 29:787. PubMed
  3. Marchelletta RR, et al. 2021. J Clin Invest. 131:. PubMed
  4. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  5. Harsha Krovi S, et al. 2020. Nat Commun. 4.790277778. PubMed
  6. Nakata J, et al. 2018. Oncotarget. 9:36029. PubMed
  7. He Y, et al. 2021. Infect Immun. 89:. PubMed
  8. Geng Z, et al. 2021. Nat Commun. 12:6584. PubMed
  9. Gern BH, et al. 2021. Cell Host Microbe. 29(4):594-606.e6. PubMed
  10. Lino AC et al. 2018. Immunity. 49(1):120-133 . PubMed
  11. Liu QZ, et al. 2018. Front Immunol. 1.131944444. PubMed
  12. Baldwin SL, et al. 2021. PLoS One. 16:e0247990. PubMed
  13. Mogilenko DA, et al. 2020. Immunity. 54(1):99-115.e12. PubMed
  14. He Y, et al. 2020. Sci Adv. 6:eaba7589. PubMed
  15. Wu J, et al. 2020. Cell Reports. 31(1):107484. PubMed
  16. Harel M, et al. 2020. Cell. 179(1):236-250.e18.. PubMed
  17. Lin H, et al. 2020. Front Microbiol. 1.330555556. PubMed
  18. Zhao B, et al. 2020. Nat Commun. 11:908. PubMed
  19. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  20. Álvaro de Mingo Pulido et al. 2018. Cancer cell. 33(1):60-74 . PubMed
  21. Saito S, et al. 2020. Nutrients. 12:. PubMed
  22. Nagai Y, et al. 2019. Front Immunol. 10:174. PubMed
  23. Kosack L, et al. 2017. Sci Rep. 7:11289. PubMed
  24. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  25. Christopher S Garris et al. 2018. Immunity. 49(6):1148-1161 . PubMed
  26. Wu J, et al. 2020. Sci Adv. 6:eaba3458. PubMed
  27. Linnerbauer M, et al. 2022. Front Immunol. 12:800128. PubMed
  28. Zhou HF, et al. 2022. Front Pharmacol. 13:778755. PubMed
  29. Yang W, et al. 2020. Mucosal Immunol. 13:788. PubMed
  30. Zhang H, et al. 2020. Cancer Cell. 37(1):37-54.e9.. PubMed
  31. Lercher A, et al. 2019. Immunity. 51:1074. PubMed
  32. Britton GJ et al. 2019. Immunity. 50(1):212-224 . PubMed
  33. Nagarajan D, et al. 2021. Immunol Cell Biol. 99:972. PubMed
  34. Zhan Y, et al. 2021. JCI Insight. 6:. PubMed
  35. Cousin N, et al. 2021. Cancer Res. 81:4133. PubMed
  36. Yu AI, et al. 2020. Cell Rep. 107471:31. PubMed
  37. Marangoni F, et al. 2021. Cell. . PubMed
  38. Voisinne G, et al. 2022. Nat Immunol. 23:1355. PubMed
  39. Glennie N, et al. 2015. J Exp Med. 212: 1405-1414. PubMed
  40. Dumas AA, et al. 2020. EMBO J. 39:e103790. PubMed
  41. Devos M, et al. 2020. J Exp Med. 217:00:00. PubMed
  42. Maluski M, et al. 2019. J Clin Invest. 129:5108. PubMed
  43. Deng Z, et al. 2017. Oncogene. 36:639. PubMed
  44. Colliou N, et al. 2018. Gut Microbes. 9:279. PubMed
  45. Yang L, et al. 2021. Cell Death Differ. 28:2616. PubMed
  46. Tuganbaev T, et al. 2020. Cell. 182(6):1441-1459.e21. PubMed
  47. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  48. Sawant D, et al. 2014. J Immunol. 192:2904. PubMed
  49. Ma W, et al. 2017. Sci Rep. . 10.1038/s41598-017-15661-6. PubMed
  50. Overacre-Delgoffe AE, et al. 2017. Cell. 169:1130. PubMed
  51. Schadt L, et al. 2020. Cell Reports. 29(5):1236-1248.e7.. PubMed
  52. Cho S, et al. 2017. Sci Rep. 7:35. PubMed
  53. Bogie JF, et al. 2020. Ther Adv Chronic Dis. 11:2040622320947378. PubMed
  54. Tello-Lafoz M, et al. 2021. Immunity. 54(5):1037-1054.e7. PubMed
  55. Jing Y, et al. 2020. Sci Adv. 6:eaax9455. PubMed
  56. Habib S, et al. 2018. Infect Immun. 86:e00019. PubMed
  57. Zhao X, et al. 2022. Nat Protoc. 17:2240. PubMed
  58. Silver J, et al. 2020. Methods Mol Biol. 2121:115. PubMed
  59. Stotesbury C, et al. 2020. J Immunol. 204:1582. PubMed
  60. Xu S, et al. 2021. Immunity. . PubMed
  61. Volberding PJ, et al. 2021. Cell Reports. 35(8):109160. PubMed
  62. Harb H, et al. 2021. Immunity. 54(6):1186-1199.e7. PubMed
  63. Jiang J, et al. 2017. Arthritis Res Ther. 10.1186/s13075-017-1261-9. PubMed
  64. Meadows D, et al. 2015. PLoS One. 10: 0143738. PubMed
  65. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  66. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
  67. Wu B, et al. 2020. Int J Biol Sci. 16:1526. PubMed
  68. Wang Y, et al. 2020. Cell Death Dis. 1.169444444. PubMed
  69. Frost JN, et al. 2021. Med (N Y). 2:164. PubMed
  70. Saigusa R, et al. 2018. Arthritis Res Ther. 20:23. PubMed
  71. Xi‐Zhi J Guo et al. 2018. Immunity. 49(3):531-544 . PubMed
  72. Fu S, et al. 2020. Nat Commun. 0.7625. PubMed
  73. Zhang W, et al. 2020. Nat Commun. 11:1187. PubMed
  74. Abhyankar MM, et al. 2018. NPJ Vaccines. 3:22. PubMed
  75. Zhou J, et al. 2022. Int J Oncol. 61: . PubMed
  76. Swanson P, et al. 2016. PLoS Pathog. 12:e1006022. PubMed
  77. Holm J, et al. 2015. PLoS One. 10:125495. PubMed
  78. Li C, et al. 2018. Cell. 174:285. PubMed
  79. Resende M, et al. 2019. J Immunol. 203:2451. PubMed
  80. Oliveira AC et al. 2017. eLife. 6 pii: e30883. PubMed
  81. Minns D, et al. 2021. Nat Commun. 12:1285. PubMed
  82. Dhar P, et al. 2021. Commun Biol. 4:905. PubMed
  83. Roy S, et al. 2021. Nat Commun. 12:3182. PubMed
  84. Sun X, et al. 2021. Nature. 599:673. PubMed
  85. Zhao X, et al. 2022. iScience. 25:104690. PubMed
  86. Friedman R, et al. 2014. Proc Natl Acad Sci U S A. 111:9223. PubMed
  87. Huang H, et al. 2021. Cell. 184(5):1245-1261.e21. PubMed
  88. Sun X, et al. 2017. Microbes Infect. 10.1016/j.micinf.2017.08.007. PubMed
  89. Chen Y, et al. 2021. Nat Immunol. 22:996. PubMed
  90. Leary N, et al. 2022. J Extracell Vesicles. 11:e12197. PubMed
  91. Lee J, et al. 2007. Nat Immunol. 8:181. PubMed
  92. Yu H, et al. 2021. Cell Death Dis. 13:1. PubMed
  93. Smith KJ, et al. 2022. PLoS Biol. 20:e3001554. PubMed
  94. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
  95. Imbratta C, et al. 2019. Sci Rep. 9:6135. PubMed
  96. Stotesbury C, et al. 2020. Aging Cell. 19:e13170. PubMed
  97. Malenica I, et al. 2021. Nat Commun. 12:5209. PubMed
  98. Yue T, et al. 2022. Cell Death Differ. 29:218. PubMed
  99. Siolas D, et al. 2021. Cell Reports. 36:109578. PubMed
  100. Srivastava S, et al. 2020. Cancer Cell. 39(2):193-208.e10. PubMed
  101. Wen J, et al. 2015. J Pharmacol Sci. 128: 116-124. PubMed
  102. Guo Y, et al. 2021. Nat Immunol. 22:746. PubMed
  103. Gardner A, et al. 2022. J Immunother Cancer. 10:. PubMed
  104. Zhang M, et al. 2022. iScience. 25:104490. PubMed
  105. Jaeger N, et al. 2020. Cell Rep. 33:108331. PubMed
  106. Zander R, et al. 2020. Immunity. 51(6):1028-1042.e4.. PubMed
  107. Wolf Y, et al. 2019. Cell. 179:219. PubMed
  108. Omenetti S, et al. 2019. Immunity. 51:77. PubMed
  109. Wang Y, et al. 2022. Nat Cell Biol. 24:230. PubMed
  110. Kalim KW, et al. 2021. Front Immunol. 12:726393. PubMed
  111. Du Z, et al. 2020. J Allergy Clin Immunol. . PubMed
  112. Jackson C, et al. 2016. Clin Cancer Res. 22: 1161 - 1172. PubMed
  113. Olguín J, et al. 2015. Microbes Infect. 17: 586-595. PubMed
RRID
AB_1595591 (BioLegend Cat. No. 505825)
AB_2295770 (BioLegend Cat. No. 505826)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APCs
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu. Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, FGF-basic, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
15978 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

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Go To Top Version: 1    Revision Date: 11-30-2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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